Skip to main content
Plant Physiology logoLink to Plant Physiology
. 1972 Mar;49(3):398–403. doi: 10.1104/pp.49.3.398

The Levels of Soluble Nucleotides in Wheat Aleurone Tissue 1

G G Collins a, C F Jenner a, L G Paleg a
PMCID: PMC365973  PMID: 16657969

Abstract

The content of soluble nucleotides in aleurone layers isolated from mature wheat (Triticum aestivum var. Olympic) grain was investigated. The most abundant nucleotides were adenosine triphosphate, uridine triphosphate, and uridine diphosphoglucose. Smaller amounts of guanosine triphosphate, cytidine triphosphate, adenosine diphosphate, and nicotinamide adenine dinucleotide were also identified. The levels of some of these nucleotides were increased after incubation of the tissue under certain conditions.

Nucleotide levels were measured at intervals during incubation of aleurone layers in water. The changes observed are discussed in relation to a response by the tissue to wounding.

Full text

PDF
399

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMOS H., KORN M. 5-Methyl cytosine in the RNA of Escherichia coli. Biochim Biophys Acta. 1958 Aug;29(2):444–445. doi: 10.1016/0006-3002(58)90214-2. [DOI] [PubMed] [Google Scholar]
  2. Bieleski R. L. Effect of phosphorus deficiency on levels of phosphorus compounds in spirodela. Plant Physiol. 1968 Aug;43(8):1309–1316. doi: 10.1104/pp.43.8.1309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bieleski R. L. Phosphorus compounds in translocating Phloem. Plant Physiol. 1969 Apr;44(4):497–502. doi: 10.1104/pp.44.4.497. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CAPUTTO R., LELOIR L. F., CARDINI C. E., PALADINI A. C. Isolation of the coenzyme of the galactose phosphate-glucose phosphate transformation. J Biol Chem. 1950 May;184(1):333–350. [PubMed] [Google Scholar]
  5. CARMINATTI H., PASSERON S., DANKERT M., RECONDO E. SEPARATION OF SUGAR NUCLEOTIDES, PHOSPHORIC ESTERS AND FREE SUGARS BY PAPER CHROMATOGRAPHY WITH SOLVENTS CONTAINING BORATES OF ORGANIC BASES. J Chromatogr. 1965 May;18:342–348. doi: 10.1016/s0021-9673(01)80372-1. [DOI] [PubMed] [Google Scholar]
  6. Chrispeels M. J., Varner J. E. Gibberellic Acid-enhanced synthesis and release of alpha-amylase and ribonuclease by isolated barley and aleurone layers. Plant Physiol. 1967 Mar;42(3):398–406. doi: 10.1104/pp.42.3.398. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chrispeels M. J., Varner J. E. Hormonal control of enzyme synthesis: on the mode of action of gibberellic Acid and abscisin in aleurone layers of barley. Plant Physiol. 1967 Jul;42(7):1008–1016. doi: 10.1104/pp.42.7.1008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cole C. V., Ross C. Extraction, separation, and quantitative estimation of soluble nucleotides and sugar phosphates in plant tissues. Anal Biochem. 1966 Dec;17(3):526–539. doi: 10.1016/0003-2697(66)90188-6. [DOI] [PubMed] [Google Scholar]
  9. Collins G. G., Jenner C. F., Paleg L. G. The metabolism of soluble nucleotides in wheat aleurone layers treated with gibberellic Acid. Plant Physiol. 1972 Mar;49(3):404–410. doi: 10.1104/pp.49.3.404. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Isherwood F. A., Barrett F. C. Analysis of phosphate esters in plant material. Extraction and purification. Biochem J. 1967 Sep;104(3):922–933. doi: 10.1042/bj1040922. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jenner C. F. The composition of soluble nucleotides in the developing wheat grain. Plant Physiol. 1968 Jan;43(1):41–49. doi: 10.1104/pp.43.1.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lichtman M. A., Miller D. R. Erythrocyte glycolysis, 2,3-diphosphoglycerate and adenosine triphosphate concentration in uremic subjects: relationship to extracellular phosphate concentration. J Lab Clin Med. 1970 Aug;76(2):267–279. [PubMed] [Google Scholar]
  13. PORATH J. Use of triethlammonium buffers in ion-exchange chromatography and electrophoresis. Nature. 1955 Mar 12;175(4454):478–478. doi: 10.1038/175478a0. [DOI] [PubMed] [Google Scholar]
  14. Paleg L. G., Coombe B. G., Buttrose M. S. Physiological effects of gibberellic acid. V. Endosperm responses of barley, wheat, & oats. Plant Physiol. 1962 Nov;37(6):798–803. doi: 10.1104/pp.37.6.798. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pollard C. J., Singh B. N. Early effects of gibberellic acid on barley aleurone layers. Biochem Biophys Res Commun. 1968 Oct 24;33(2):321–326. doi: 10.1016/0006-291x(68)90787-0. [DOI] [PubMed] [Google Scholar]
  16. Varner J. E., Chandra G. R. HORMONAL CONTROL OF ENZYME SYNTHESIS IN BARLEY ENDOSPERM. Proc Natl Acad Sci U S A. 1964 Jul;52(1):100–106. doi: 10.1073/pnas.52.1.100. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. WYATT G. R. The purine and pyrimidine composition of deoxypentose nucleic acids. Biochem J. 1951 May;48(5):584–590. doi: 10.1042/bj0480584. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES