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. 1972 Nov;50(5):616–621. doi: 10.1104/pp.50.5.616

N6-(Δ2-Isopentenyl)adenosine: Its Occurrence as a Free Nucleoside in an Autonomous Strain of Tobacco Tissue 1,2

W H Dyson a, R H Hall a
PMCID: PMC366201  PMID: 16658228

Abstract

Cytokinins from both the free nucleoside pool and the transfer RNA have been isolated and identified in a habituated strain of tobacco pith callus (Nicotiana tabacum [L] var. Wisconsin 38). The transfer RNA of this strain contains both N6-(Δ2-isopentenyl) adenosine and N6-(4-hydroxy-3-methylbut-2-cis-enyl) adenosine. The trans-hydroxylated derivative is absent from the transfer RNA of this dark-grown tissue. N6-(Δ2-Isopentenyl)-adenosine was identified as a component of the free nucleoside pool in concentrations of about 10 micrograms per kilogram of tissue.

The cytokinin-requiring tissue grown in the presence of N6-furfurylaminopurine contains less than 85 nanograms per kilogram of N6-(Δ2-isopentenyl) adenosine. This is the detectable limit under the conditions of the experiment.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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