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. 1989 Apr;2(2):158–165. doi: 10.1128/cmr.2.2.158

Microsporidia and human infections.

J A Shadduck 1, E Greeley 1
PMCID: PMC358108  PMID: 2650860

Abstract

Protozoa of the phylum Microspora are obligate intracellular pathogens that are being detected with increasing frequency in humans, especially in patients with acquired immunodeficiency syndrome. Organisms from four genera have been reported to date, and serological data suggest the occurrence of latent infections. Sources of human infections are not known, but microsporidia are widespread in lower vertebrates and invertebrates. There is no known treatment. Study of the disease in mammals suggests that infection often will be clinically silent, that intact T-cell-mediated host defenses are required for resistance, and that serious clinical disease may occur under circumstances in which extensive parasite replication can occur.

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Selected References

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  1. Arison R. N., Cassaro J. A., Pruss M. P. Studies on a murine ascites-producing agent and its effect on tumor development. Cancer Res. 1966 Sep;26(9):1915–1920. [PubMed] [Google Scholar]
  2. Ashton N., Wirasinha P. A. Encephalitozoonosis (nosematosis) of the cornea. Br J Ophthalmol. 1973 Sep;57(9):669–674. doi: 10.1136/bjo.57.9.669. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Barker R. J. The nature of Encephalitozoon brumpti Coulon, 1924. J Parasitol. 1974 Jun;60(3):542–544. [PubMed] [Google Scholar]
  4. Beckwith C., Peterson N., Liu J. J., Shadduck J. A. Dot enzyme-linked immunosorbent assay (dot ELISA) for antibodies to Encephalitozoon cuniculi. Lab Anim Sci. 1988 Oct;38(5):573–576. [PubMed] [Google Scholar]
  5. Bergquist N. R., Stintzing G., Smedman L., Waller T., Andersson T. Diagnosis of encephalitozoonosis in man by serological tests. Br Med J (Clin Res Ed) 1984 Mar 24;288(6421):902–902. doi: 10.1136/bmj.288.6421.902. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bergquist R., Morfeldt-Månsson L., Pehrson P. O., Petrini B., Wasserman J. Antibody against Encephalitozoon cuniculi in Swedish homosexual men. Scand J Infect Dis. 1984;16(4):389–391. doi: 10.3109/00365548409073966. [DOI] [PubMed] [Google Scholar]
  7. Bismanis J. E. Detection of latent murine nosematosis and growth of Nosema cuniculi in cell cultures. Can J Microbiol. 1970 Apr;16(4):237–242. doi: 10.1139/m70-044. [DOI] [PubMed] [Google Scholar]
  8. Botha W. S., van Dellen A. F., Stewart C. G. Canine encephalitozoonosis in South Africa. J S Afr Vet Assoc. 1979 Jun;50(2):135–144. [PubMed] [Google Scholar]
  9. Bywater J. E. Is encephalitozoonosis a zoonosis? Lab Anim. 1979 Apr;13(2):149–151. doi: 10.1258/002367779780943440. [DOI] [PubMed] [Google Scholar]
  10. Canning E. U., Hollister W. S. Microsporidia of mammals--widespread pathogens or opportunistic curiosities? Parasitol Today. 1987 Sep;3(9):267–273. doi: 10.1016/0169-4758(87)90103-7. [DOI] [PubMed] [Google Scholar]
  11. Cox J. C., Gallichio H. A., Pye D., Walden N. B. Application of immunofluorescence to the establishment of an Encephalitozoon cuniculi-free rabbit colony. Lab Anim Sci. 1977 Apr;27(2):204–209. [PubMed] [Google Scholar]
