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. 1973 Oct;12(4):841–846. doi: 10.1128/jvi.12.4.841-846.1973

Deoxyribonucleic Acid of Marek's Disease Virus in Virus-Induced Tumors

K Nazerian a,1, T Lindahl a, G Klein a, L F Lee a,1
PMCID: PMC356702  PMID: 4591050

Abstract

DNA was extracted from [3H]thymidine-labeled Marek's disease virus (MDV) and purified by two cycles of CsCl gradient centrifugation in a fixed-angle rotor. The DNA was transcribed in vitro into 32P-labeled complementary RNA (cRNA). MDV cRNA did not hybridize with DNA from chicken embryo fibroblast cultures or from chicken spleen, but hybridized efficiently with DNA from MDV particles or MDV-infected cell cultures. Five Marek's disease tumors from different chickens and different organs (ovary, liver, testis) were all found to contain MDV DNA sequences. The relative amount of MDV DNA varied from tumor to tumor and was between 3 and 15 virus genome equivalents per cell. The content of virus DNA per cell in spleens from tumor-bearing chickens was much lower than in tumors from the same animals. MDV-infected cell cultures contained a large proportion (28-59%) of virus antigen-positive cells, as measured by immunofluorescence, but tumor cells were negative in this respect (<0.02% positive cells). These data indicate that MDV is present in a provirus form in tumor cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atkin N. B., Mattinson G., Beçak W., Ohno S. The comparative DNA content of 19 species of placental mammals, reptiles and birds. Chromosoma. 1965;17(1):1–10. doi: 10.1007/BF00285152. [DOI] [PubMed] [Google Scholar]
  2. Birnstiel M. L., Sells B. H., Purdom I. F. Kinetic complexity of RNA molecules. J Mol Biol. 1972 Jan 14;63(1):21–39. doi: 10.1016/0022-2836(72)90519-0. [DOI] [PubMed] [Google Scholar]
  3. Calnek B. W., Adldinger H. K., Kahn D. E. Feather follicle epithelium: a source of enveloped and infectious cell-free herpesvirus from Marek's disease. Avian Dis. 1970 May;14(2):219–233. [PubMed] [Google Scholar]
  4. Doerfler W. Integration of the deoxyribonucleic acid of adenovirus type 12 into the deoxyribonucleic acid of baby hamster kidney cells. J Virol. 1970 Nov;6(5):652–666. doi: 10.1128/jvi.6.5.652-666.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Eidson C. S., Schmittle S. C. Studies on acute Marek's disease. I. Characteristics of isolate GA in chickens. Avian Dis. 1968 Aug;12(3):467–476. [PubMed] [Google Scholar]
  6. Gergely L., Klein G., Ernberg I. Host cell macromolecular synthesis in cells containing EBV-induced early antigens, studied by combined immunofluorescence and radioautography. Virology. 1971 Jul;45(1):22–29. doi: 10.1016/0042-6822(71)90108-5. [DOI] [PubMed] [Google Scholar]
  7. Haas M., Vogt M., Dulbecco R. Loss of simian virus 40 DNA-RNA hybrids from nitrocellulose membranes; implications for the study of virus--host DNA interactions. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2160–2164. doi: 10.1073/pnas.69.8.2160. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jehn U., Lindahl T., Klein C. Fate of virus DNA in the abortive infection of human lymphoid cell lines by Epstein-Barr virus. J Gen Virol. 1972 Sep;16(3):409–412. doi: 10.1099/0022-1317-16-3-409. [DOI] [PubMed] [Google Scholar]
  9. Lee L. F., Armstrong R. L., Nazerian K. Comparative studies of six avian herpesviruses. Avian Dis. 1972 Jul-Sep;16(4):799–805. [PubMed] [Google Scholar]
