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. 1989 May;9(5):1965–1968. doi: 10.1128/mcb.9.5.1965

Hygromycin resistance as a selectable marker in Dictyostelium discoideum.

T T Egelhoff 1, S S Brown 1, D J Manstein 1, J A Spudich 1
PMCID: PMC362988  PMID: 2546056

Abstract

We have constructed an expression cartridge which has the bacterial hygromycin resistance gene (hph) fused to the Dictyostelium discoideum actin 15 promoter, with a segment of 3'-flanking DNA from the actin 15 locus placed downstream of the hph gene to serve as a transcription terminator. The plasmid pDE109, which contained this cartridge and a Dictyostelium origin of replication, transformed D. discoideum with high efficiency under hygromycin selection. The availability of this selectable marker circumvents the previous limitation of having G418 resistance as the only selectable marker for this organism; secondary transformation can now be used to introduce DNA into previously transformed cell lines.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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