Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1973 Aug;12(2):320–327. doi: 10.1128/jvi.12.2.320-327.1973

Membrane Proteins of Uninfected and Rous Sarcoma Virus-Transformed Avian Cells

Robert H Bussell a,1, William S Robinson a
PMCID: PMC356626  PMID: 4355934

Abstract

A method for preparing large membrane fragments and cell ghosts was developed for uninfected and Rous sarcoma virus-transformed chicken embryo fibroblasts in culture. Membrane proteins were analyzed by electrophoresis in acrylamide gels containing sodium dodecyl sulfate. A major amino-acid-containing component of uninfected cell membranes was greatly diminished in amount or absent in membranes of virus-transformed cells. This component, called MP-1, had an electrophoretic mobility in sodium dodecyl sulfate-containing gels similar to that of a protein of a mol wt of 1.42 × 105. MP-1 was not altered by changes in cell growth rate or in cells infected with the nontransforming virus RAV-1.

Full text

PDF
321

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atkinson P. H., Summers D. F. Purification and properties of HeLa cell plasma membranes. J Biol Chem. 1971 Aug 25;246(16):5162–5175. [PubMed] [Google Scholar]
  2. Buck C. A., Glick M. C., Warren L. A comparative study of glycoproteins from the surface of control and Rous sarcoma virus transformed hamster cells. Biochemistry. 1970 Nov 10;9(23):4567–4576. doi: 10.1021/bi00825a016. [DOI] [PubMed] [Google Scholar]
  3. Buck C. A., Glick M. C., Warren L. Glycopeptides from the surface of control and virus-transformed cells. Science. 1971 Apr 9;172(3979):169–171. doi: 10.1126/science.172.3979.169. [DOI] [PubMed] [Google Scholar]
  4. Burger M. M. A difference in the architecture of the surface membrane of normal and virally transformed cells. Proc Natl Acad Sci U S A. 1969 Mar;62(3):994–1001. doi: 10.1073/pnas.62.3.994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Burger M. M., Goldberg A. R. Identification of a tumor-specific determinant on neoplastic cell surfaces. Proc Natl Acad Sci U S A. 1967 Feb;57(2):359–366. doi: 10.1073/pnas.57.2.359. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Burger M. M., Martin G. S. Agglutination of cells transformed by Rous sarcoma virus by wheat germ agglutinin and concanavalin A. Nat New Biol. 1972 May 3;237(70):9–12. doi: 10.1038/newbio237009a0. [DOI] [PubMed] [Google Scholar]
  7. Chiarugi V. P., Urbano P. Electrophoretic analysis of membrane glycoproteins in normal and polyoma virus transformed BHK 21 cells. J Gen Virol. 1972 Feb;14(2):133–140. doi: 10.1099/0022-1317-14-2-133. [DOI] [PubMed] [Google Scholar]
  8. Cohen G. H., Atkinson P. H., Summers D. F. Interactions of vesicular stomatitis virus structural proteins with HeLa plasma membranes. Nat New Biol. 1971 May 26;231(21):121–123. doi: 10.1038/newbio231121a0. [DOI] [PubMed] [Google Scholar]
  9. David A. E. Lipid composition of Sindbis virus. Virology. 1971 Dec;46(3):711–720. doi: 10.1016/0042-6822(71)90073-0. [DOI] [PubMed] [Google Scholar]
  10. FORRESTER J. A., AMBROSE E. J., MACPHERSON J. A. Electrophoretic investigations of a clone of hamster fibroblasts and polyoma-transformed cells from the same population. Nature. 1962 Dec 15;196:1068–1070. doi: 10.1038/1961068a0. [DOI] [PubMed] [Google Scholar]
  11. FORRESTER J. A., AMBROSE E. J., STOKER M. G. MICROELECTROPHORESIS OF NORMAL AND TRANSFORMED CLONES OF HAMSTER KIDNEY FIBROBLASTS. Nature. 1964 Feb 29;201:945–946. doi: 10.1038/201945a0. [DOI] [PubMed] [Google Scholar]
  12. Hakomori S. I., Murakami W. T. Glycolipids of hamster fibroblasts and derived malignant-transformed cell lines. Proc Natl Acad Sci U S A. 1968 Jan;59(1):254–261. doi: 10.1073/pnas.59.1.254. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hatanaka M., Hanafusa H. Analysis of a functional change in membrane in the process of cell transformation by Rous sarcoma virus; alteration in the characteristics of sugar transport. Virology. 1970 Aug;41(4):647–652. doi: 10.1016/0042-6822(70)90429-0. [DOI] [PubMed] [Google Scholar]
