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. 1989 Feb;9(2):828–830. doi: 10.1128/mcb.9.2.828

Methylation of replicating and nonreplicating DNA in the ciliate Tetrahymena thermophila.

G S Harrison 1, K M Karrer 1
PMCID: PMC362662  PMID: 2710125

Abstract

Methylation of adenine in replicating and nonreplicating DNA of the ciliate Tetrahymena thermophila was examined. In growing cells, 87% of the methylation occurred on the newly replicated daughter strand, but methylation was also detectable on the parental strand. Methylation of nonreplicating DNA from starved cells was demonstrated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blackburn E. H., Pan W. C., Johnson C. C. Methylation of ribosomal RNA genes in the macronucleus of Tetrahymena thermophila. Nucleic Acids Res. 1983 Aug 11;11(15):5131–5145. doi: 10.1093/nar/11.15.5131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cummings D. J., Tait A., Goddard J. M. Methylated bases in DNA from Paramecium aurelia. Biochim Biophys Acta. 1974 Nov 20;374(1):1–11. doi: 10.1016/0005-2787(74)90194-4. [DOI] [PubMed] [Google Scholar]
  3. Gorovsky M. A., Hattman S., Pleger G. L. ( 6 N)methyl adenine in the nuclear DNA of a eucaryote, Tetrahymena pyriformis. J Cell Biol. 1973 Mar;56(3):697–701. doi: 10.1083/jcb.56.3.697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gorovsky M. A., Yao M. C., Keevert J. B., Pleger G. L. Isolation of micro- and macronuclei of Tetrahymena pyriformis. Methods Cell Biol. 1975;9(0):311–327. doi: 10.1016/s0091-679x(08)60080-1. [DOI] [PubMed] [Google Scholar]
  5. Gruenbaum Y., Szyf M., Cedar H., Razin A. Methylation of replicating and post-replicated mouse L-cell DNA. Proc Natl Acad Sci U S A. 1983 Aug;80(16):4919–4921. doi: 10.1073/pnas.80.16.4919. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Harrison G. S., Findly R. C., Karrer K. M. Site-specific methylation of adenine in the nuclear genome of a eucaryote, Tetrahymena thermophila. Mol Cell Biol. 1986 Jul;6(7):2364–2370. doi: 10.1128/mcb.6.7.2364. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Harrison G. S., Karrer K. M. DNA synthesis, methylation and degradation during conjugation in Tetrahymena thermophila. Nucleic Acids Res. 1985 Jan 11;13(1):73–87. doi: 10.1093/nar/13.1.73. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hattman S. DNA methylation of T-even bacteriophages and of their nonglucosylated mutants: its role in P1-directed restriction. Virology. 1970 Oct;42(2):359–367. doi: 10.1016/0042-6822(70)90279-5. [DOI] [PubMed] [Google Scholar]
  9. Hattman S., Kenny C., Berger L., Pratt K. Comparative study of DNA methylation in three unicellular eucaryotes. J Bacteriol. 1978 Sep;135(3):1156–1157. doi: 10.1128/jb.135.3.1156-1157.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hilliard J. K., Sneider T. W. Repair methylation of parental DNA in synchronized cultures of Novikoff hepatoma cells. Nucleic Acids Res. 1975 Jun;2(6):809–819. doi: 10.1093/nar/2.6.809. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kowalski J., Cheevers W. P. Synthesis of high molecular weight DNA strands during S phase. J Mol Biol. 1976 Jul 5;104(3):603–615. doi: 10.1016/0022-2836(76)90123-6. [DOI] [PubMed] [Google Scholar]
  12. Pratt K., Hattman S. Deoxyribonucleic acid methylation and chromatin organization in Tetrahymena thermophila. Mol Cell Biol. 1981 Jul;1(7):600–608. doi: 10.1128/mcb.1.7.600. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rae P. M., Spear B. B. Macronuclear DNA of the hypotrichous ciliate Oxytricha fallax. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4992–4996. doi: 10.1073/pnas.75.10.4992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Van Etten J. L., Schuster A. M., Girton L., Burbank D. E., Swinton D., Hattman S. DNA methylation of viruses infecting a eukaryotic Chlorella-like green alga. Nucleic Acids Res. 1985 May 24;13(10):3471–3478. doi: 10.1093/nar/13.10.3471. [DOI] [PMC free article] [PubMed] [Google Scholar]

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