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. 1988 Mar;8(3):1380–1384. doi: 10.1128/mcb.8.3.1380

Separation of simian virus 40 large-T-antigen-transforming and origin-binding functions from the ability to block differentiation.

V Cherington 1, M Brown 1, E Paucha 1, J St Louis 1, B M Spiegelman 1, T M Roberts 1
PMCID: PMC363287  PMID: 2835674

Abstract

Wild-type simian virus 40 large T antigen is very effective at blocking adipocyte differentiation in 3T3-F442A cells as assayed by triglyceride accumulation, induction of glycerophosphate dehydrogenase activity, and expression of mRNAs for glycerophosphate dehydrogenase, the adipocyte serine protease adipsin, and the putative lipid-binding protein adipocyte P2. Point mutants defective for either origin-specific DNA binding or transformation blocked differentiation as completely as wild type.

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Selected References

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