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. 1973 Oct;12(4):919–930. doi: 10.1128/jvi.12.4.919-930.1973

Herpes Simplex Virus and Human Cytomegalovirus Replication in WI-38 Cells I. Sequence of Viral Replication

Janet Duyckinck Smith a,1, Etienne de Harven a
PMCID: PMC356711  PMID: 4359959

Abstract

A comparison, under standardized conditions, of herpes simplex virus (HSV) and human cytomegalovirus (CMV) revealed differences in viral morphology, in the timing of their infectious cycles, and in several morphological events during those cycles. Structural distinctions between the two viruses included the coating of unenveloped cytoplasmic CMV capsids, but not those of HSV, and a variation in the structure of their cores. Since the two cycles were carried out in the same host cell strain under conditions of one-step growth (input multiplicity = 10 PFU/cell), it was possible to construct time scales locating the major events of each cycle. Comparison of the two showed that HSV replicated and released progeny within 8 h postinfection, whereas CMV required 4 days. These results correlated well with those of concurrent plaque assays. Within the longer CMV cycle, most of the major events appeared retarded to a similar degree, and no obvious limiting step in particle production could be identified. Distinctions between the two cycles included the following: condensation of the chromatin in HSV- but not CMV-infected cells; the greater tendency of HSV to produce membrane alterations; and the appearance of cytoplasmic dense bodies in CMV- but not HSV-infected cells. Identification of these differences even under identical conditions of culture and infection strongly implies that they result from intrinsic differences in the nature of the viruses, and are not caused by variations in experimental conditions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BEALE A. J., HAIR H. C. Rapid diagnosis of the herpetic variety of kaposi's varicelliform eruption by tissue culture methods. Can Med Assoc J. 1956 Mar 15;74(6):443–448. [PMC free article] [PubMed] [Google Scholar]
  2. BLANK H., BURGOON C. F., BALDRIDGE G. D., McCARTHY P. L., URBACH F. Cytologic smears in diagnosis of herpes simplex, herpes zoster, and varicella. J Am Med Assoc. 1951 Aug 11;146(15):1410–1412. doi: 10.1001/jama.1951.63670150005012b. [DOI] [PubMed] [Google Scholar]
  3. Brinkley B. R., Murphy P., Richardson L. C. Procedure for embedding in situ selected cells cultured in vitro. J Cell Biol. 1967 Oct;35(1):279–283. doi: 10.1083/jcb.35.1.279. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chambers R. W., Rose J. A., Rabson A. S., Bond H. E., Hall W. T. Propagation and purification of high-titer human cytomegalovirus. Appl Microbiol. 1971 Nov;22(5):914–918. doi: 10.1128/am.22.5.914-918.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DE HARVEN E. VIREMIA IN FRIEND MURINE LEUKEMIA: THE ELECTRON MICROSCOPE APPROACH TO THE PROBLEM. Pathol Biol. 1965 Feb;13:125–134. [PubMed] [Google Scholar]
  6. DOANE F., RHODES A. J., ORMSBY H. L. Tissue culture techniques in the study of herpetic infections of the eye. Am J Ophthalmol. 1955 Nov;40(5 Pt 2):189–193. [PubMed] [Google Scholar]
  7. Furlong D., Swift H., Roizman B. Arrangement of herpesvirus deoxyribonucleic acid in the core. J Virol. 1972 Nov;10(5):1071–1074. doi: 10.1128/jvi.10.5.1071-1074.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Iwasaki Y., Furukawa T., Plotkin S., Koprowski H. Ultrastructural study on the sequence of human cytomegalovirus infection in human diploid cells. Arch Gesamte Virusforsch. 1973;40(3):311–324. doi: 10.1007/BF01242551. [DOI] [PubMed] [Google Scholar]