  10. Lee L. F. Induction of deoxyribonucleic acid synthesis and the oncogenicity of Marek's disease virus. J Virol. 1972 Aug;10(2):167–170. doi: 10.1128/jvi.10.2.167-170.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lee L. F., Kieff E. D., Bachenheimer S. L., Roizman B., Spear P. G., Burmester B. R., Nazerian K. Size and composition of Marek's disease virus deoxyribonucleic acid. J Virol. 1971 Mar;7(3):289–294. doi: 10.1128/jvi.7.3.289-294.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nazerian K. Attenuation of Marek's disease virus and study of its properties in two different cell cultures. J Natl Cancer Inst. 1970 Jun;44(6):1257–1267. [PubMed] [Google Scholar]
  13. Nazerian K., Burmester B. R. Electron microscopy of a herpes virus associated with the agent of Marek's disease in cell culture. Cancer Res. 1968 Dec;28(12):2454–2462. [PubMed] [Google Scholar]
  14. Nazerian K., Witter R. L. Cell-free transmission and in vivo replication of Marek's disease virus. J Virol. 1970 Mar;5(3):388–397. doi: 10.1128/jvi.5.3.388-397.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Nonoyama M., Pagano J. S. Detection of Epstein-Barr viral genome in nonproductive cells. Nat New Biol. 1971 Sep 22;233(38):103–106. doi: 10.1038/newbio233103a0. [DOI] [PubMed] [Google Scholar]
  16. Nonoyama M., Pagano J. S. Homology between Epstein-Barr virus DNA and viral DNA from Burkitt's lymphoma and nasopharyngeal carcinoma determined by DNA-DNA reassociation kinetics. Nature. 1973 Mar 2;242(5392):44–47. doi: 10.1038/242044a0. [DOI] [PubMed] [Google Scholar]
  17. Okazaki W., Purchase H. G., Burmester B. R. Protection against Marek's disease by vaccination with a herpesvirus of turkeys. Avian Dis. 1970 May;14(2):413–429. [PubMed] [Google Scholar]
  18. Pettersson U., Sambrook J. Amount of viral DNA in the genome of cells transformed by adenovirus type 2. J Mol Biol. 1973 Jan;73(1):125–130. doi: 10.1016/0022-2836(73)90164-2. [DOI] [PubMed] [Google Scholar]
  19. Purchase H. G., Burmester B. R., Cunningham C. H. Pathogenicity and Antigenicity of Clones from Strains of Marek's Disease Virus and the Herpesvirus of Turkeys. Infect Immun. 1971 Feb;3(2):295–303. doi: 10.1128/iai.3.2.295-303.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Purchase H. G. Immunofluorescence in the study of Marek's disease. I. Detection of antigen in cell culture and an antigenic comparison of eight isolates. J Virol. 1969 Jun;3(6):557–565. doi: 10.1128/jvi.3.6.557-565.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Westphal H., Dulbecco R. Viral DNA in polyoma- and SV40-transformed cell lines. Proc Natl Acad Sci U S A. 1968 Apr;59(4):1158–1165. doi: 10.1073/pnas.59.4.1158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Witter R. L., Nazerian K., Purchase H. G., Burgoyne G. H. Isolation from turkeys of a cell-associated herpesvirus antigenically related to Marek's disease virus. Am J Vet Res. 1970 Mar;31(3):525–538. [PubMed] [Google Scholar]
  23. Zur Hausen H., Schulte-Holthausen H. Presence of EB virus nucleic acid homology in a "virus-free" line of Burkitt tumour cells. Nature. 1970 Jul 18;227(5255):245–248. doi: 10.1038/227245a0. [DOI] [PubMed] [Google Scholar]
  24. zur Hausen H., Schulte-Holthausen H., Klein G., Henle W., Henle G., Clifford P., Santesson L. EBV DNA in biopsies of Burkitt tumours and anaplastic carcinomas of the nasopharynx. Nature. 1970 Dec 12;228(5276):1056–1058. doi: 10.1038/2281056a0. [DOI] [PubMed] [Google Scholar]

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