Skip to main content
PMC home page
Molecular and Cellular Biology logoLink to Molecular and Cellular Biology
. 1988 Dec;8(12):5545–5548. doi: 10.1128/mcb.8.12.5545

Effect of c-myc gene expression on early inducible reactions required for erythroid differentiation in vitro.

T Kaneko-Ishino 1, T U Kume 1, H Sasaki 1, M Obinata 1, M Oishi 1
PMCID: PMC365659  PMID: 3244364

Abstract

By employing cell fusion between two genetically marked mouse erythroleukemia (MEL) cells in which an artificially introduced c-myc gene had been placed under the control of human metallothionein promoter, we investigated the mechanism of the suppressive action of c-myc gene expression in erythroid differentiation. The results indicated that the expression of the c-myc gene blocked the induction of dimethyl sulfoxide-inducible activity, one of the two early activities required for triggering the differentiation.

Full text

PDF
5545

Images in this article

5546Image
on p.5546

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Coppola J. A., Cole M. D. Constitutive c-myc oncogene expression blocks mouse erythroleukaemia cell differentiation but not commitment. Nature. 1986 Apr 24;320(6064):760–763. doi: 10.1038/320760a0. [DOI] [PubMed] [Google Scholar]
  2. Dmitrovsky E., Kuehl W. M., Hollis G. F., Kirsch I. R., Bender T. P., Segal S. Expression of a transfected human c-myc oncogene inhibits differentiation of a mouse erythroleukaemia cell line. Nature. 1986 Aug 21;322(6081):748–750. doi: 10.1038/322748a0. [DOI] [PubMed] [Google Scholar]
  3. Friend C., Patuleia M. C., De Harven E. Erythrocytic maturation in vitro of murine (Friend) virus-induced leukemic cells. Natl Cancer Inst Monogr. 1966 Sep;22:505–522. [PubMed] [Google Scholar]
  4. Friend C., Scher W., Holland J. G., Sato T. Hemoglobin synthesis in murine virus-induced leukemic cells in vitro: stimulation of erythroid differentiation by dimethyl sulfoxide. Proc Natl Acad Sci U S A. 1971 Feb;68(2):378–382. doi: 10.1073/pnas.68.2.378. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kaneko T., Nomura S., Oishi M. Early events leading to erythroid differentiation in mouse Friend cells revealed by cell fusion experiments. Cancer Res. 1984 May;44(5):1756–1760. [PubMed] [Google Scholar]
  6. Kume T. U., Takada S., Obinata M. Probability that the commitment of murine erythroleukemia cell differentiation is determined by the c-myc level. J Mol Biol. 1988 Aug 20;202(4):779–786. doi: 10.1016/0022-2836(88)90558-x. [DOI] [PubMed] [Google Scholar]
  7. Lachman H. M., Cheng G. H., Skoultchi A. I. Transfection of mouse erythroleukemia cells with myc sequences changes the rate of induced commitment to differentiate. Proc Natl Acad Sci U S A. 1986 Sep;83(17):6480–6484. doi: 10.1073/pnas.83.17.6480. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lachman H. M., Hatton K. S., Skoultchi A. I., Schildkraut C. L. c-myc mRNA levels in the cell cycle change in mouse erythroleukemia cells following inducer treatment. Proc Natl Acad Sci U S A. 1985 Aug;82(16):5323–5327. doi: 10.1073/pnas.82.16.5323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lachman H. M., Skoultchi A. I. Expression of c-myc changes during differentiation of mouse erythroleukaemia cells. Nature. 1984 Aug 16;310(5978):592–594. doi: 10.1038/310592a0. [DOI] [PubMed] [Google Scholar]
  10. Nomura S., Oishi M. Indirect induction of erythroid differentiation in mouse Friend cells: evidence for two intracellular reactions involved in the differentiation. Proc Natl Acad Sci U S A. 1983 Jan;80(1):210–214. doi: 10.1073/pnas.80.1.210. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nomura S., Yamagoe S., Kamiya T., Oishi M. An intracellular factor that induces erythroid differentiation in mouse erythroleukemia (Friend) cells. Cell. 1986 Feb 28;44(4):663–669. doi: 10.1016/0092-8674(86)90275-8. [DOI] [PubMed] [Google Scholar]
  12. Prochownik E. V., Kukowska J. Deregulated expression of c-myc by murine erythroleukaemia cells prevents differentiation. 1986 Aug 28-Sep 3Nature. 322(6082):848–850. doi: 10.1038/322848a0. [DOI] [PubMed] [Google Scholar]
  13. Ramsay R. G., Ikeda K., Rifkind R. A., Marks P. A. Changes in gene expression associated with induced differentiation of erythroleukemia: protooncogenes, globin genes, and cell division. Proc Natl Acad Sci U S A. 1986 Sep;83(18):6849–6853. doi: 10.1073/pnas.83.18.6849. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Reuben R. C., Wife R. L., Breslow R., Rifkind R. A., Marks P. A. A new group of potent inducers of differentiation in murine erythroleukemia cells. Proc Natl Acad Sci U S A. 1976 Mar;73(3):862–866. doi: 10.1073/pnas.73.3.862. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Scher W., Friend C. Breakage of DNA and alterations in folded genomes by inducers of differentiation in Friend erythroleukemic cells. Cancer Res. 1978 Mar;38(3):841–849. [PubMed] [Google Scholar]
  16. Terada M., Nudel U., Fibach E., Rifkind R. A., Marks P. A. Changes in DNA associated with induction of erythroid differentiation by dimethyl sulfoxide in murine erythroleukemia cells. Cancer Res. 1978 Mar;38(3):835–840. [PubMed] [Google Scholar]
  17. Watanabe T., Nomura S., Oishi M. Induction of erythroid differentiation by cytoplast fusion in mouse erythroleukemia (Friend) cells. Exp Cell Res. 1985 Jul;159(1):224–234. doi: 10.1016/s0014-4827(85)80051-3. [DOI] [PubMed] [Google Scholar]
  18. Watanabe T., Oishi M. Dimethyl sulfoxide-inducible cytoplasmic factor involved in erythroid differentiation in mouse erythroleukemia (Friend) cells. Proc Natl Acad Sci U S A. 1987 Sep;84(18):6481–6485. doi: 10.1073/pnas.84.18.6481. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Molecular and Cellular Biology are provided here courtesy of Taylor & Francis

RESOURCES