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. 1972 Oct;10(4):689–697. doi: 10.1128/jvi.10.4.689-697.1972

Transcription of the Influenza Ribonucleic Acid Genome by a Virion Polymerase III. Completeness of the Transcription Process

David H L Bishop 1, Polly Roy 1, William J Bean Jr 1, Robert W Simpson 1
PMCID: PMC356522  PMID: 5084464

Abstract

The virion ribonucleic acid (RNA) polymerase of influenza strain A0/WS transcribed at least 81% of the viral genome in vitro. The polymerase is shown to be associated with each of the major size classes of the virion RNA-ribonucleoprotein complexes. Under optimal in vitro conditions, at least 45% of the RNA contained in a population of influenza virions was involved in a repetitive transcription process. The detectable proteins associated with enzymatically active complexes containing RNA, ribonucleoprotein, and polymerase have been identified by polyacrylamide gel electrophoresis.

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Selected References

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