Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1979 Jan;137(1):409–414. doi: 10.1128/jb.137.1.409-414.1979

Phosphoglucose isomerase mutant of Rhizobium meliloti.

A Arias, C Cerveńansky, A Gardiol, G Martínez-Drets
PMCID: PMC366610  PMID: 762017

Abstract

A mutant strain of complex phenotype was selected in Rhizobium meliloti after nitrosoguanidine mutagenesis. It failed to grow on mannitol, sorbitol, fructose, mannose, ribose, arabitol, or xylose, but grew on glucose, maltose, gluconate, L-arabinose, and many other carbohydrates. Assay showed the enzyme lesion to be in phosphoglucose isomerase (pgi), and revertants, which were of normal growth phenotype, contained the enzyme again. Nonpermissive substrates such as fructose and xylose prevented growth on permissive ones such as L-arabinose, and in such situations there was high accumulation of fructose 6-phosphate. The mutant strain had about 20% as much exopolysaccharide as the parent. Nitrogen fixation by whole plants was low and delayed when the mutant strain was the inoculant.

Full text

PDF
413

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arias A., Martinez-Drets G. Glycerol metabolism in Rhizobium. Can J Microbiol. 1976 Feb;22(2):150–153. doi: 10.1139/m76-020. [DOI] [PubMed] [Google Scholar]
  2. Böck A., Neidhardt F. C. Properties of a Mutant of Escherichia coli with a Temperature-sensitive Fructose-1,6-Diphosphate Aldolase. J Bacteriol. 1966 Aug;92(2):470–476. doi: 10.1128/jb.92.2.470-476.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Duncan M. J., Fraenkel D. G. alpha-Ketoglutarate dehydrogenase mutant of Rhizobium meliloti. J Bacteriol. 1979 Jan;137(1):415–419. doi: 10.1128/jb.137.1.415-419.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. FRAENKEL D. G., HORECKER B. L. PATHWAYS OF D-GLUCOSE METABOLISM IN SALMONELLA TYPHINMURIUM. A STUDY OF A MUTANT LACKING PHOSPHOGLUCOSE ISOMERASE. J Biol Chem. 1964 Sep;239:2765–2771. [PubMed] [Google Scholar]
  5. Fradkin J. E., Fraenkel D. G. 2-keto-3-deoxygluconate 6-phosphate aldolase mutants of Escherichia coli. J Bacteriol. 1971 Dec;108(3):1277–1283. doi: 10.1128/jb.108.3.1277-1283.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fraenkel D. G. Selection of Escherichia coli mutants lacking glucose-6-phosphate dehydrogenase or gluconate-6-phosphate dehydrogenase. J Bacteriol. 1968 Apr;95(4):1267–1271. doi: 10.1128/jb.95.4.1267-1271.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fraenkel D. G. The accumulation of glucose 6-phosphate from glucose and its effect in an Escherichia coli mutant lacking phosphoglucose isomerase and glucose 6-phosphate dehydrogenase. J Biol Chem. 1968 Dec 25;243(24):6451–6457. [PubMed] [Google Scholar]
  8. Keele B. B., Jr, Hamilton P. B., Elkan G. H. Gluconate catabolism in Rhizobium japonicum. J Bacteriol. 1970 Mar;101(3):698–704. doi: 10.1128/jb.101.3.698-704.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Keele B. B., Jr, Hamilton P. B., Elkan G. H. Glucose catabolism in Rhizobium japonicum. J Bacteriol. 1969 Mar;97(3):1184–1191. doi: 10.1128/jb.97.3.1184-1191.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Martinez De Drets G., Arias A. Metabolism of some polyols by Rhizobium meliloti. J Bacteriol. 1970 Jul;103(1):97–103. doi: 10.1128/jb.103.1.97-103.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Martinez-de Drets G., Arias A., Rovira de Cutinella M. Fast- and slow-growing rhizobia: differences in sucrose utilization and invertase activity. Can J Microbiol. 1974 Apr;20(4):605–609. doi: 10.1139/m74-092. [DOI] [PubMed] [Google Scholar]
  12. Martínez-De Drets G., Arias A. Enzymatic basis for differentiation of Rhizobium into fast- and slow-growing groups. J Bacteriol. 1972 Jan;109(1):467–470. doi: 10.1128/jb.109.1.467-470.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mulongoy K., Elkan G. H. Glucose catabolism in two derivatives of a Rhizobium japonicum strain differing in nitrogen-fixing efficiency. J Bacteriol. 1977 Jul;131(1):179–187. doi: 10.1128/jb.131.1.179-187.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nutman P. S. Variation within Strains of Clover Nodule Bacteria in Size of Nodule Produced and in "Effectivity" of Symbiosis. J Bacteriol. 1946 Apr;51(4):411–432. [PMC free article] [PubMed] [Google Scholar]
  15. Phibbs P. V., Jr, McCowen S. M., Feary T. W., Blevins W. T. Mannitol and fructose catabolic pathways of Pseudomonas aeruginosa carbohydrate-negative mutants and pleiotropic effects of certain enzyme deficiencies. J Bacteriol. 1978 Feb;133(2):717–728. doi: 10.1128/jb.133.2.717-728.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES