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. 1973 Apr;51(4):651–659. doi: 10.1104/pp.51.4.651

Chloroplast Maintenance and Partial Differentiation in Vitro1

Constantin A Rebeiz a,2, Susan Larson a, T Elliot Weier a, Paul A Castelfranco a
PMCID: PMC366324  PMID: 16658388

Abstract

Tissue homogenates, etioplasts, and developing chloroplasts were prepared from cucumber (Cumucis sativus L.) cotyledons in tris-sucrose. They were incubated aerobically in the dark or in the light at pH 7.7 in the presence or absence of a cofactor mixture containing coenzyme A, glutathione, potassium phosphate, methyl alcohol, magnesium, nicotinamide adenine dinucleotide, and adenosine triphosphate. These cofactors were previously shown to be essential for protochlorophyll and chlorophyll biosynthesis. Ultrastructural changes were monitored by electron microscopy. The following observations were made. (a) Crude homogenates contained agents which degraded etioplasts and developing chloroplasts. (b) Added cofactors were essential for the maintenance of the membrane structure; they were also implicated in the transformation of the prolamellar body in the absence and presence of light. (c) Light pretreatment of the cotyledons improved the maintenance of the developing chloroplast membranes during subsequent in vitro incubation. (d) In the presence of the cofactors, grana formation appeared to take place in the absence of nuclear-cytoplasmic control.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. De Greef J., Butler W. L., Roth T. F. Greening of etiolated bean leaves in far red light. Plant Physiol. 1971 Apr;47(4):457–464. doi: 10.1104/pp.47.4.457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. French C. S., Brown J. S., Lawrence M. C. Four universal forms of chlorophyll a. Plant Physiol. 1972 Mar;49(3):421–429. doi: 10.1104/pp.49.3.421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hardy S. I., Castelfranco P. A., Rebeiz C. A. Effect of the hypocotyl hook on greening in etiolated cucumber cotyledons. Plant Physiol. 1970 Nov;46(5):705–707. doi: 10.1104/pp.46.5.705. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Rebeiz C. A., Castelfranco P. A. Chlorophyll biosynthesis in a cell-free system from higher plants. Plant Physiol. 1971 Jan;47(1):33–37. doi: 10.1104/pp.47.1.33. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Rebeiz C. A., Castelfranco P. A. Protochlorophyll biosynthesis in a cell-free system from higher plants. Plant Physiol. 1971 Jan;47(1):24–32. doi: 10.1104/pp.47.1.24. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ridley S. M., Leech R. M. Division of chloroplasts in an artificial environment. Nature. 1970 Aug 1;227(5257):463–465. doi: 10.1038/227463a0. [DOI] [PubMed] [Google Scholar]
  7. Spurr A. R. A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res. 1969 Jan;26(1):31–43. doi: 10.1016/s0022-5320(69)90033-1. [DOI] [PubMed] [Google Scholar]
  8. Thorne S. W., Boardman N. K. Formation of chlorophyll B, and the fluorescence properties and photochemical activities of isolated plastids from greening pea seedlings. Plant Physiol. 1971 Feb;47(2):252–261. doi: 10.1104/pp.47.2.252. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Wellburn F. A., Wellburn A. R. Chlorophyll synthesis by isolated intact etioplasts. Biochem Biophys Res Commun. 1971 Nov 5;45(3):747–750. doi: 10.1016/0006-291x(71)90480-3. [DOI] [PubMed] [Google Scholar]

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