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. 1972 May;49(5):783–788. doi: 10.1104/pp.49.5.783

Characterization of Short Time Labeled Adenosine Monophosphate-rich Ribonucleic Acids of Soybean 1

Joe L Key a, C J Leaver a,2, J R Cowles a,3, J M Anderson a
PMCID: PMC366052  PMID: 16658048

Abstract

The total population of newly synthesized 32P-AMP-rich RNA has been separated into two major types based on repeated fractionation on methylated albumin-kieselguhr columns. The purified D-RNA which elutes, under our experimental conditions, primarily in the salt gradient has a GMP/AMP ratio of about 0.8 and an AMP + UMP content of about 56 mole per cent. The purified TB-RNA which preferentially remains bound to the column in the salt gradient has a GMP/AMP ratio of about 0.4 to 0.45 and an AMP + UMP content of about 65 mole per cent. In addition to being distinguished by their fractionation on the methylated albumin-kieselguhr column and base composition analysis, purified D-RNA and TB-RNA have different size distributions on sucrose gradient and acrylamide gel fractionation, are differentially associated with polyribosomes and have different stabilities in the tissue.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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