  14. Hatanaka M., Huebner R. J., Gilden R. V. Alterations in the characteristics of sugar uptake by mouse cells transformed by murine sarcoma viruses. J Natl Cancer Inst. 1969 Nov;43(5):1091–1096. [PubMed] [Google Scholar]
  15. Hobom-Schnegg B., Robinson H. L., Robinson W. S. Replication of Rous sarcoma virus in synchronized cells. J Gen Virol. 1970;7(2):85–93. doi: 10.1099/0022-1317-7-2-85. [DOI] [PubMed] [Google Scholar]
  16. Holland J. J., Kiehn E. D. Influenza virus effects on cell membrane proteins. Science. 1970 Jan 9;167(3915):202–205. doi: 10.1126/science.167.3915.202. [DOI] [PubMed] [Google Scholar]
  17. Hung P. P., Robinson H. L., Robinson W. S. Isolation and characterization of proteins from Rous sarcoma virus. Virology. 1971 Jan;43(1):251–266. doi: 10.1016/0042-6822(71)90243-1. [DOI] [PubMed] [Google Scholar]
  18. Inbar M., Sachs L. Interaction of the carbohydrate-binding protein concanavalin A with normal and transformed cells. Proc Natl Acad Sci U S A. 1969 Aug;63(4):1418–1425. doi: 10.1073/pnas.63.4.1418. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Lazarowitz S. G., Compans R. W., Choppin P. W. Influenza virus structural and nonstructural proteins in infected cells and their plasma membranes. Virology. 1971 Dec;46(3):830–843. doi: 10.1016/0042-6822(71)90084-5. [DOI] [PubMed] [Google Scholar]
  20. Martin G. S., Venuta S., Weber M., Rubin H. Temperature-dependent alterations in sugar transport in cells infected by a temperature-sensitive mutant of Rous sarcoma virus. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2739–2741. doi: 10.1073/pnas.68.11.2739. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Meezan E., Wu H. C., Black P. H., Robbins P. W. Comparative studies on the carbohydrate-containing membrane components of normal and virus-transformed mouse fibroblasts. II. Separation of glycoproteins and glycopeptides by sephadex chromatography. Biochemistry. 1969 Jun;8(6):2518–2524. doi: 10.1021/bi00834a039. [DOI] [PubMed] [Google Scholar]
  22. Mora P. T., Brady R. O., Bradley R. M., McFarland V. W. Gangliosides in DNA virus-transformed and spontaneously transformed tumorigenic mouse cell lines. Proc Natl Acad Sci U S A. 1969 Aug;63(4):1290–1296. doi: 10.1073/pnas.63.4.1290. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Perdue J. F., Kletzien R., Miller K. The isolation and characterization of plasma membrane from cultured cells. I. The chemical composition of membrane isolated from uninfected and oncogenic RNA virus-converted chick embryo fibroblasts. Biochim Biophys Acta. 1971 Dec 3;249(2):419–434. doi: 10.1016/0005-2736(71)90120-9. [DOI] [PubMed] [Google Scholar]
  24. Perdue J. F., Kletzien R., Wray V. L. The isolation and characterization of plasma membrane from cultured cells. IV. The carbohydrate composition of membranes isolated from oncogenic RNA virus-converted chick embryo fibroblasts. Biochim Biophys Acta. 1972 May 9;266(2):505–510. doi: 10.1016/0005-2736(72)90106-x. [DOI] [PubMed] [Google Scholar]
  25. RUBIN H., VOGT P. K. An avian leukosis virus associated with stocks of Rous sarcoma virus. Virology. 1962 May;17:184–194. doi: 10.1016/0042-6822(62)90096-x. [DOI] [PubMed] [Google Scholar]
  26. Robinson H. L., Robinson W. S. Inhibition of growth of uninfected and rous sarcoma virus-infected chick-embryo fibroblasts by rifampicin. J Natl Cancer Inst. 1971 Apr;46(4):785–788. [PubMed] [Google Scholar]
  27. Shapiro A. L., Viñuela E., Maizel J. V., Jr Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochem Biophys Res Commun. 1967 Sep 7;28(5):815–820. doi: 10.1016/0006-291x(67)90391-9. [DOI] [PubMed] [Google Scholar]
  28. Stoker M. G., Rubin H. Density dependent inhibition of cell growth in culture. Nature. 1967 Jul 8;215(5097):171–172. doi: 10.1038/215171a0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES