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. 1972 Jan;9(1):22–28. doi: 10.1128/jvi.9.1.22-28.1972

Virus-Induced Interference in Heterologously Infected HeLa Cells

M Doyle 1, J J Holland 1
PMCID: PMC356258  PMID: 4333542

Abstract

In doubly infected HeLa cells, poliovirus type 1 rapidly and completely dominates vesicular stomatitis virus (VSV) plaque-forming unit production. Poliovirus type 1 shuts off incorporation of amino acids into VSV-specific proteins within 2 hr after superinfection of cells already committed to massive synthesis of VSV proteins. However, poliovirus type 1 appears to have little, if any, direct effect upon incorporation of uridine into VSV-directed ribonucleic acid (RNA) synthesis. Poliovirus apparently interferes with VSV virion production only at the level of translation of viral messenger RNA, although it interferes with host cell macromolecular syntheses at the levels of translation and transcription.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bishop D. H., Claybrook J. R., Spiegelman S. Electrophoretic separation of viral nucleic acids on polyacrylamide gels. J Mol Biol. 1967 Jun 28;26(3):373–387. doi: 10.1016/0022-2836(67)90310-5. [DOI] [PubMed] [Google Scholar]
  2. CORDS C. E., HOLLAND J. J. INTERFERENCE BETWEEN ENTEROVIRUSES AND CONDITIONS EFFECTING ITS REVERSAL. Virology. 1964 Feb;22:226–234. doi: 10.1016/0042-6822(64)90007-8. [DOI] [PubMed] [Google Scholar]
  3. Choppin P. W., Holmes K. V. Replication of SV5 RNA and the effects of superinfection with poliovirus. Virology. 1967 Nov;33(3):442–451. doi: 10.1016/0042-6822(67)90119-5. [DOI] [PubMed] [Google Scholar]
  4. Cordell-Stewart B., Taylor M. W. Effect of double-stranded viral RNA on mammalian cells in culture. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1326–1330. doi: 10.1073/pnas.68.6.1326. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HOLLAND J. J., McLAREN L. C. Improved method for staining cell monolayers for virus plaque counts. J Bacteriol. 1959 Oct;78:596–597. doi: 10.1128/jb.78.4.596-597.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hunt T., Ehrenfeld E. Cytoplasm from poliovirus-infected HeLa cells inhibits cell-free haemoglobin synthesis. Nat New Biol. 1971 Mar 17;230(11):91–94. doi: 10.1038/newbio230091a0. [DOI] [PubMed] [Google Scholar]
  7. Kiehn E. D., Holland J. J. Membrane and nonmembrane proteins of mammalian cells. Synthesis, turnover, and size distribution. Biochemistry. 1970 Apr 14;9(8):1716–1728. doi: 10.1021/bi00810a010. [DOI] [PubMed] [Google Scholar]
  8. LEDINKO N. An analysis of interference between active polioviruses types 1 and 2 in HeLa cells. Virology. 1963 May;20:29–44. doi: 10.1016/0042-6822(63)90138-7. [DOI] [PubMed] [Google Scholar]
  9. LEDINKO N., MELNICK J. L. Interference between poliomyelitis viruses in tissue culture. J Exp Med. 1954 Sep 1;100(3):247–267. doi: 10.1084/jem.100.3.247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McCormick W., Penman S. Replication of mengovirus in HeLa cells preinfected with nonreplicating poliovirus. J Virol. 1968 Sep;2(9):859–864. doi: 10.1128/jvi.2.9.859-864.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mudd J. A., Summers D. F. Protein synthesis in vesicular stomatitis virus-infected HeLa cells. Virology. 1970 Oct;42(2):328–340. doi: 10.1016/0042-6822(70)90277-1. [DOI] [PubMed] [Google Scholar]
  12. Petric M., Prevec L. Vesicular stomatitis virus--a new interfering particle, intracellular structures, and virus-specific RNA. Virology. 1970 Aug;41(4):615–630. doi: 10.1016/0042-6822(70)90427-7. [DOI] [PubMed] [Google Scholar]
  13. Stampfer M., Baltimore D., Huang A. S. Ribonucleic acid synthesis of vesicular stomatitis virus. I. Species of ribonucleic acid found in Chinese hamster ovary cells infected with plaque-forming and defective particles. J Virol. 1969 Aug;4(2):154–161. doi: 10.1128/jvi.4.2.154-161.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wagner R. R., Snyder R. M., Yamazaki S. Proteins of vesicular stomatitis virus: kinetics and cellular sites of synthesis. J Virol. 1970 May;5(5):548–558. doi: 10.1128/jvi.5.5.548-558.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Willems M., Penman S. The mechanism of host cell protein synthesis inhibition by poliovirus. Virology. 1966 Nov;30(3):355–367. doi: 10.1016/0042-6822(66)90114-0. [DOI] [PubMed] [Google Scholar]
  16. ZIMMERMAN E. F., HEETER M., DARNELL J. E. RNA synthesis in poliovirus-infected cells. Virology. 1963 Mar;19:400–408. doi: 10.1016/0042-6822(63)90080-1. [DOI] [PubMed] [Google Scholar]

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