Abstract
The effect of arsenate, arsenite, 2,4-dinitrophenol, and anaerobiosis on early events in seed germination was investigated using both intact and punched seeds of lettuce (Lactuca sativa L.). It was found that punching the seed removes penetration barriers to the entrance of inhibitors without an undue loss of germination or light responses. The kinetics of the action of germination inhibitors were established by 2-hour pulse experiments. Arsenate and 2, 4-dinitrophenol have very different kinetics. The inhibition of germination in punched seeds by arsenate given in conjunction with phosphate compared with the lack of inhibition of arsenate plus phosphate on the growing seedling, suggest a distinct metabolic change in the germinating embryo at some time between the onset of germination and subsequent seedling growth.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BUTT W. D., LEES H. The biochemistry of the nitrifying organisms. 6. The effect of oxygen concentration on nitrite oxidation in the presence of different inorganic ions. Biochem J. 1960 Sep;76:425–427. doi: 10.1042/bj0760425. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Haber A. H., Luippold H. J. Separation of Mechanisms Initiating Cell Division and Cell Expansion in Lettuce Seed Germination. Plant Physiol. 1960 Mar;35(2):168–173. doi: 10.1104/pp.35.2.168. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Linschitz H., Kasche V. The kinetics of phytochrome conversion. J Biol Chem. 1966 Jul 25;241(14):3395–3403. [PubMed] [Google Scholar]
- PEERS F. G. The phytase of wheat. Biochem J. 1953 Jan;53(1):102–110. doi: 10.1042/bj0530102. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roux S. J. Chemical evidence for conformational differences between the red- and far-red-absorbing forms of oat phytochrome. Biochemistry. 1972 May 9;11(10):1930–1936. doi: 10.1021/bi00760a030. [DOI] [PubMed] [Google Scholar]
- ter Welle H. F., Slater E. C. Uncoupling of respiratory-chain phosphorylation by arsenate. Biochim Biophys Acta. 1967 Jul 5;143(1):1–17. doi: 10.1016/0005-2728(67)90104-1. [DOI] [PubMed] [Google Scholar]