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. 1973 Jul;52(1):68–71. doi: 10.1104/pp.52.1.68

Some Aspects of the Regulation of Arginine Biosynthesis in Soybean Cell Cultures 1

P D Shargool a
PMCID: PMC366440  PMID: 16658502

Abstract

The levels of the activities of argininosuccinate synthetase and argininosuccinate lyase were measured in soybean (glycine max L. var. Mandarin) cell suspension cultures grown in the presence or absence of exogenous arginine. In some experiments, actinomycin D or cycloheximide were also added to the cultures, at critical stages of their growth. The results obtained led to the conclusion that activity of argininosuccinate synthetase is subject to significant inhibition by levels of arginine similar to those found to occur within the cells. Argininosuccinate lyase activity appeared to be enhanced, when arginine levels were increased above those occurring physiologically. Both enzymes appeared to be subject to inactivation, possibly via proteolysis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Gamborg O. L., Miller R. A., Ojima K. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res. 1968 Apr;50(1):151–158. doi: 10.1016/0014-4827(68)90403-5. [DOI] [PubMed] [Google Scholar]
  2. Gamborg O. L. The effects of amino acids and ammonium on the growth of plant cells in suspension culture. Plant Physiol. 1970 Apr;45(4):372–375. doi: 10.1104/pp.45.4.372. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  4. Pellizzari E. D., Rising C., Brown J. H., Farmer R. W., Fabre L. F., Jr An improved plasma amino acid purification procedure for gas-liquid chromatography. Anal Biochem. 1971 Nov;44(1):312–316. doi: 10.1016/0003-2697(71)90377-0. [DOI] [PubMed] [Google Scholar]
  5. Reel J. R., Kenney F. T. "Superinduction" of tyrosine transaminase in hepatoma cell cultures: differential inhibition of synthesis and turnover by actionomycin D. Proc Natl Acad Sci U S A. 1968 Sep;61(1):200–206. doi: 10.1073/pnas.61.1.200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. SCHIMKE R. T., SWEENEY E. W., BERLIN C. M. THE ROLES OF SYNTHESIS AND DEGRADATION IN THE CONTROL OF RAT LIVER TRYPTOPHAN PYRROLASE. J Biol Chem. 1965 Jan;240:322–331. [PubMed] [Google Scholar]
  7. Shargool P. D., Cossins E. A. Further studies of L-arginine biosynthesis in germinating pea seeds. Evidence for the presence of argininosuccinate synthetase in cotyledon extracts. Can J Biochem. 1969 Apr;47(4):467–475. doi: 10.1139/o69-073. [DOI] [PubMed] [Google Scholar]
  8. Shargool P. D., Cossins E. A. Isolation and some properties of argininosuccinate lyase from a higher plant source. Can J Biochem. 1968 May;46(5):393–399. doi: 10.1139/o68-060. [DOI] [PubMed] [Google Scholar]
  9. Udaka S. Isolation of the arginine repressor in Escherichia coli. Nature. 1970 Oct 24;228(5269):336–338. doi: 10.1038/228336a0. [DOI] [PubMed] [Google Scholar]
  10. Wampler D. E., Fairley J. L. Argininosuccinate synthetase of Neurospora crassa. Arch Biochem Biophys. 1967 Sep;121(3):580–586. doi: 10.1016/0003-9861(67)90041-0. [DOI] [PubMed] [Google Scholar]

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