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. 1974 Dec;54(6):950–952. doi: 10.1104/pp.54.6.950

Autocatalysis in a Reconstituted Chloroplast System 1

David A Walker a, Ross McC Lilley a
PMCID: PMC366639  PMID: 16659006

Abstract

In whole plants and intact chloroplasts, photosynthesis does not reach its full rate immediately upon illumination but only after a lag which is believed to reflect an autocatalytic increase in the concentration of carbon cycle intermediates. Autocatalysis has now been observed in a reconstituted system containing envelope-free chloroplasts augmented with ferredoxin and other stromal proteins but only catalytic amounts of ATP and NADP. With ribose 5-phosphate as substrate, the CO2 dependent O2 evolution recorded for such mixtures implies rates of “endogenous” or ferredoxin-dependent photophosphorylation as high as 360 μmoles of orthophosphate esterified mg−1 chlorophyll hr−1.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnon D. I., Tsujimoto H. Y., McSwain B. D. Ferredoxin and photosynthetic phosphorylation. Nature. 1967 May 6;214(5088):562–566. doi: 10.1038/214562a0. [DOI] [PubMed] [Google Scholar]
  2. Avron M., Gibbs M. Carbon dioxide fixation in the light and in the dark by isolated spinach chloroplasts. Plant Physiol. 1974 Feb;53(2):140–143. doi: 10.1104/pp.53.2.140. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bahr J. T., Jensen R. G. Ribulose Diphosphate Carboxylase from Freshly Ruptured Spinach Chloroplasts Having an in Vivo Km[CO(2)]. Plant Physiol. 1974 Jan;53(1):39–44. doi: 10.1104/pp.53.1.39. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cockburn W., Walker D. A., Baldry C. W. Photosynthesis by isolated chloroplasts. Reversal of orthophosphate inhibition by Calvin-cycle intermediates. Biochem J. 1968 Mar;107(1):89–95. doi: 10.1042/bj1070089. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Haslett B. G., Cammack R., Whatley F. R. Quantitative studies on ferredoxin in greening bean leaves. Biochem J. 1973 Nov;136(3):697–703. doi: 10.1042/bj1360697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Walker D. A., McCormick A. V., Stokes D. M. CO 2 -dependent oxygen evolution by envelope-free chloroplasts. Nature. 1971 Oct 1;233(5318):346–347. doi: 10.1038/233346a0. [DOI] [PubMed] [Google Scholar]

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