Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1972 Jun;49(6):1000–1006. doi: 10.1104/pp.49.6.1000

A Correlation between a Ribonucleic Acid Fraction Selectively Labeled in the Presence of Gibberellic Acid and Amylase Synthesis in Barley Aleurone Layers

J A Zwar a, John V Jacobsen a
PMCID: PMC366097  PMID: 16658066

Abstract

The effects of gibberellic acid on the incorporation of radio-active uridine and adenosine into RNA of barley aleurone layers were investigated using a double labeling method combined with acrylamide gel electrophoresis. After 16 hours of incubation, gibberellic acid stimulated the incorporation of label into all species of RNA, but the effects were very small (0-10%) for ribosomal and transfer RNA and comparatively large (up to 300%) for RNA sedimenting between 5S and 14S. This result was obtained for both isolated aleurone layers and for layers still attached to the endosperm. A similar but less marked pattern occurred in layers incubated for 8 hours, but the effect was not observed after 4 hours. The gibberellic acid-enhanced RNA labeling was not due to micro-organisms. The following evidence was obtained for an association between the gibberellic acid-enhanced RNA synthesis and α-amylase synthesis: (a) synthesis of α-amylase took place in parallel with incorporation of label into gibberellic acid-RNA; (b) actinomycin D inhibited amylase synthesis and gibberellic acid-RNA by similar percentages; (c) 5-fluorouracil halved incorporation of label into ribosomal RNA but had no effect on amylase synthesis and gibberellic acid-RNA; and (d) abscisic acid had little effect on synthesis of RNA in the absence of gibberellic acid, but when it was included with gibberellic acid the synthesis of both enzyme and gibberellic acid-RNA was eliminated. We conclude that large changes in the synthesis of the major RNA species are not necessary for α-amylase synthesis to occur but that α-amylase synthesis does not occur without the production of gibberrellic acid-RNA. Gibberellic acid-RNA is probably less than 1% of the total tissue RNA, is polydisperse on acrylamide gels, and could be messenger species for α-amylase and other hydrolytic enzymes whose synthesis is under gibberellic acid control.

Full text

PDF
1002

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chandra G. R., Varner J. E. Gibberellic acid-controlled metabolism of RNA in aleurone cells of barley. Biochim Biophys Acta. 1965 Dec 9;108(4):583–592. doi: 10.1016/0005-2787(65)90055-9. [DOI] [PubMed] [Google Scholar]
  2. Click R. E., Hackett D. P. The isolation of ribonucleic acid from plant, bacterial or animal cells. Biochim Biophys Acta. 1966 Oct 24;129(1):74–84. doi: 10.1016/0005-2787(66)90010-4. [DOI] [PubMed] [Google Scholar]
  3. Dingman C. W., Peacock A. C. Analytical studies on nuclear ribonucleic acid using polyacrylamide gel electrophoresis. Biochemistry. 1968 Feb;7(2):659–668. doi: 10.1021/bi00842a022. [DOI] [PubMed] [Google Scholar]
  4. Filner P., Varner J. E. A test for de novo synthesis of enzymes: density labeling with H2O18 of barley alpha-amylase induced by gibberellic acid. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1520–1526. doi: 10.1073/pnas.58.4.1520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ingle J., Burns R. G. The loss of ribosomal ribonucleic acid during the preparationof nucleic acid from certain plant tissues by the detergent-phenol method. Biochem J. 1968 Dec;110(3):605–606. doi: 10.1042/bj1100605. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jacobsen J. V., Varner J. E. Gibberellic Acid-induced synthesis of protease by isolated aleurone layers of barley. Plant Physiol. 1967 Nov;42(11):1596–1600. doi: 10.1104/pp.42.11.1596. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Paleg L. G. Physiological Effects of Gibberellic Acid: I. On Carbohydrate Metabolism and Amylase Activity of Barley Endosperm. Plant Physiol. 1960 May;35(3):293–299. doi: 10.1104/pp.35.3.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Patterson B. D., Trewavas A. J. Changes in the pattern of protein synthesis induced by 3-indolylacetic Acid. Plant Physiol. 1967 Aug;42(8):1081–1086. doi: 10.1104/pp.42.8.1081. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. RALPH R. K., BELLAMY A. R. ISOLATION AND PURIFICATION OF UNDEGRADED RIBONUCLEIC ACIDS. Biochim Biophys Acta. 1964 May 18;87:9–16. doi: 10.1016/0926-6550(64)90041-6. [DOI] [PubMed] [Google Scholar]
  10. Solymosy F., Fedorcsák I., Gulyás A., Farkas G. L., Ehrenberg L. A new method based on the use of diethyl pyrocarbonate as a nuclease inhibitor for the extraction of undegraded nucleic acid from plant tissues. Eur J Biochem. 1968 Sep 24;5(4):520–527. doi: 10.1111/j.1432-1033.1968.tb00401.x. [DOI] [PubMed] [Google Scholar]
  11. Varner J. E. Gibberellic Acid Controlled Synthesis of alpha-Amylase in Barley Endosperm. Plant Physiol. 1964 May;39(3):413–415. doi: 10.1104/pp.39.3.413. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES