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. 1972 Aug;10(2):234–243. doi: 10.1128/jvi.10.2.234-243.1972

Dissociation of Vesicular Stomatitis Virus and Relation of the Virion Proteins to the Viral Transcriptase

D H L Bishop 1, Polly Roy 1
PMCID: PMC356455  PMID: 4342241

Abstract

A procedure has been developed for the sequential removal and purification of the glycoprotein and membrane protein of vesicular stomatitis virus (VSV). Neither of these proteins exhibited transcriptase activity. All of the activity was recovered in the ribonucleic acid (RNA)-ribonucleoprotein complex of VSV, which also has four other minor proteins associated with it. During transcription of 41% of the RNA of a virus preparation, no dissociation of the ribonucleoprotein from the viral RNA was observed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aaslestad H. G., Clark H. F., Bishop D. H., Koprowski H. Comparison of the ribonucleic acid polymerases of two rhabdoviruses, Kern Canyon virus and vesicular stomatitis virus. J Virol. 1971 Jun;7(6):726–735. doi: 10.1128/jvi.7.6.726-735.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Albertsson P. A. Partition of cell particles and macromolecules in polymer two-phase systems. Adv Protein Chem. 1970;24:309–341. doi: 10.1016/s0065-3233(08)60244-2. [DOI] [PubMed] [Google Scholar]
  3. Baltimore D., Huang A. S., Stampfer M. Ribonucleic acid synthesis of vesicular stomatitis virus, II. An RNA polymerase in the virion. Proc Natl Acad Sci U S A. 1970 Jun;66(2):572–576. doi: 10.1073/pnas.66.2.572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bishop D. H. Complete transcription by the transcriptase of vesicular stomatitis virus. J Virol. 1971 Apr;7(4):486–490. doi: 10.1128/jvi.7.4.486-490.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bishop D. H., Roy P. Kinetics of RNA synthesis by vesicular stomatitis virus particles. J Mol Biol. 1971 May 14;57(3):513–527. doi: 10.1016/0022-2836(71)90106-9. [DOI] [PubMed] [Google Scholar]
  6. Bishop D. H., Roy P. Properties of the product synthesized by vesicular stomatitis virus particles. J Mol Biol. 1971 Jun 28;58(3):799–814. doi: 10.1016/0022-2836(71)90041-6. [DOI] [PubMed] [Google Scholar]
  7. Brown F., Cartwright B., Almeida J. D. The antigens of vesicular stomatitis virus. I. Separation and immunogenicity of three complement-fixing components. J Immunol. 1966 Mar;96(3):537–545. [PubMed] [Google Scholar]
  8. Brown F., Cartwright B., Crick J., Smale C. J. Infective virus substructure from vesicular stomatitis virus. J Virol. 1967 Apr;1(2):368–373. doi: 10.1128/jvi.1.2.368-373.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Brown F., Cartwright B., Smale C. J. The antigens of vesicular stomatitis virus. 3. Structure and immunogenicity of antigens derived from the virion by treatment with Tween and ether. J Immunol. 1967 Jul;99(1):171–177. [PubMed] [Google Scholar]
  10. Cartwright B., Smale C. J., Brown F. Dissection of vesicular stomatitis virus into the infective ribonucleoprotein and immunizing components. J Gen Virol. 1970 Apr;7(1):19–32. doi: 10.1099/0022-1317-7-1-19. [DOI] [PubMed] [Google Scholar]
  11. Cartwright B., Smale C. J., Brown F. Surface structure of vesicular stomatitis virus. J Gen Virol. 1969 Jul;5(1):1–10. doi: 10.1099/0022-1317-5-1-1. [DOI] [PubMed] [Google Scholar]
  12. Cartwright B., Talbot P., Brown F. The proteins of biologically active sub-units of vesicular stomatitis virus. J Gen Virol. 1970 Jun;7(3):267–272. doi: 10.1099/0022-1317-7-3-267. [DOI] [PubMed] [Google Scholar]
  13. Kang C. Y., Prevec L. Proteins of vesicular stomatitis virus. I. Polyacrylamide gel analysis of viral antigens. J Virol. 1969 Apr;3(4):404–413. doi: 10.1128/jvi.3.4.404-413.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kang C. Y., Prevec L. Proteins of vesicular stomatitis virus. II. Immunological comparisons of viral antigens. J Virol. 1970 Jul;6(1):20–27. doi: 10.1128/jvi.6.1.20-27.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. McSharry J. J., Compans R. W., Choppin P. W. Proteins of vesicular stomatitis virus and of phenotypically mixed vesicular stomatitis virus-simian virus 5 virions. J Virol. 1971 Nov;8(5):722–729. doi: 10.1128/jvi.8.5.722-729.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. McSharry J. J., Wagner R. R. Lipid composition of purified vesicular stomatitis viruses. J Virol. 1971 Jan;7(1):59–70. doi: 10.1128/jvi.7.1.59-70.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mudd J. A., Summers D. F. Protein synthesis in vesicular stomatitis virus-infected HeLa cells. Virology. 1970 Oct;42(2):328–340. doi: 10.1016/0042-6822(70)90277-1. [DOI] [PubMed] [Google Scholar]
  18. Roy P., Bishop D. H. Genome homology of vesicular stomatitis virus and defective T particles and evidence for the sequential transcription of the virion ribonucleic acid. J Virol. 1972 Jun;9(6):946–955. doi: 10.1128/jvi.9.6.946-955.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Simpson R. W., Hauser R. E. Structural components of vesicular stomatitis virus. Virology. 1966 Aug;29(4):654–667. doi: 10.1016/0042-6822(66)90289-3. [DOI] [PubMed] [Google Scholar]
  20. Sokol F., Stancek D., Koprowski H. Structural proteins of rabies virus. J Virol. 1971 Feb;7(2):241–249. doi: 10.1128/jvi.7.2.241-249.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Summers D. F., Maizel J. V., Jr, Darnell J. E., Jr Evidence for virus-specific noncapsid proteins in poliovirus-infected HeLa cells. Proc Natl Acad Sci U S A. 1965 Aug;54(2):505–513. doi: 10.1073/pnas.54.2.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wagner R. R., Schnaitman T. A., Snyder R. M. Structural proteins of vesicular stomatitis viruses. J Virol. 1969 Apr;3(4):395–403. doi: 10.1128/jvi.3.4.395-403.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wagner R. R., Schnaitman T. C., Snyder R. M., Schnaitman C. A. Protein composition of the structural components of vesicular stomatitis virus. J Virol. 1969 Jun;3(6):611–618. doi: 10.1128/jvi.3.6.611-618.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

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