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. 1973 Feb;51(2):409–411. doi: 10.1104/pp.51.2.409

Possible Errors in Quantitative Determination of Phenylalanine Ammonia-Lyase Activity by Spectrophotometric Methods 1

A Erez a
PMCID: PMC366277  PMID: 16658342

Abstract

A possible error in spectrophotometric determination of cinnamate, the product of phenylalanine ammonia-lyase activity, using nonpurified protein extracts has been shown.

Under optimal conditions for phenylalanine ammonia-lyase activity, with borate buffer and in the presence of α-keto acids, phenylpyruvate is produced and its enol tautomer-borate complex formed, strongly absorbs at 290 nanometers.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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