  12. Curry A., McWilliam L. J., Haboubi N. Y., Mandal B. K. Microsporidiosis in a British patient with AIDS. J Clin Pathol. 1988 Apr;41(4):477–478. doi: 10.1136/jcp.41.4.477-b. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Desportes I., Le Charpentier Y., Galian A., Bernard F., Cochand-Priollet B., Lavergne A., Ravisse P., Modigliani R. Occurrence of a new microsporidan: Enterocytozoon bieneusi n.g., n. sp., in the enterocytes of a human patient with AIDS. J Protozool. 1985 May;32(2):250–254. doi: 10.1111/j.1550-7408.1985.tb03046.x. [DOI] [PubMed] [Google Scholar]
  14. Dobbins W. O., 3rd, Weinstein W. M. Electron microscopy of the intestine and rectum in acquired immunodeficiency syndrome. Gastroenterology. 1985 Mar;88(3):738–749. doi: 10.1016/0016-5085(85)90145-3. [DOI] [PubMed] [Google Scholar]
  15. Gannon J. A survey of Encephalitozoon cuniculi in laboratory animal colonies in the United Kingdom. Lab Anim. 1980 Apr;14(2):91–94. doi: 10.1258/002367780780942917. [DOI] [PubMed] [Google Scholar]
  16. Gannon J. The course of infection of Encephalitozoon cuniculi in immunodeficient and immunocompetent mice. Lab Anim. 1980 Jul;14(3):189–192. doi: 10.1258/002367780780937652. [DOI] [PubMed] [Google Scholar]
  17. Gannon J. The immunoperoxidase test diagnosis of Encephalitozoon cuniculi in rabbits. Lab Anim. 1978 Jul;12(3):125–127. doi: 10.1258/002367778780936322. [DOI] [PubMed] [Google Scholar]
  18. Gordon S. C., Reddy K. R., Gould E. E., McFadden R., O'Brien C., De Medina M., Jeffers L. J., Schiff E. R. The spectrum of liver disease in the acquired immunodeficiency syndrome. J Hepatol. 1986;2(3):475–484. doi: 10.1016/s0168-8278(86)80059-9. [DOI] [PubMed] [Google Scholar]
  19. Hollister W. S., Canning E. U. An enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to Encephalitozoon cuniculi and its use in determination of infections in man. Parasitology. 1987 Apr;94(Pt 2):209–219. doi: 10.1017/s0031182000053890. [DOI] [PubMed] [Google Scholar]
  20. Hunt R. D., King N. W., Foster H. L. Encephalitozoonosis: evidence for vertical transmission. J Infect Dis. 1972 Aug;126(2):212–214. doi: 10.1093/infdis/126.2.212. [DOI] [PubMed] [Google Scholar]
  21. Kemp R. L., Kluge J. P. Encephalitozoon sp. in the blue-masked lovebird, Agapornis personata (Reichenow): first confirmed report of microsporidan infection in birds. J Protozool. 1975 Nov;22(4):489–491. doi: 10.1111/j.1550-7408.1975.tb05214.x. [DOI] [PubMed] [Google Scholar]
  22. Ledford D. K., Overman M. D., Gonzalvo A., Cali A., Mester S. W., Lockey R. F. Microsporidiosis myositis in a patient with the acquired immunodeficiency syndrome. Ann Intern Med. 1985 May;102(5):628–630. doi: 10.7326/0003-4819-102-5-628. [DOI] [PubMed] [Google Scholar]
  23. Levine N. D., Corliss J. O., Cox F. E., Deroux G., Grain J., Honigberg B. M., Leedale G. F., Loeblich A. R., 3rd, Lom J., Lynn D. A newly revised classification of the protozoa. J Protozool. 1980 Feb;27(1):37–58. doi: 10.1111/j.1550-7408.1980.tb04228.x. [DOI] [PubMed] [Google Scholar]
  24. MATSUBAYASHI H., KOIKE T., MIKATA I., TAKEI H., HAGIWARA S. A case of Encephalitozoon-like body infection in man. AMA Arch Pathol. 1959 Feb;67(2):181–187. [PubMed] [Google Scholar]
  25. Macher A. M., Neafie R., Angritt P., Tuur S. M. Microsporidial myositis and the acquired immunodeficiency syndrome (AIDS): a four-year follow-up. Ann Intern Med. 1988 Aug 15;109(4):343–343. doi: 10.7326/0003-4819-109-4-343_1. [DOI] [PubMed] [Google Scholar]
