Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1972 Jun;9(6):973–980. doi: 10.1128/jvi.9.6.973-980.1972

Synthesis In Vitro of Type 5 Adenovirus Capsid Proteins 1

James M Wilhelm a, Harold S Ginsberg a
PMCID: PMC356403  PMID: 5064439

Abstract

Reaction mixtures containing cytoplasmic extracts and ribosomal fractions prepared from KB cells infected with type 5 adenovirus were able to carry out incorporation of amino acids into protein. The in vitro product included proteins which reacted specifically with antisera to adenovirus capsid proteins; in control experiments with extracts from uninfected cells, no reactions with the antisera were found. The viral proteins were synthesized in vitro on small polyribosomes, were released from them, and significant numbers of the free polypeptides were assembled in vitro into multimeric adenovirus capsid structures.

Full text

PDF
976

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOYER G. S., DENNY F. W., Jr, GINSBERG H. S. Sequential cellular changes produced by types 5 and 7 adenoviruses in HeLa cells and in human amniotic cells; cytological studies aided by fluorescein-labelled antibody. J Exp Med. 1959 Nov 1;110:827–844. doi: 10.1084/jem.110.5.827. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BOYER G. S., LEUCHTENBERGER C., GINSBERG H. S. Cytological and cytochemical studies of HeLa cells infected with adeno-viruses. J Exp Med. 1957 Mar 1;105(3):195–216. doi: 10.1084/jem.105.3.195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bello L. J., Ginsberg H. S. Relationship between deoxyribonucleic acid-like ribonucleic acid synthesis and inhibition of host protein synthesis in type 5 adenovirus-infected KB cells. J Virol. 1969 Feb;3(2):106–113. doi: 10.1128/jvi.3.2.106-113.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Caffier H., Raskas H. J., Parsons T. J., Green M. Initiation of mammalian viral protein synthesis. Nat New Biol. 1971 Feb 24;229(8):239–241. doi: 10.1038/newbio229239a0. [DOI] [PubMed] [Google Scholar]
  5. Colombo B., Felicetti L., Baglioni C. Inhibition of protein synthesis in reticulocytes by antibiotics. I. Effects on polysomes. Biochim Biophys Acta. 1966 Apr 18;119(1):109–119. doi: 10.1016/0005-2787(66)90043-8. [DOI] [PubMed] [Google Scholar]
  6. Ginsberg H. S., Pereira H. G., Valentine R. C., Wilcox W. C. A proposed terminology for the adenovirus antigens and virion morphological subunits. Virology. 1966 Apr;28(4):782–783. doi: 10.1016/0042-6822(66)90271-6. [DOI] [PubMed] [Google Scholar]
  7. Lawrence W. C., Ginsberg H. S. Intracellular uncoating of type 5 adenovirus deoxyribonucleic acid. J Virol. 1967 Oct;1(5):851–867. doi: 10.1128/jvi.1.5.851-867.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lazarides E., Lukens L. N. Collagen synthesis on polysomes in vivo and in vitro. Nat New Biol. 1971 Jul 14;232(28):37–40. doi: 10.1038/newbio232037a0. [DOI] [PubMed] [Google Scholar]
  9. Levine A. J., Ginsberg H. S. Mechanism by which fiber antigen inhibits multiplication of type 5 adenovirus. J Virol. 1967 Aug;1(4):747–757. doi: 10.1128/jvi.1.4.747-757.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lin S. Y., Mosteller R. D., Hardesty B. The mechanism of sodium fluoride and cycloheximide inhibition of hemoglobin biosynthesis in the cell-free reticulocyte system. J Mol Biol. 1966 Oct 28;21(1):51–69. doi: 10.1016/0022-2836(66)90079-9. [DOI] [PubMed] [Google Scholar]
  11. Lonberg-Holm K., Philipson L. Early events of virus-cell interaction in an adenovirus system. J Virol. 1969 Oct;4(4):323–338. doi: 10.1128/jvi.4.4.323-338.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Maizel J. V., Jr, White D. O., Scharff M. D. The polypeptides of adenovirus. II. Soluble proteins, cores, top components and the structure of the virion. Virology. 1968 Sep;36(1):126–136. doi: 10.1016/0042-6822(68)90122-0. [DOI] [PubMed] [Google Scholar]
  13. ORNSTEIN L. Mitochondrial and nuclear interaction. J Biophys Biochem Cytol. 1956 Jul 25;2(4 Suppl):351–352. doi: 10.1083/jcb.2.4.351. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. PEREIRA H. G., ALLISON A. C., BALFOUR B. Multiplication of adenovirus type 5 studied by infectivity titrations and by the fluorescent antibody technique. Virology. 1959 Mar;7(3):300–314. doi: 10.1016/0042-6822(59)90200-4. [DOI] [PubMed] [Google Scholar]
  15. Pettersson U., Philipson L., Höglund S. Structural proteins of adenoviruses. I. Purification and characterization of the adenovirus type 2 hexon antigen. Virology. 1967 Dec;33(4):575–590. doi: 10.1016/0042-6822(67)90057-8. [DOI] [PubMed] [Google Scholar]
  16. Pettersson U., Philipson L., Höglund S. Structural proteins of adenoviruses. II. Purification and characterization of the adenovirus type 2 fiber antigen. Virology. 1968 Jun;35(2):204–215. doi: 10.1016/0042-6822(68)90261-4. [DOI] [PubMed] [Google Scholar]
  17. Roumiantzeff M., Maizel J. V., Jr, Summers D. F. Comparison of polysomal structures of uninfected and poliovirus infected HeLa cells. Virology. 1971 May;44(2):239–248. doi: 10.1016/0042-6822(71)90256-x. [DOI] [PubMed] [Google Scholar]
  18. Roumiantzeff M., Summers D. F., Maizel J. V., Jr In vitro protein synthetic activity of membrane-bound poliovirus polyribosomes. Virology. 1971 May;44(2):249–258. doi: 10.1016/0042-6822(71)90257-1. [DOI] [PubMed] [Google Scholar]
  19. Velicer L. F., Ginsberg H. S. Cytoplasmic synthesis of type 5 adenovirus capsid proteins. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1264–1271. doi: 10.1073/pnas.61.4.1264. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Velicer L. F., Ginsberg H. S. Synthesis, transport, and morphogenesis of type adenovirus capsid proteins. J Virol. 1970 Mar;5(3):338–352. doi: 10.1128/jvi.5.3.338-352.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. WARNER J. R., KNOPF P. M., RICH A. A multiple ribosomal structure in protein synthesis. Proc Natl Acad Sci U S A. 1963 Jan 15;49:122–129. doi: 10.1073/pnas.49.1.122. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES