Skip to main content
Plant Physiology logoLink to Plant Physiology
. 1973 Jul;52(1):50–53. doi: 10.1104/pp.52.1.50

Enzymes of Phospholipid Metabolism in the Endoplasmic Reticulum of Castor Bean Endosperm 1

T S Moore a, J M Lord a,2, T Kagawa a,3, Harry Beevers a
PMCID: PMC366436  PMID: 16658497

Abstract

The intracellular location of several enzymes concerned with phospholipid metabolism was investigated by examining their distribution in organelles separated on sucrose gradients from total homogenates of castor bean (Ricinus communis var. Hale) endosperm. The enzymes phosphatidic acid phosphatase, CDP-diglyceride-inositol transferase, and phosphatidyletha-nolamine-l-serine phosphatidyl transferase were all primarily or exclusively confined to membranes of the endoplasmic reticulum. These results and those reported previously on lecithin synthesis establish a major role of the endoplasmic reticulum in phospholipid and membrane synthesis in plant tissues.

Full text

PDF
52

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DILS R. R., HUBSCHER G. The incorporation in vitro of [Me-14C]choline into the phospholipids of rat-liver mitochondria. Biochim Biophys Acta. 1959 Mar;32(1):293–294. doi: 10.1016/0006-3002(59)90596-7. [DOI] [PubMed] [Google Scholar]
  2. Dennis E. A., Kennedy E. P. Intracellular sites of lipid synthesis and the biogenesis of mitochondria. J Lipid Res. 1972 Mar;13(2):263–267. [PubMed] [Google Scholar]
  3. Donaldson R. P., Tolbert N. E., Schnarrenberger C. A comparison of microbody membranes with microsomes and mitochondria from plant and animal tissue. Arch Biochem Biophys. 1972 Sep;152(1):199–215. doi: 10.1016/0003-9861(72)90208-1. [DOI] [PubMed] [Google Scholar]
  4. Franke W. W., Kartenbeck J. Outer mitochondrial membrane continuous with endoplasmic reticulum. Protoplasma. 1971;73(1):35–41. doi: 10.1007/BF01286409. [DOI] [PubMed] [Google Scholar]
  5. Johnson K. D., Kende H. Hormonal Control of Lecithin Synthesis in Barley Aleurone Cells: Regulation of the CDP-Choline Pathway by Gibberellin. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2674–2677. doi: 10.1073/pnas.68.11.2674. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. KENNEDY E. P. Biosynthesis of complex lipids. Fed Proc. 1961 Dec;20:934–940. [PubMed] [Google Scholar]
  7. Kagawa T., Lord J. M., Beevers H. The origin and turnover of organelle membranes in castor bean endosperm. Plant Physiol. 1973 Jan;51(1):61–65. doi: 10.1104/pp.51.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lord J. M., Kagawa T., Beevers H. Intracellular distribution of enzymes of the cytidine diphosphate choline pathway in castor bean endosperm. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2429–2432. doi: 10.1073/pnas.69.9.2429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lord J. M., Kagawa T., Moore T. S., Beevers H. Endoplasmic reticulum as the site of lecithin formation in castor bean endosperm. J Cell Biol. 1973 Jun;57(3):659–667. doi: 10.1083/jcb.57.3.659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McCaman R. E., Smith M., Cook K. Intermediary metabolism of phospholipids in brain tissue. II. Phosphatidic acid phosphatase. J Biol Chem. 1965 Sep;240(9):3513–3517. [PubMed] [Google Scholar]
  11. McMurray W. C., Magee W. L. Phospholipid metabolism. Annu Rev Biochem. 1972;41(10):129–160. doi: 10.1146/annurev.bi.41.070172.001021. [DOI] [PubMed] [Google Scholar]
  12. Morré D. J., Nyquist S., Rivera E. Lecithin Biosynthetic Enzymes of Onion Stem and the Distribution of Phosphorylcholine-Cytidyl Transferase among Cell Fractions. Plant Physiol. 1970 Jun;45(6):800–804. doi: 10.1104/pp.45.6.800. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. PAULUS H., KENNEDY E. P. The enzymatic synthesis of inositol monophosphatide. J Biol Chem. 1960 May;235:1303–1311. [PubMed] [Google Scholar]
  14. RACKER E. Spectrophotometric measurements of the enzymatic formation of fumaric and cis-aconitic acids. Biochim Biophys Acta. 1950 Jan;4(1-3):211–214. doi: 10.1016/0006-3002(50)90026-6. [DOI] [PubMed] [Google Scholar]
  15. Raghavan S., Rhoads D., Kanfer J. In vitro incorporation of ( 14 C)serine, ( 14 C)ethanolamine, and ( 14 C)choline into phospholipids of neuronal and glial-enriched fractions from rat brain by base exchange. J Biol Chem. 1972 Nov 25;247(22):7153–7156. [PubMed] [Google Scholar]
  16. Ruby J. R., Dyer R. F., Skalko R. G. Continuities between mitochondria and endoplasmic reticulum in the mammalian ovary. Z Zellforsch Mikrosk Anat. 1969;97(1):30–37. doi: 10.1007/BF00331868. [DOI] [PubMed] [Google Scholar]
  17. Sottocasa G. L., Kuylenstierna B., Ernster L., Bergstrand A. An electron-transport system associated with the outer membrane of liver mitochondria. A biochemical and morphological study. J Cell Biol. 1967 Feb;32(2):415–438. doi: 10.1083/jcb.32.2.415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Vandor S. L., Richardson K. E. Incorporation of ethanolamine-1,2-14C into plant microsomal phospholipids. Can J Biochem. 1968 Oct;46(10):1309–1315. doi: 10.1139/o68-196. [DOI] [PubMed] [Google Scholar]
  19. Vigil E. L. Cytochemical and developmental changes in microbodies (glyoxysomes) and related organelles of castor bean endosperm. J Cell Biol. 1970 Sep;46(3):435–454. doi: 10.1083/jcb.46.3.435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. WILGRAM G. F., KENNEDY E. P. INTRACELLULAR DISTRIBUTION OF SOME ENZYMES CATALYZING REACTIONS IN THE BIOSYNTHESIS OF COMPLEX LIPIDS. J Biol Chem. 1963 Aug;238:2615–2619. [PubMed] [Google Scholar]
  21. Wirtz K. W., Kamp H. H., van Deenen L. L. Isolation of a protein from beef liver which specifically stimulates the exchange of phosphatidylcholine. Biochim Biophys Acta. 1972 Aug 9;274(2):606–617. doi: 10.1016/0005-2736(72)90207-6. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES