Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1972 Jan;49(1):82–86. doi: 10.1104/pp.49.1.82

Sugar Transport in Immature Internodal Tissue of Sugarcane

I. Mechanism and Kinetics of Accumulation 1

John E Bowen a
PMCID: PMC365904  PMID: 16657901

Abstract

Transmembrane sugar transport into immature internodal parenchyma tissue of sugarcane (Saccharum officinarum L.) is a metabolically regulated process as evidenced by its sensitivity to pH, temperature, anaerobiosis, and metabolic inhibitors. All sugars studied—glucose, fructose, galactose, sorbose, glucose 6-phosphate, 3-O-methylglucose, and 2-deoxy-d-glucose—were apparently transported via the same carrier sites since they competed with each other for uptake. External concentrations of these sugars at one-half Vmax were in the range of 3.9 to 8.4 nm. Preliminary data indicated that phosphorylation may be closely associated with glucose transport. The dominant intracellular sugar after 4-hours incubation was sucrose when glucose, glucose-6-P, or fructose was the exogenously supplied sugar; but when galactose was supplied, only 28% of intracellular radioactivity was in sucrose. Sorbose, 3-O-methylglucose, and 2-deoxy-d-glucose were not metabolized. Thus, by using these analogs, transport could be studied independently of subsequent metabolism, effectively eliminating a complicating factor in previous studies.

Full text

PDF
84

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Glasziou K. T. Accumulation & transformation of sugars in stalks of sugar cane. Origin of glucose & fructose in the inner space. Plant Physiol. 1961 Mar;36(2):175–179. doi: 10.1104/pp.36.2.175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Glasziou K. T. Accumulation and Transformation of Sugars in Sugar Cane Stalks. Plant Physiol. 1960 Nov;35(6):895–901. doi: 10.1104/pp.35.6.895. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. HOFSTEE B. H. J. On the evaluation of the constants Vm and KM in enzyme reactions. Science. 1952 Sep 26;116(3013):329–331. doi: 10.1126/science.116.3013.329. [DOI] [PubMed] [Google Scholar]
  4. Hatch M. D., Sacher J. A., Glasziou K. T. Sugar Accumulation Cycle in Sugar Cane. I. Studies on Enzymes of the Cycle. Plant Physiol. 1963 May;38(3):338–343. doi: 10.1104/pp.38.3.338. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hatch M. D. Sugar accumulation by sugar-cane storage tissue: the role of sucrose phosphate. Biochem J. 1964 Dec;93(3):521–526. doi: 10.1042/bj0930521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Pardee A. B., Watanabe K. Location of sulfate-binding protein in Salmonella typhimurium. J Bacteriol. 1968 Oct;96(4):1049–1054. doi: 10.1128/jb.96.4.1049-1054.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Reinhold L., Eshhar Z. Transport of 3-o-Methylglucose Into and Out of Storage Cells of Daucus carota. Plant Physiol. 1968 Jul;43(7):1023–1030. doi: 10.1104/pp.43.7.1023. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES