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. 1972 Nov;10(5):951–958. doi: 10.1128/jvi.10.5.951-958.1972

Serological Analysis of the Deoxyribonucleic Acid Polymerase of Avian Oncornaviruses I. Preparation and Characterization of Monospecific Antiserum with Purified Deoxyribonucleic Acid Polymerase

K F Watson 1,2, R C Nowinski 1,2, A Yaniv 1,2, S Spiegelman 1,2
PMCID: PMC356564  PMID: 4117965

Abstract

Monospecific antiserum was prepared against purified deoxyribonucleic acid (DNA) polymerase from avian myeloblastosis virus (AMV). Immunodiffusion assay with purified DNA polymerase revealed that the anti-DNA polymerase serum formed one precipitation band, whereas no reaction with any of the seven major structural proteins of AMV was observed. The antiserum also demonstrated enzyme-neutralizing antibody activity that was associated with the immunoglobulin G fraction. There was no difference in the neutralization of DNA polymerase activity directed by ribonucleic acid (RNA), DNA, or RNA-DNA hybrid templates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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