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. 1973 Apr;51(4):677–684. doi: 10.1104/pp.51.4.677

The Genes for Cytoplasmic Ribosomal Ribonucleic Acid in Higher Plants

N Steele Scott a,1, J Ingle a
PMCID: PMC366328  PMID: 16658392

Abstract

The genes for cytoplasmic ribosomal RNA are partially resolved from the bulk of the DNA by CsCl equilibrium centrifugation. Although in some plants the buoyant density of the ribosomal RNA genes is as expected from the base composition of ribosomal RNA, others show a large discrepancy which cannot be due to the presence of low G-C spacer-DNA. The cross-hybridization observed with 1.3 and 0.7 × 106 molecular weight ribosomal RNAs and DNA, which varies greatly with different plant species, is not due to contamination of the ribosomal RNAs, and is specific for the ribosomal DNA of each species, probably largely restricted to those sequences coding for the two stable ribosomal RNAs. The double reciprocal plot may be used for the extrapolation of saturation values only with caution, because in these cases such plots are not linear over the whole of the hybridization reaction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Attardi G., Huang P. C., Kabat S. Recognition of ribosomal RNA sites in DNA. II. The HeLa cell system. Proc Natl Acad Sci U S A. 1965 Jul;54(1):185–192. doi: 10.1073/pnas.54.1.185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Avery R. J., Midgley J. E. A new approach to the analysis of hybridization of bacterial nucleic acids. Analysis of the ribosomal ribonucleic acids of Bacillus subtilis. Biochem J. 1969 Nov;115(3):383–394. doi: 10.1042/bj1150383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Avery R. J., Midgley J. E., Pigott G. H. An analysis of the ribosomal ribonucleic acids of Escherichia coli by hybridization techniques. Biochem J. 1969 Nov;115(3):395–403. doi: 10.1042/bj1150395. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Birnstiel M. L., Chipchase M., Speirs J. The ribosomal RNA cistrons. Prog Nucleic Acid Res Mol Biol. 1971;11:351–389. doi: 10.1016/s0079-6603(08)60332-3. [DOI] [PubMed] [Google Scholar]
  5. Birnstiel M. L., Sells B. H., Purdom I. F. Kinetic complexity of RNA molecules. J Mol Biol. 1972 Jan 14;63(1):21–39. doi: 10.1016/0022-2836(72)90519-0. [DOI] [PubMed] [Google Scholar]
  6. Birnstiel M. L., Wallace H., Sirlin J. L., Fischberg M. Localization of the ribosomal DNA complements in the nucleolar organizer region of Xenopus laevis. Natl Cancer Inst Monogr. 1966 Dec;23:431–447. [PubMed] [Google Scholar]
  7. Birnstiel M., Speirs J., Purdom I., Jones K., Loening U. E. Properties and composition of the isolated ribosomal DNA satellite of Xenopus laevis. Nature. 1968 Aug 3;219(5153):454–463. doi: 10.1038/219454a0. [DOI] [PubMed] [Google Scholar]
  8. Bishop J. O. Interpretation of DNA-RNA hybridization data. Nature. 1969 Nov 8;224(5219):600–603. doi: 10.1038/224600a0. [DOI] [PubMed] [Google Scholar]
  9. Bishop J. O. The effect of genetic complexity on the time-course of ribonucleic acid-deoxyribonucleic acid hybridization. Biochem J. 1969 Aug;113(5):805–811. doi: 10.1042/bj1130805. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Brady T., Clutter M. E. Cytolocalization of ribosomal cistrons in plant polytene chromosomes. J Cell Biol. 1972 Jun;53(3):827–832. doi: 10.1083/jcb.53.3.827. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Brown D. D., Weber C. S. Gene linkage by RNA-DNA hybridization. I. Unique DNA sequences homologous to 4 s RNA, 5 s RNA and ribosomal RNA. J Mol Biol. 1968 Jun 28;34(3):661–680. doi: 10.1016/0022-2836(68)90188-5. [DOI] [PubMed] [Google Scholar]
