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. 1972 Sep;10(3):371–383. doi: 10.1128/jvi.10.3.371-383.1972

Filamentous Bacterial Viruses V. Asymmetric Replication of fd Duplex Deoxyribonucleic Acid

B Y Tseng 1, D A Marvin 1
PMCID: PMC356476  PMID: 4561204

Abstract

Short pulses (30 sec at 32 C) of 3H-thymidine were found primarily in the viral strands of replicating fd deoxyribonucleic acid (DNA), even at a time when most DNA being synthesized was duplex DNA. Much of the labeled viral strand DNA was longer than unit length, but some was shorter than unit length. Most of the corresponding complementary-strand DNA was recovered in closed supercoiled duplex molecules, even for short pulses; the remainder of the complementary-strand DNA was found in replicative intermediates in pieces shorter than unit length. Some of the viral strands in open replicating DNA lacked a corresponding complementary strand.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bourgaux P., Bourgaux-Ramoisy D., Seiler P. The replication of the ring-shaped DNA of polyoma virus. II. Identification of molecules at various stages of replication. J Mol Biol. 1971 Jul 14;59(1):195–206. doi: 10.1016/0022-2836(71)90421-9. [DOI] [PubMed] [Google Scholar]
  2. Brutlag D., Schekman R., Kornberg A. A possible role for RNA polymerase in the initiation of M13 DNA synthesis. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2826–2829. doi: 10.1073/pnas.68.11.2826. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Delius H., Howe C., Kozinski A. W. Structure of the replicating DNA from bacteriophage T4. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3049–3053. doi: 10.1073/pnas.68.12.3049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dressler D. H., Denhardt D. T. Mechanism of replication of phi-X-174 single stranded DNA. Nature. 1968 Jul 27;219(5152):346–351. doi: 10.1038/219346a0. [DOI] [PubMed] [Google Scholar]
  5. Dressler D., Wolfson J. The rolling circle for phi X DNA replication. 3. Synthesis of supercoiled duplex rings. Proc Natl Acad Sci U S A. 1970 Sep;67(1):456–463. doi: 10.1073/pnas.67.1.456. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dürwald H., Hoffmann-Berling H. Endonuclease-I-deficient and ribonuclease I-deficient Escherichia coli mutants. J Mol Biol. 1968 Jul 14;34(2):331–346. doi: 10.1016/0022-2836(68)90257-x. [DOI] [PubMed] [Google Scholar]
  7. Espejo R. T., Canelo E. S., Sinsheimer R. L. Replication of bacteriophage PM2 deoxyribonucleic acid: a closed circular double-stranded molecule. J Mol Biol. 1971 Mar 28;56(3):597–621. doi: 10.1016/0022-2836(71)90404-9. [DOI] [PubMed] [Google Scholar]
  8. Forsheit A. B., Ray D. S., Lica L. Replication of bacteriophage M13. V. Single-strand synthesis during M13 infection. J Mol Biol. 1971 Apr 14;57(1):117–127. doi: 10.1016/0022-2836(71)90122-7. [DOI] [PubMed] [Google Scholar]
  9. Freifelder D. DNA strand breakage by x-irradiation. Radiat Res. 1966 Nov;29(3):329–338. [PubMed] [Google Scholar]
  10. Gilbert W., Dressler D. DNA replication: the rolling circle model. Cold Spring Harb Symp Quant Biol. 1968;33:473–484. doi: 10.1101/sqb.1968.033.01.055. [DOI] [PubMed] [Google Scholar]
  11. Haskell E. H., Daverin C. I. Pre-fork synthesis: a model for DNA replication. Proc Natl Acad Sci U S A. 1969 Nov;64(3):1065–1071. doi: 10.1073/pnas.64.3.1065. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hohn B., von Schütz H., Marvin D. A. Filamentous bacterial viruses. II. Killing of bacteria by abortive infection with fd. J Mol Biol. 1971 Feb 28;56(1):155–165. doi: 10.1016/0022-2836(71)90091-x. [DOI] [PubMed] [Google Scholar]
  13. Inman R. B., Schnös M. Structure of branch points in replicating DNA: presence of single-stranded connections in lambda DNA branch points. J Mol Biol. 1971 Mar 14;56(2):319–325. doi: 10.1016/0022-2836(71)90467-0. [DOI] [PubMed] [Google Scholar]
  14. Jaenisch R., Mayer A., Levine A. Replicating SV40 molecules containing closed circular template DNA strands. Nat New Biol. 1971 Sep 15;233(37):72–75. doi: 10.1038/newbio233072a0. [DOI] [PubMed] [Google Scholar]
  15. Kasamatsu H., Robberson D. L., Vinograd J. A novel closed-circular mitochondrial DNA with properties of a replicating intermediate. Proc Natl Acad Sci U S A. 1971 Sep;68(9):2252–2257. doi: 10.1073/pnas.68.9.2252. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Knippers R., Whalley J. M., Sinsheimer R. L. The process of infection with bacteriophage phiX174. XXX. Replication of double-stranded phiX DNA. Proc Natl Acad Sci U S A. 1969 Sep;64(1):275–282. doi: 10.1073/pnas.64.1.275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kornberg A. Active center of DNA polymerase. Science. 1969 Mar 28;163(3874):1410–1418. doi: 10.1126/science.163.3874.1410. [DOI] [PubMed] [Google Scholar]
  18. Levine A. J., Kang H. S., Billheimer F. E. DNA replication in SV40 infected cells. I. Analysis of replicating SV40 DNA. J Mol Biol. 1970 Jun 14;50(2):549–568. doi: 10.1016/0022-2836(70)90211-1. [DOI] [PubMed] [Google Scholar]
  19. Lindqvist B. H., Sinsheimer R. L. The process of infection with bacteriophage phi-X174. XV. Bacteriophage DNA synthesis in abortive infections with a set of conditional lethal mutants. J Mol Biol. 1967 Nov 28;30(1):69–80. doi: 10.1016/0022-2836(67)90244-6. [DOI] [PubMed] [Google Scholar]
  20. Manor H., Deutscher M. P., Littauer U. Z. Rates of DNA chain growth in Escherichia coli. J Mol Biol. 1971 Nov 14;61(3):503–524. doi: 10.1016/0022-2836(71)90062-3. [DOI] [PubMed] [Google Scholar]
  21. Marvin D. A., Hohn B. Filamentous bacterial viruses. Bacteriol Rev. 1969 Jun;33(2):172–209. doi: 10.1128/br.33.2.172-209.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Marvin D. A., Schaller H. The topology of DNA from the small filamentous bacteriophage fd. J Mol Biol. 1966 Jan;15(1):1–7. doi: 10.1016/s0022-2836(66)80204-8. [DOI] [PubMed] [Google Scholar]
  23. Oishi M. Studies of DNA replication in vivo. I. Isolation of the first intermediate of DNA replication in bacteria as single-stranded DNA. Proc Natl Acad Sci U S A. 1968 May;60(1):329–336. doi: 10.1073/pnas.60.1.329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Pratt D., Erdahl W. S. Genetic control of bacteriophage M13 DNA synthesis. J Mol Biol. 1968 Oct 14;37(1):181–200. doi: 10.1016/0022-2836(68)90082-x. [DOI] [PubMed] [Google Scholar]
  25. Ray D. S. Replication of bacteriophage M13. II. The role of replicative forms in single-strand synthesis. J Mol Biol. 1969 Aug 14;43(3):631–643. doi: 10.1016/0022-2836(69)90364-7. [DOI] [PubMed] [Google Scholar]
  26. STUDIER F. W. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA. J Mol Biol. 1965 Feb;11:373–390. doi: 10.1016/s0022-2836(65)80064-x. [DOI] [PubMed] [Google Scholar]
  27. Salstrom J. S., Pratt D. Role of coliphage M13 gene 5 in single-stranded DNA production. J Mol Biol. 1971 Nov 14;61(3):489–501. doi: 10.1016/0022-2836(71)90061-1. [DOI] [PubMed] [Google Scholar]
  28. Schekman R. W., Iwaya M., Bromstrup K., Denhardt D. T. The mechanism of replication of phi X174 single-stranded DNA. 3. An enzymic study of the structure of the replicative form II DNA. J Mol Biol. 1971 Apr 28;57(2):177–199. doi: 10.1016/0022-2836(71)90340-8. [DOI] [PubMed] [Google Scholar]
  29. Schnös M., Inman R. B. Position of branch points in replicating lambda DNA. J Mol Biol. 1970 Jul 14;51(1):61–73. doi: 10.1016/0022-2836(70)90270-6. [DOI] [PubMed] [Google Scholar]
  30. Schnös M., Inman R. B. Starting point and direction of replication in P2 DNA. J Mol Biol. 1971 Jan 14;55(1):31–38. doi: 10.1016/0022-2836(71)90278-6. [DOI] [PubMed] [Google Scholar]
  31. Sebring E. D., Kelly T. J., Jr, Thoren M. M., Salzman N. P. Structure of replicating simian virus 40 deoxyribonucleic acid molecules. J Virol. 1971 Oct;8(4):478–490. doi: 10.1128/jvi.8.4.478-490.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Sinsheimer R. L. Bacteriophage phi-X174 and related viruses. Prog Nucleic Acid Res Mol Biol. 1968;8:115–169. [PubMed] [Google Scholar]
  33. Tseng B. Y., Hohn B., Marvin D. A. Filamentous bacterial viruses. IV. Fate of the infecting parental single-stranded deoxyribonucleic acid. J Virol. 1972 Sep;10(3):362–370. doi: 10.1128/jvi.10.3.362-370.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. VINOGRAD J., MORRIS J., DAVIDSON N., DOVE W. F., Jr The bouyant behavior of viral and bacterial DNA in alkaline CsCl. Proc Natl Acad Sci U S A. 1963 Jan 15;49:12–17. doi: 10.1073/pnas.49.1.12. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Yoshikawa H. The initiation of DNA replication in Bacillus subtilis. Proc Natl Acad Sci U S A. 1967 Jul;58(1):312–319. doi: 10.1073/pnas.58.1.312. [DOI] [PMC free article] [PubMed] [Google Scholar]

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