  9. Kanich R. E., Craighead J. E. Human cytomegalovirus infection of cultured fibroblasts. II. Viral replicative sequence of a wild and an adapted strain. Lab Invest. 1972 Sep;27(3):273–282. [PubMed] [Google Scholar]
  10. MCGAVRAN M. H., SMITH M. G. ULTRASTRUCTURAL, CYTOCHEMICAL, AND MICROCHEMICAL OBSERVATIONS ON CYTOMEGALOVIRUS (SALIVARY GLAND VIRUS) INFECTION OF HUMAN CELLS IN TISSUE CULTURE. Exp Mol Pathol. 1965 Feb;76:1–10. doi: 10.1016/0014-4800(65)90019-5. [DOI] [PubMed] [Google Scholar]
  11. MORGAN C., ROSE H. M., HOLDEN M., JONES E. P. Electron microscopic observations on the development of herpes simplex virus. J Exp Med. 1959 Oct 1;110:643–656. doi: 10.1084/jem.110.4.643. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nii S., Morgan C., Rose H. M. Electron microscopy of herpes simplex virus. II. Sequence of development. J Virol. 1968 May;2(5):517–536. doi: 10.1128/jvi.2.5.517-536.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. RUEBNER B. H., HIRANO T., SLUSSER R. J., MEDEARIS D. N., Jr HUMAN CYTOMEGALOVIRUS INFECTION. ELECTRON MICROSCOPIC AND HISTOCHEMICAL CHANGES IN CULTURES OF HUMAN FIBROBLASTS. Am J Pathol. 1965 Mar;46:477–496. [PMC free article] [PubMed] [Google Scholar]
  14. SCOTT T. F., McLEOD D. L. Cellular responses to infection with strains of herpes simplex virus. Ann N Y Acad Sci. 1959 Jul 21;81:118–128. doi: 10.1111/j.1749-6632.1959.tb49300.x. [DOI] [PubMed] [Google Scholar]
  15. SMITH K. O., RASMUSSEN L. MORPHOLOGY OF CYTOMEGALOVIRUS (SALIVARY GLAND VIRUS). J Bacteriol. 1963 Jun;85:1319–1325. doi: 10.1128/jb.85.6.1319-1325.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Schwartz J., Roizman B. Concerning the egress of herpes simplex virus from infected cells: electron and light microscope observations. Virology. 1969 May;38(1):42–49. doi: 10.1016/0042-6822(69)90126-3. [DOI] [PubMed] [Google Scholar]
  17. Shipkey F. H., Erlandson R. A., Bailey R. B., Babcock V. I., Southam C. M. Virus biographies. II. Growth of herpes simplex virus in tissue culture. Exp Mol Pathol. 1967 Feb;6(1):39–67. doi: 10.1016/0014-4800(67)90005-6. [DOI] [PubMed] [Google Scholar]
  18. Siminoff P., Menefee M. G. Normal and 5-bromodeoxyuridine-inhibited development of herpes simplex virus. An electron microscope study. Exp Cell Res. 1966 Nov-Dec;44(2):241–255. doi: 10.1016/0014-4827(66)90429-0. [DOI] [PubMed] [Google Scholar]
  19. Smith J. D., De Harven E. Concentration of herpesviruses. J Virol. 1973 Feb;11(2):325–328. doi: 10.1128/jvi.11.2.325-328.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Stackpole C. W. Herpes-type virus of the frog renal adenocarcinoma. I. Virus development in tumor transplants maintained at low temperature. J Virol. 1969 Jul;4(1):75–93. doi: 10.1128/jvi.4.1.75-93.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. WELLER T. H., HANSHAW J. B., SCOTT D. E. Serologic differentiation of viruses responsible for cytomegalic inclusion disease. Virology. 1960 Sep;12:130–132. doi: 10.1016/0042-6822(60)90156-2. [DOI] [PubMed] [Google Scholar]
  22. WILDY P., RUSSELL W. C., HORNE R. W. The morphology of herpes virus. Virology. 1960 Oct;12:204–222. doi: 10.1016/0042-6822(60)90195-1. [DOI] [PubMed] [Google Scholar]
  23. Wentworth B. B., French L. Plaque assay of Herpesvirus hominis on human embryonic fibroblasts. Proc Soc Exp Biol Med. 1969 Jun;131(2):588–592. doi: 10.3181/00379727-131-33932. [DOI] [PubMed] [Google Scholar]
  24. Wentworth B. B., French L. Plaque assay of cytomegalovirus strains of human origin. Proc Soc Exp Biol Med. 1970 Nov;135(2):253–258. doi: 10.3181/00379727-135-35031. [DOI] [PubMed] [Google Scholar]

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