  26. Marcus P. B., Van der Walt J. J., Burger P. J. Human tumor microsporidiosis. First reported case. Arch Pathol. 1973 May;95(5):341–343. [PubMed] [Google Scholar]
  27. Margileth A. M., Strano A. J., Chandra R., Neafie R., Blum M., McCully R. M. Disseminated nosematosis in an immunologically compromised infant. Arch Pathol. 1973 Mar;95(3):145–150. [PubMed] [Google Scholar]
  28. McCully R. M., Van Dellen A. F., Basson P. A., Lawrence J. Observations on the pathology of canine microsporidiosis. Onderstepoort J Vet Res. 1978 Jun;45(2):75–91. [PubMed] [Google Scholar]
  29. Modigliani R., Bories C., Le Charpentier Y., Salmeron M., Messing B., Galian A., Rambaud J. C., Lavergne A., Cochand-Priollet B., Desportes I. Diarrhoea and malabsorption in acquired immune deficiency syndrome: a study of four cases with special emphasis on opportunistic protozoan infestations. Gut. 1985 Feb;26(2):179–187. doi: 10.1136/gut.26.2.179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Mohn S. F. Encephalitozoonosis in the blue fox. Comparison between the india-ink immuno-reaction and the indirect fluorescent antibody test in detecting Encephalitozoon cuniculi antibodies. Acta Vet Scand. 1982;23(1):99–106. doi: 10.1186/BF03546826. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Mohn S. F., Nordstoga K., Møller O. M. Experimental encephalitozoonosis in the blue fox. Transplacental transmission of the parasite. Acta Vet Scand. 1982;23(2):211–220. doi: 10.1186/BF03546807. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Montrey R. D., Shadduck J. A., Pakes S. P. In-vitro study of host range of three isolates of Encephalitozoon (Nosema). J Infect Dis. 1973 Apr;127(4):450–454. doi: 10.1093/infdis/127.4.450. [DOI] [PubMed] [Google Scholar]
  33. Niederkorn J. Y., Brieland J. K., Mayhew E. Enhanced natural killer cell activity in experimental murine encephalitozoonosis. Infect Immun. 1983 Jul;41(1):302–307. doi: 10.1128/iai.41.1.302-307.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Niederkorn J. Y., Shadduck J. A., Weidner E. Antigenic cross-reactivity among different microsporidan spores as determined by immunofluorescence. J Parasitol. 1980 Aug;66(4):675–677. [PubMed] [Google Scholar]
  35. Pakes S. P., Shadduck J. A., Cali A. Fine structure of Encephalitozoon cuniculi from rabbits, mice and hamsters. J Protozool. 1975 Nov;22(4):481–488. doi: 10.1111/j.1550-7408.1975.tb05213.x. [DOI] [PubMed] [Google Scholar]
  36. Pakes S. P., Shadduck J. A., Feldman D. B., Moore J. A. Comparison of tests for the diagnosis of spontaneous encephalitozoonosis in rabbits. Lab Anim Sci. 1984 Aug;34(4):356–359. [PubMed] [Google Scholar]
  37. Petri M. The occurrence of Nosema cuniculi (Encephalitozoon cuniculi) in the cells of transplantable, malignant ascites tumours and its effect upon tumour and host. Acta Pathol Microbiol Scand. 1966;66(1):13–30. doi: 10.1111/apm.1966.66.1.13. [DOI] [PubMed] [Google Scholar]
  38. Pinnolis M., Egbert P. R., Font R. L., Winter F. C. Nosematosis of the cornea. Case report, including electron microscopic studies. Arch Ophthalmol. 1981 Jun;99(6):1044–1047. doi: 10.1001/archopht.1981.03930011044012. [DOI] [PubMed] [Google Scholar]
  39. Rijpstra A. C., Canning E. U., Van Ketel R. J., Eeftinck Schattenkerk J. K., Laarman J. J. Use of light microscopy to diagnose small-intestinal microsporidiosis in patients with AIDS. J Infect Dis. 1988 Apr;157(4):827–831. doi: 10.1093/infdis/157.4.827. [DOI] [PubMed] [Google Scholar]
