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. 1994 Apr;14(4):2404–2410. doi: 10.1128/mcb.14.4.2404

Targeting frequency for deletion vectors in embryonic stem cells.

H Zhang 1, P Hasty 1, A Bradley 1
PMCID: PMC358607  PMID: 8139544

Abstract

We analyzed the gene targeting frequencies and recombination products generated by a series of replacement deletion vectors which target the hprt (hypoxanthine phosphoribosyltransferase) locus in mouse embryonic stem cells. We found that the targeting frequency of a 19.2-kb deletion was comparable to that of a 3-kb deletion or a conventional replacement event in which a 1.7-kb fragment was inserted into the locus. We also observed different integration patterns for these deletion vectors. A result of this finding is that a wide range of genomic deletions in embryonic stem cells is feasible.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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