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. 1990 Jul;10(7):3792–3796. doi: 10.1128/mcb.10.7.3792

Transcription analysis of class II human leukocyte antigen genes from normal and immunodeficient B lymphocytes, using polymerase chain reaction.

M Bull 1, A van Hoef 1, J Gorski 1
PMCID: PMC360836  PMID: 2113177

Abstract

The RNA transcript levels of all human leukocyte antigen class II loci were determined from class II congenital immunodeficient B cells by polymerase chain reaction amplification of cDNA. No mRNA was observed under conditions in which 0.01% normal levels could be visualized. Pre-mRNA could be amplified from normal B cells but not from immunodeficient B cells, indicating a transcription defect.

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