  40. Schmidt E. C., Shadduck J. A. Mechanisms of resistance to the intracellular protozoan Encephalitozoon cuniculi in mice. J Immunol. 1984 Nov;133(5):2712–2719. [PubMed] [Google Scholar]
  41. Schmidt E. C., Shadduck J. A. Murine encephalitozoonosis model for studying the host-parasite relationship of a chronic infection. Infect Immun. 1983 Jun;40(3):936–942. doi: 10.1128/iai.40.3.936-942.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. Seibold H. R., Fussell E. N. Intestinal microsporidiosis in Callicebus moloch. Lab Anim Sci. 1973 Feb;23(1):115–118. [PubMed] [Google Scholar]
  43. Shadduck J. A., Bendele R., Robinson G. T. Isolation of the causative organism of canine encephalitozoonosis. Vet Pathol. 1978 Jul;15(4):449–460. doi: 10.1177/030098587801500402. [DOI] [PubMed] [Google Scholar]
  44. Shadduck J. A. Effect of fumagillin on in vitro multiplication of Encephalitozoon cuniculi. J Protozool. 1980 May;27(2):202–208. doi: 10.1111/j.1550-7408.1980.tb04681.x. [DOI] [PubMed] [Google Scholar]
  45. Shadduck J. A. Nosema cuiculi: in vitro isolation. Science. 1969 Oct 24;166(3904):516–517. doi: 10.1126/science.166.3904.516. [DOI] [PubMed] [Google Scholar]
  46. Shadduck J. A., Pakes S. P. Encephalitozoonosis (nosematosis) and toxoplasmosis. Am J Pathol. 1971 Sep;64(3):657–672. [PMC free article] [PubMed] [Google Scholar]
  47. Shadduck J. A., Polley M. B. Some factors influencing the in vitro infectivity and replication of Encephalitozoon cuniculi. J Protozool. 1978 Nov;25(4):491–496. doi: 10.1111/j.1550-7408.1978.tb04174.x. [DOI] [PubMed] [Google Scholar]
  48. Singh M., Kane G. J., Mackinlay L., Quaki I., Yap E. H., Ho B. C., Ho L. C., Lim K. C. Detection of antibodies to Nosema cuniculi (Protozoa : Microscoporidia) in human and animal sera by the indirect fluorescent antibody technique. Southeast Asian J Trop Med Public Health. 1982 Mar;13(1):110–113. [PubMed] [Google Scholar]
  49. Terada S., Reddy K. R., Jeffers L. J., Cali A., Schiff E. R. Microsporidan hepatitis in the acquired immunodeficiency syndrome. Ann Intern Med. 1987 Jul;107(1):61–62. doi: 10.7326/0003-4819-107-1-61. [DOI] [PubMed] [Google Scholar]
  50. Undeen A. H., Alger N. E. Nosema algerae: infection of the white mouse by a mosquito parasite. Exp Parasitol. 1976 Aug;40(1):86–88. doi: 10.1016/0014-4894(76)90068-0. [DOI] [PubMed] [Google Scholar]
  51. Undeen A. H. Growth of Nosema algerae in pig kidney cell cultures. J Protozool. 1975 Feb;22(1):107–110. doi: 10.1111/j.1550-7408.1975.tb00951.x. [DOI] [PubMed] [Google Scholar]
  52. Vossbrinck C. R., Maddox J. V., Friedman S., Debrunner-Vossbrinck B. A., Woese C. R. Ribosomal RNA sequence suggests microsporidia are extremely ancient eukaryotes. 1987 Mar 26-Apr 1Nature. 326(6111):411–414. doi: 10.1038/326411a0. [DOI] [PubMed] [Google Scholar]
  53. Vossbrinck C. R., Woese C. R. Eukaryotic ribosomes that lack a 5.8S RNA. Nature. 1986 Mar 20;320(6059):287–288. doi: 10.1038/320287a0. [DOI] [PubMed] [Google Scholar]
  54. Waller T. Sensitivity of Encephalitozoon cuniculi to various temperatures, disinfectants and drugs. Lab Anim. 1979 Jul;13(3):227–230. doi: 10.1258/002367779780937753. [DOI] [PubMed] [Google Scholar]
  55. Zeman D. H., Baskin G. B. Encephalitozoonosis in squirrel monkeys (Saimiri sciureus). Vet Pathol. 1985 Jan;22(1):24–31. doi: 10.1177/030098588502200104. [DOI] [PubMed] [Google Scholar]

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