Abstract
Transducing bacteriophages CP-51 and CP-53 were compared. Unlike CP-51, CP-53 appeared to be a lysogenizing phage. CP-51 gave greater frequencies of co-transduction for linked markers than did CP-53. CP-51 was found to be a larger phage which carried more deoxyribonucleic acid (DNA) than CP-53. CP-51 DNA contained about 43% guanine plus cytosine and in addition contained 5-hydroxymethyluracil in place of thymine. CP-53 DNA contained no unusual bases; its guanine plus cytosine content was 37%.
Full text
PDFImages in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ALTENBERN R. A., STULL H. B. EDEMA FACTOR AND PHOSPHOLIPASE RELEASE BY A STRAIN OF BACILLUS CEREUS. Can J Microbiol. 1964 Oct;10:717–725. doi: 10.1139/m64-091. [DOI] [PubMed] [Google Scholar]
- BOTT K., STRAUSS B. THE CARRIER STATE OF BACILLUS SUBTILIS INFECTED WITH THE TRANSDUCING BACTERIOPHAGE SP10. Virology. 1965 Feb;25:212–225. doi: 10.1016/0042-6822(65)90200-x. [DOI] [PubMed] [Google Scholar]
- BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Barksdale L. I. : Lysogenic Conversions in Bacteria. Bacteriol Rev. 1959 Dec;23(4):202–212. doi: 10.1128/br.23.4.202-212.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
- De Ley J. Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. J Bacteriol. 1970 Mar;101(3):738–754. doi: 10.1128/jb.101.3.738-754.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Espejo R. T., Canelo E. S., Sinsheimer R. L. DNA of bacteriophage PM2: a closed circular double-stranded molecule. Proc Natl Acad Sci U S A. 1969 Aug;63(4):1164–1168. doi: 10.1073/pnas.63.4.1164. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Freifelder D. Molecular weights of coliphages and coliphage DNA. IV. Molecular weights of DNA from bacteriophages T4, T5 and T7 and the general problem of determination of M. J Mol Biol. 1970 Dec 28;54(3):567–577. doi: 10.1016/0022-2836(70)90127-0. [DOI] [PubMed] [Google Scholar]
- GOLDBERG I. D., KENG J. G., THORNE C. B. ISOLATION OF AUXOTROPHS OF BACILLUS CEREUS. J Bacteriol. 1965 May;89:1441–1441. doi: 10.1128/jb.89.5.1441-1441.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hershey A. D., Burgi E., Ingraham L. COHESION OF DNA MOLECULES ISOLATED FROM PHAGE LAMBDA. Proc Natl Acad Sci U S A. 1963 May;49(5):748–755. doi: 10.1073/pnas.49.5.748. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jonasson J., Rutberg L., Young F. E. Lysogenic Conversion in Bacillus amyloliquefaciens H Affecting Viral Adsorption. J Virol. 1969 Sep;4(3):309–310. doi: 10.1128/jvi.4.3.309-310.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Lang D., Mitani M. Simplified quantitative electron microscopy of biopolymers. Biopolymers. 1970;9(3):373–379. doi: 10.1002/bip.1970.360090310. [DOI] [PubMed] [Google Scholar]
- Lang D. Molecular weights of coliphages and coliphage DNA. 3. Contour length and molecular weight of DNA from bacteriophages T4, T5 and T7, and from bovine papilloma virus. J Mol Biol. 1970 Dec 28;54(3):557–565. doi: 10.1016/0022-2836(70)90126-9. [DOI] [PubMed] [Google Scholar]
- MANDELL J. D., HERSHEY A. D. A fractionating column for analysis of nucleic acids. Anal Biochem. 1960 Jun;1:66–77. doi: 10.1016/0003-2697(60)90020-8. [DOI] [PubMed] [Google Scholar]
- Pitout M. J., Conradie J. D., Van Rensburg A. J. Relationship between the sedimentation coefficient and molecular weight of bacteriophages. J Gen Virol. 1969 Jun;4(4):577–583. doi: 10.1099/0022-1317-4-4-577. [DOI] [PubMed] [Google Scholar]
- Rao R. N. Bacteriophage P22 controlled exclusion in Salmonella typhimurium. J Mol Biol. 1968 Aug 14;35(3):607–622. doi: 10.1016/s0022-2836(68)80017-8. [DOI] [PubMed] [Google Scholar]
- TAYLOR M. J., THORNE C. B. TRANSDUCTION OF BACILLUS LICHENIFORMIS AND BACILLUS SUBTILIS BY EACH OF TWO PHAGES. J Bacteriol. 1963 Sep;86:452–461. doi: 10.1128/jb.86.3.452-461.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- THORNE C. B. Transduction in Bacillus subtilis. J Bacteriol. 1962 Jan;83:106–111. doi: 10.1128/jb.83.1.106-111.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thorne C. B., Stull H. B. Factors affecting transformation of Bacillus licheniformis. J Bacteriol. 1966 Mar;91(3):1012–1020. doi: 10.1128/jb.91.3.1012-1020.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thorne C. B. Transducing bacteriophage for Bacillus cereus. J Virol. 1968 Jul;2(7):657–662. doi: 10.1128/jvi.2.7.657-662.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thorne C. B. Transduction in Bacillus cereus and Bacillus anthracis. Bacteriol Rev. 1968 Dec;32(4 Pt 1):358–361. [PMC free article] [PubMed] [Google Scholar]
- Tyeryar F. J., Jr, Taylor M. J., Lawton W. D., Goldberg I. D. Cotransduction and cotransformation of genetic markers in Bacillus subtilis and Bacillus licheniformis. J Bacteriol. 1969 Nov;100(2):1027–1036. doi: 10.1128/jb.100.2.1027-1036.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WYATT G. R., COHEN S. S. A new pyrimidine base from bacteriophage nucleic acids. Nature. 1952 Dec 20;170(4338):1072–1073. doi: 10.1038/1701072a0. [DOI] [PubMed] [Google Scholar]
- WYATT G. R. The purine and pyrimidine composition of deoxypentose nucleic acids. Biochem J. 1951 May;48(5):584–590. doi: 10.1042/bj0480584. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yamagishi H. Single strand interruptions in PBS 1 bacteriophage DNA molecule. J Mol Biol. 1968 Aug 14;35(3):623–633. doi: 10.1016/s0022-2836(68)80018-x. [DOI] [PubMed] [Google Scholar]
- Yelton D. B., Thorne C. B. Transduction in Bacillus cereus by each of two bacteriophages. J Bacteriol. 1970 May;102(2):573–579. doi: 10.1128/jb.102.2.573-579.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ZINDER N. D. Bacterial transduction. J Cell Physiol Suppl. 1955 May;45(Suppl 2):23–49. doi: 10.1002/jcp.1030450504. [DOI] [PubMed] [Google Scholar]