Abstract
Growth of Rocky Mountain spotted fever (RMSF) rickettsiae in duck embryo cell (DEC) cultures and chicken embryo cell (CEC) cultures was evaluated. Experimental lots of duck embryo cell- and chicken embryo cell-grown Rocky Mountain spotted fever vaccines and a commercial lot of yolk sac-grown vaccine were compared for protective efficacy in rhesus monkeys. Incidence and magnitude of antibody response, febrile response, and rickettsemia, as well as incidence of fatalities, suggested that both cell culture-derived vaccines were more immunogenic than the yolk sac-grown vaccine.
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- DuPont H. L., Hornick R. B., Dawkins A. T., Heiner G. G., Fabrikant I. B., Wisseman C. L., Jr, Woodward T. E. Rocky Mountain spotted fever: a comparative study of the active immunity induced by inactivated and viable pathogenic Rickettsia rickettsii. J Infect Dis. 1973 Sep;128(3):340–344. doi: 10.1093/infdis/128.3.340. [DOI] [PubMed] [Google Scholar]
- Fiset P., Ormsbee R. A., Silberman R., Peacock M., Spielman S. H. A microagglutination technique for detection and measurement of rickettsial antibodies. Acta Virol. 1969 Jan;13(1):60–66. [PubMed] [Google Scholar]
- Kenyon R. H., Acree W. M., Wright G. G., Melchior F. W., Jr Preparation of vaccines for Rocky Mountain spotted fever from rickettsiae propagated in cell culture. J Infect Dis. 1972 Feb;125(2):146–152. doi: 10.1093/infdis/125.2.146. [DOI] [PubMed] [Google Scholar]
- Kenyon R. H., Pedersen C. E., Jr Preparation of Rocky Mountain spotted fever vaccine suitable for human immunization. J Clin Microbiol. 1975 Jun;1(6):500–503. doi: 10.1128/jcm.1.6.500-503.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Silberman R., Fiset P. Method for counting Rickettsiae and Chlamydiae in purified suspensions. J Bacteriol. 1968 Jan;95(1):259–261. doi: 10.1128/jb.95.1.259-261.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weinberg E. H., Stakebake J. R., Gerone P. J. Plaque assay for Rickettsia rickettsii. J Bacteriol. 1969 May;98(2):398–402. doi: 10.1128/jb.98.2.398-402.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wike D. A., Burgdorfer W. Plaque formation in tissue cultures by Rickettsia rickettsi isolated directly from whole blood and tick hemolymph. Infect Immun. 1972 Nov;6(5):736–738. doi: 10.1128/iai.6.5.736-738.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]