Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1972 Jan;9(1):41–51. doi: 10.1128/jvi.9.1.41-51.1972

Synthesis and Assembly of Simian Virus 40 I. Differential Synthesis of Intact Virions and Empty Shells

Harvey L Ozer 1
PMCID: PMC356260  PMID: 4333544

Abstract

Intact virions and empty shells of simian virus 40 may be rapidly separated from each other and from cell contaminants by a procedure employing a CsCl cushion. This approach permits quantitation of their respective syntheses in infected cells labeled with radioactive amino acids. As much as 5 to 10% of the total acid-precipitable radioactive lysine in infected cell extracts was incorporated into viral particles in a two-hour pulse late in infection. Evidence for multiple origins of empty shells is presented. Some of the empty shells result from breakdown of intact virions. However, empty shells can also form independently of intact virions. First, labeling for periods of 15 min to 2 hr late in the course of infection results in preferential incorporation of 3H-lysine into empty shells. Secondly, treatment with the deoxyribonucleic acid inhibitor cytosine-β-d-arabinofuranoside late in infection results in a 50% inhibition in the rate of formation of intact virions with minimal reduction in the rate of appearance of empty shells.

Full text

PDF
46

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderer F. A., Koch M. A., Schlumberger H. D. Structure of simian virus 40. 3. Alkaline degradation of the virus particle. Virology. 1968 Mar;34(3):452–458. doi: 10.1016/0042-6822(68)90065-2. [DOI] [PubMed] [Google Scholar]
  2. Anderer F. A., Schlumberger H. D., Koch M. A., Frank H., Eggers H. J. Structure of simian virus 40. II. Symmetry and components of the virus particle. Virology. 1967 Jul;32(3):511–523. doi: 10.1016/0042-6822(67)90303-0. [DOI] [PubMed] [Google Scholar]
  3. BLACK P. H., CRAWFORD E. M., CRAWFORD L. V. THE PURIFICATION OF SIMIAN VIRUS 40. Virology. 1964 Nov;24:381–387. doi: 10.1016/0042-6822(64)90175-8. [DOI] [PubMed] [Google Scholar]
  4. Barban S., Goor R. S. Structural proteins of simian virus 40. J Virol. 1971 Feb;7(2):198–203. doi: 10.1128/jvi.7.2.198-203.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Estes M. K., Huang E. S., Pagano J. S. Structural polypeptides of simian virus 40. J Virol. 1971 May;7(5):635–641. doi: 10.1128/jvi.7.5.635-641.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Friedmann T., Haas M. Rapid concentration and purification of polyoma virus and SV40 with polyethylene glycol. Virology. 1970 Sep;42(1):248–250. doi: 10.1016/0042-6822(70)90263-1. [DOI] [PubMed] [Google Scholar]
  7. GRANBOULAN N., TOURNIER P., WICKER R., BERNHARD W. An electron microscope study of the development of SV40 virus. J Cell Biol. 1963 May;17:423–441. doi: 10.1083/jcb.17.2.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Girard M., Marty L., Suarez F. Capsid proteins of Simian virus 40. Biochem Biophys Res Commun. 1970 Jul 13;40(1):97–102. doi: 10.1016/0006-291x(70)91051-x. [DOI] [PubMed] [Google Scholar]
  9. Jacobson M. F., Baltimore D. Morphogenesis of poliovirus. I. Association of the viral RNA with coat protein. J Mol Biol. 1968 Apr 28;33(2):369–378. doi: 10.1016/0022-2836(68)90195-2. [DOI] [PubMed] [Google Scholar]
  10. Koch M. A., Becht H., Anderer F. A. Structure of simian virus 40. V. Localization of the C-type polypeptide chains. Virology. 1971 Jan;43(1):235–242. doi: 10.1016/0042-6822(71)90241-8. [DOI] [PubMed] [Google Scholar]
  11. Koch M. A., Eggers H. J., Anderer F. A., Schlumberger H. D., Frank H. Structure of simian virus 40. I. Purification and physical characterization of the virus particle. Virology. 1967 Jul;32(3):503–510. doi: 10.1016/0042-6822(67)90302-9. [DOI] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Moss B., Salzman N. P. Sequential protein synthesis following vaccinia virus infection. J Virol. 1968 Oct;2(10):1016–1027. doi: 10.1128/jvi.2.10.1016-1027.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ozer H. L., Takemoto K. K., Kirschstein R. L., Axelrod D. Immunochemical characterization of plaque mutants of simian virus 40. J Virol. 1969 Jan;3(1):17–24. doi: 10.1128/jvi.3.1.17-24.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ozer H. L., Tegtmeyer P. Synthesis and assembly of simian virus 40. II. Synthesis of the major capsid protein and its incorporation into viral particles. J Virol. 1972 Jan;9(1):52–60. doi: 10.1128/jvi.9.1.52-60.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. PATTERSON M. S., GREENE R. C. MEASUREMENT OF LOW ENERGY BETA-EMITTERS IN AQUEOUS SOLUTION BY LIQUID SCINTILLATION COUNTING OF EMULSIONS. Anal Chem. 1965 Jun;37:854–857. doi: 10.1021/ac60226a017. [DOI] [PubMed] [Google Scholar]
  17. RAPP F., BUTEL J. S., FELDMAN L. A., KITAHARA T., MELNICK J. L. DIFFERENTIAL EFFECTS OF INHIBITORS ON THE STEPS LEADING TO THE FORMATION OF SV40 TUMOR AND VIRUS ANTIGENS. J Exp Med. 1965 Jun 1;121:935–944. doi: 10.1084/jem.121.6.935. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Robb J. A., Martin R. G. Genetic analysis of simian virus 40. I. Description of microtitration and replica-plating techniques for virus. Virology. 1970 Aug;41(4):751–760. doi: 10.1016/0042-6822(70)90439-3. [DOI] [PubMed] [Google Scholar]
  19. Shapiro A. L., Viñuela E., Maizel J. V., Jr Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochem Biophys Res Commun. 1967 Sep 7;28(5):815–820. doi: 10.1016/0006-291x(67)90391-9. [DOI] [PubMed] [Google Scholar]
  20. Summers D. F., Maizel J. V., Jr, Darnell J. E., Jr Evidence for virus-specific noncapsid proteins in poliovirus-infected HeLa cells. Proc Natl Acad Sci U S A. 1965 Aug;54(2):505–513. doi: 10.1073/pnas.54.2.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Takemoto K. K., Kirschstein R. L., Habel K. Mutants of simian virus 40 differing in plaque size, oncogenicity, and heat sensitivity. J Bacteriol. 1966 Oct;92(4):990–994. doi: 10.1128/jb.92.4.990-994.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Tegtmeyer P., Dohan C., Jr, Reznikoff C. Inactivating and mutagenic effects of nitrosoguanidine on simian virus 40. Proc Natl Acad Sci U S A. 1970 Jul;66(3):745–752. doi: 10.1073/pnas.66.3.745. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Tegtmeyer P., Ozer H. L. Temperature-sensitive mutants of simian virus 40: infection of permissive cells. J Virol. 1971 Oct;8(4):516–524. doi: 10.1128/jvi.8.4.516-524.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES