Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1972 Nov;10(5):985–994. doi: 10.1128/jvi.10.5.985-994.1972

Structural Proteins of Simian Virus 40: Phosphoproteins

K B Tan 1, F Sokol 1
PMCID: PMC356569  PMID: 4344258

Abstract

All five structural polypeptides of infectious simian virus 40 grown in African green monkey kidney cells were found to be phosphorylated. The polypeptides with the largest and smallest molecular weights are phosphorylated to a somewhat lower extent than the other polypeptides. The protein moiety of “empty” virus, which is essentially devoid of deoxyribonucleic acid, exhibited a degree of phosphorylation similar to that of infectious virus. In the major polypeptide (molecular weight: 49,000), the phosphate appears to be bound to the seryl or threonyl residues, or both. The nature of the phosphate-polypeptide bond in the other viral polypeptides remains obscure.

Full text

PDF
990

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLACK P. H., CRAWFORD E. M., CRAWFORD L. V. THE PURIFICATION OF SIMIAN VIRUS 40. Virology. 1964 Nov;24:381–387. doi: 10.1016/0042-6822(64)90175-8. [DOI] [PubMed] [Google Scholar]
  2. Carp R. I., Sauer G., Sokol F. The effect of actinomycin D on the transcription and replication of simian virus 40 deoxyribonucleic acid. Virology. 1969 Feb;37(2):214–226. doi: 10.1016/0042-6822(69)90201-3. [DOI] [PubMed] [Google Scholar]
  3. Frearson P. M., Crawford L. V. Polyoma virus basic proteins. J Gen Virol. 1972 Feb;14(2):141–155. doi: 10.1099/0022-1317-14-2-141. [DOI] [PubMed] [Google Scholar]
  4. Hatanaka M., Twiddy E., Gilden R. V. Protein kinase associated with RNA tumor viruses and other budding RNA viruses. Virology. 1972 Feb;47(2):536–538. doi: 10.1016/0042-6822(72)90297-8. [DOI] [PubMed] [Google Scholar]
  5. Hokin L. E., Sastry P. S., Galsworthy P. R., Yoda A. Evidence that a phosphorylated intermediate in a brain transport adenosine triphosphatase is an acyl phosphate. Proc Natl Acad Sci U S A. 1965 Jul;54(1):177–184. doi: 10.1073/pnas.54.1.177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hultquist D. E., Moyer R. W., Boyer P. D. The preparation and characterization of 1-phosphohistidine and 3-phosphohistidine. Biochemistry. 1966 Jan;5(1):322–331. doi: 10.1021/bi00865a041. [DOI] [PubMed] [Google Scholar]
  7. Ingles C. J., Dixon G. H. Phosphorylation of protamine during spermatogenesis in trout testis. Proc Natl Acad Sci U S A. 1967 Sep;58(3):1011–1018. doi: 10.1073/pnas.58.3.1011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. JENSEN F. C., GIRARDI A. J., GILDEN R. V., KOPROWSKI H. INFECTION OF HUMAN AND SIMIAN TISSUE CULTURES WITH ROUS SARCOMA VIRUS. Proc Natl Acad Sci U S A. 1964 Jul;52:53–59. doi: 10.1073/pnas.52.1.53. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kleinsmith L. J., Allfrey V. G., Mirsky A. E. Phosphorylation of nuclear protein early in the course of gene activation in lymphocytes. Science. 1966 Nov 11;154(3750):780–781. doi: 10.1126/science.154.3750.780. [DOI] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. PERLMANN G. E. The nature of phosphorus linkages in phosphoproteins. Adv Protein Chem. 1955;10:1–30. doi: 10.1016/s0065-3233(08)60103-5. [DOI] [PubMed] [Google Scholar]
  12. Randall C. C., Rogers H. W., Downer D. N., Gentry G. A. Protein kinase activity in equine herpesvirus. J Virol. 1972 Feb;9(2):216–222. doi: 10.1128/jvi.9.2.216-222.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Roblin R., Härle E., Dulbecco R. Polyoma virus proteins. 1. Multiple virion components. Virology. 1971 Sep;45(3):555–566. doi: 10.1016/0042-6822(71)90171-1. [DOI] [PubMed] [Google Scholar]
  14. Sokol F., Kuwert E., Wiktor T. J., Hummeler K., Koprowski H. Purification of rabies virus grown in tissue culture. J Virol. 1968 Aug;2(8):836–849. doi: 10.1128/jvi.2.8.836-849.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sokol F., Stancek D., Koprowski H. Structural proteins of rabies virus. J Virol. 1971 Feb;7(2):241–249. doi: 10.1128/jvi.7.2.241-249.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Strand M., August J. T. Protein kinase and phosphate acceptor proteins in Rauscher murine leukaemia virus. Nat New Biol. 1971 Sep 29;233(39):137–140. doi: 10.1038/newbio233137a0. [DOI] [PubMed] [Google Scholar]
  17. Tan K. B. A simple device for fractionating density gradients. Anal Biochem. 1972 Jan;45(1):306–308. doi: 10.1016/0003-2697(72)90031-0. [DOI] [PubMed] [Google Scholar]
  18. Tan K. B., McAuslan B. R. Proteins of polyhedral cytoplasmic deoxyviruses. I. The structural polypeptides of FV 3 . Virology. 1971 Jul;45(1):200–207. doi: 10.1016/0042-6822(71)90127-9. [DOI] [PubMed] [Google Scholar]
  19. Wand M., Zeuthen E., Evans E. A. Tritiated thymidine: effect of decomposition by self-radiolysis on specificity as a tracer for DNA synthesis. Science. 1967 Jul 28;157(3787):436–438. doi: 10.1126/science.157.3787.436. [DOI] [PubMed] [Google Scholar]
  20. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]
  21. Zeigel R. F., Clark H. F. Electron microscopic observations on a "C"-type virus in cell cultures derived from a tumor-bearing viper. J Natl Cancer Inst. 1969 Nov;43(5):1097–1102. [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES