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. 1986 Apr;23(4):783–785. doi: 10.1128/jcm.23.4.783-785.1986

Identification of mycoplasma colonies by immunobinding.

H Kotani, G J McGarrity
PMCID: PMC362837  PMID: 3084556

Abstract

An immunobinding assay was developed to identify mycoplasma colonies on agar in pure and mixed cultures. Mycoplasma colonies on agar were transferred to nitrocellulose. The nitrocellulose was treated with specific rabbit antisera against mycoplasmas, peroxidase-conjugated goat anti-rabbit immunoglobulin G, 4-chloro-1-naphthol, and H2O2. Purple developed in the presence of specific reactions. The procedure was superior to epifluorescence.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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