  12. Ingle J., Possingham J. V., Wells R., Leaver C. J., Loening U. E. The properties of chloroplast ribosomal-RNA. Symp Soc Exp Biol. 1970;24:303–325. [PubMed] [Google Scholar]
  13. Ingle J., Sinclair J. Ribosomal RNA genes and plant development. Nature. 1972 Jan 7;235(5332):30–32. doi: 10.1038/235030a0. [DOI] [PubMed] [Google Scholar]
  14. Jeanteur P., Attardi G. Relationship between HeLa cell ribosomal RNA and its precursors studied by high resolution RNA-DNA hybridization. J Mol Biol. 1969 Oct 28;45(2):305–324. doi: 10.1016/0022-2836(69)90107-7. [DOI] [PubMed] [Google Scholar]
  15. Kirk J. T. Effect of methylation of cytosine residues on the buoyant density of DNA in caesium chloride solution. J Mol Biol. 1967 Aug 28;28(1):171–172. doi: 10.1016/s0022-2836(67)80087-1. [DOI] [PubMed] [Google Scholar]
  16. Leaver C. J., Ingle J. The molecular integrity of chloroplast ribosomal ribonucleic acid. Biochem J. 1971 Jun;123(2):235–243. doi: 10.1042/bj1230235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Loening U. E. The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochem J. 1967 Jan;102(1):251–257. doi: 10.1042/bj1020251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Mangiarotti G., Apirion D., Schlessinger D., Silengo L. Biosynthetic precursors of 30S and 50S ribosomal particles in Escherichia coli. Biochemistry. 1968 Jan;7(1):456–472. doi: 10.1021/bi00841a058. [DOI] [PubMed] [Google Scholar]
  19. Moore R. L., McCarthy B. J. Related base sequences in the DNA of simple and complex organisms. 3. Variability in the base sequence of the reduplicated genes for ribosomal RNA in the rabbit. Biochem Genet. 1968 Jun;2(1):75–86. doi: 10.1007/BF01458452. [DOI] [PubMed] [Google Scholar]
  20. Quagliarotti G., Ritossa F. M. On the arrangement of genes for 28 s and 18 s ribosomal RNA's in Drosophila melanogaster. J Mol Biol. 1968 Aug 28;36(1):57–69. doi: 10.1016/0022-2836(68)90219-2. [DOI] [PubMed] [Google Scholar]
  21. Retèl J., Planta R. J. The investigation of the ribosomal RNA sites in yeast DNA by the hybridization technique. Biochim Biophys Acta. 1968 Dec 17;169(2):416–429. doi: 10.1016/0005-2787(68)90050-6. [DOI] [PubMed] [Google Scholar]
  22. SCHILDKRAUT C. L., MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J Mol Biol. 1962 Jun;4:430–443. doi: 10.1016/s0022-2836(62)80100-4. [DOI] [PubMed] [Google Scholar]
  23. STUDIER F. W. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA. J Mol Biol. 1965 Feb;11:373–390. doi: 10.1016/s0022-2836(65)80064-x. [DOI] [PubMed] [Google Scholar]
  24. Steele W. J. Localization of deoxyribonucleic acid complementary to ribosomal ribonucleic acid and preribosomal ribonucleic acid in the nucleolus of rat liver. J Biol Chem. 1968 Jun 25;243(12):3333–3341. [PubMed] [Google Scholar]
  25. Wallace H., Birnstiel M. L. Ribosomal cistrons and the nucleolar organizer. Biochim Biophys Acta. 1966 Feb 21;114(2):296–310. doi: 10.1016/0005-2787(66)90311-x. [DOI] [PubMed] [Google Scholar]
  26. Wells R., Ingle J. The constancy of the buoyant density of chloroplast and mitochondrial deoxyribonucleic acids in a range of higher plants. Plant Physiol. 1970 Jul;46(1):178–179. doi: 10.1104/pp.46.1.178. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. YANKOFSKY S. A., SPIEGELMAN S. Distinct cistrons for the two ribosomal RNA components. Proc Natl Acad Sci U S A. 1963 Apr;49:538–544. doi: 10.1073/pnas.49.4.538. [DOI] [PMC free article] [PubMed] [Google Scholar]

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