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. 1988 Jan;8(1):186–195. doi: 10.1128/mcb.8.1.186

Structure and expression of the human L-myc gene reveal a complex pattern of alternative mRNA processing.

F Kaye 1, J Battey 1, M Nau 1, B Brooks 1, E Seifter 1, J De Greve 1, M Birrer 1, E Sausville 1, J Minna 1
PMCID: PMC363100  PMID: 2827002

Abstract

We analyzed in detail the structure of the L-myc gene isolated from human placental DNA and characterized its expression in several small-cell lung cancer cell lines. The gene is composed of three exons and two introns spanning 6.6 kilobases in human DNA. Several distinct mRNA species are produced in all small-cell lung cancer cell lines that express L-myc. These transcripts are generated from a single gene by alternative splicing of introns 1 and 2 and by use of alternative polyadenylation signals. In some mRNAs there is a long open reading frame with a predicted translated protein of 364 residues. Amino acid sequence comparison with c-myc and N-myc demonstrated multiple discrete regions with extensive homology. In contrast, other mRNA transcripts, generated by alternative processing, could encode a truncated protein with a novel carboxy-terminal end.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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