Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1973 Nov;12(5):1131–1138. doi: 10.1128/jvi.12.5.1131-1138.1973

Studies on the Mechanism of Replication of Adenovirus DNA II. The Nature of Single-Stranded DNA in Replicative Intermediates

J S Sussenbach a, D J Ellens a,1, H S Jansz a
PMCID: PMC356745  PMID: 4765398

Abstract

Replicative intermediates of adenovirus type 5 DNA contain large stretches of single-stranded DNA. We have shown that this single-stranded DNA is mainly of parental origin, whereas all new DNA synthesized during one round of replication has a double-stranded structure. Hybridization experiments of the single-stranded DNA with isolated complementary strands of adenovirus type 5 DNA showed that this DNA hybridized only with the viral L-strand (the strand with the lower equilibrium density in alkaline CsCl) indicating that it represents the viral H-strand. This observation implies that replication always starts from one and the same molecular end. Electron microscopy of partially denatured Y-shaped intermediates confirmed this and showed that replication started from the molecular right end (the end richest in A-T base pairs). In conclusion, we have shown that replication of adenovirus type 5 DNA starts at the molecular right end, displacing the parental H-strand.

Full text

PDF
1132

Images in this article

1136Image
on p.1136

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aloni Y., Winocour E., Sachs L., Torten J. Hybridization between SV40 DNA and cellular DNA's. J Mol Biol. 1969 Sep 14;44(2):333–345. doi: 10.1016/0022-2836(69)90179-x. [DOI] [PubMed] [Google Scholar]
  2. Bellett A. J., Younghusband H. B. Replication of the DNA of chick embryo lethal orphan virus. J Mol Biol. 1972 Dec 30;72(3):691–709. doi: 10.1016/0022-2836(72)90185-4. [DOI] [PubMed] [Google Scholar]
  3. Doerfler W., Kleinschmidt A. K. Denaturation pattern of the DNA of adenovirus type 2 as determined by electron microscopy. J Mol Biol. 1970 Jun 28;50(3):579–593. doi: 10.1016/0022-2836(70)90086-0. [DOI] [PubMed] [Google Scholar]
  4. Flavell R. A., Borst P., ter Schegget J. DNA synthesis by isolated mitochondria. IV. Isolation of an intermediate containing newly synthesized DNA in full-length light strands. Biochim Biophys Acta. 1972 Jul 20;272(3):341–349. [PubMed] [Google Scholar]
  5. Kiger J. A., Jr, Sinsheimer R. L. Vegetative lambda DNA. IV. Fractionation of replicating lambda DNA on benzoylated-naphthoylated DEAE cellulose. J Mol Biol. 1969 Mar 28;40(3):467–490. doi: 10.1016/0022-2836(69)90166-1. [DOI] [PubMed] [Google Scholar]
  6. Sussenbach J. S., van der Vliet P. C., Ellens D. J., Jansz H. S. Linear intermediates in the replication of adenovirus DNA. Nat New Biol. 1972 Sep 13;239(89):47–49. [PubMed] [Google Scholar]
  7. Sussenbach J. S., van der Vliet P. C. Studies on the mechanism of replication of adenovirus DNA. I. The effect of hydroxyurea. Virology. 1973 Jul;54(1):299–303. doi: 10.1016/0042-6822(73)90142-6. [DOI] [PubMed] [Google Scholar]
  8. Sussenbach J. S., van der Vliet P. C. Viral DNA synthesis in isolated nuclei from adenovirus-infected KB cells. FEBS Lett. 1972 Mar;21(1):7–10. doi: 10.1016/0014-5793(72)80149-2. [DOI] [PubMed] [Google Scholar]
  9. van der Eb A. J. Intermediates in type 5 adenovirus DNA replication. Virology. 1973 Jan;51(1):11–23. doi: 10.1016/0042-6822(73)90361-9. [DOI] [PubMed] [Google Scholar]
  10. van der Vliet P. C., Sussenbach J. S. The mechanism of adenovirus-DNA synthesis in isolated nuclei. Eur J Biochem. 1972 Nov 7;30(3):584–592. doi: 10.1111/j.1432-1033.1972.tb02130.x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES