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. 1972 May;9(5):738–745. doi: 10.1128/jvi.9.5.738-745.1972

Genetic Relatedness of Type 1 and Type 2 Herpes Simplex Viruses

Elliott Kieff 1,2, Bill Hoyer 1,2, Steven Bachenheimer 1,2, Bernard Roizman 1,2
PMCID: PMC356368  PMID: 4337161

Abstract

The extent of homology between herpes simplex virus1 and2 (HSV-1 and HSV-2) deoxyribonucleic acid (DNA) was measured in two ways: (i) by determination of the relative rate of hybridization of labeled HSV-1 and HSV-2 DNA to excess unlabeled HSV-1 or HSV-2 DNA immobilized on filters and (ii) by determination of the rate of hybridization of labeled HSV-1 and HSV-2 DNA to excess unlabeled HSV-1 or HSV-2 DNA in solution. Approximately 40% of HSV-1 and HSV-2 DNA is homologous at hybridization temperatures 25 C below the melting temperature (Tm) of HSV DNA (liquid-filter annealing). Lowering the temperature to 34 C below the Tm increased the extent of homology to 46% (liquid annealing). The extent of base-pairing in HSV-1-HSV-2 heteroduplex DNA was determined by thermal chromatography on hydroxyapatite. Heteroduplexes of HSV-1 and HSV-2 DNA eluted in a single peak whose midpoint (Te50) was 10 C below that of the homoduplex. Conspicuously absent were heteroduplexes that eluted at more than 15 C below the Te50 of the homoduplex. The data indicate the existence of a variable region of DNA (54%) with very little, if any, homology and an invariable region (46%) with relatively good (85%) matching of base pairs.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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