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. 1974 Oct;54(4):484–486. doi: 10.1104/pp.54.4.484

Fat Metabolism in Higher Plants

LXII. Stearl-acyl Carrier Protein Desaturase from Spinach Chloroplasts 1

Bruce S Jacobson a,2, J G Jaworski a, P K Stumpf a
PMCID: PMC367438  PMID: 16658913

Abstract

Stearyl-acyl carrier protein desaturase (EC 1.14.99.6), present in the stroma fraction of spinach (Spinacia oleracea) chloroplasts, rapidly desaturated enzymatically prepared stearyl-acyl carrier protein to oleic acid. No other substrates were desaturated. In addition to stearyl-acyl carrier protein, reduced ferredoxin was an essential component of the system. The electron donor systems were either ascorbate, dichlorophenolindophenol, photosystem I and light, or NADPH and ferredoxin-NADP reductase. The desaturase was more active in extracts prepared from chloroplasts obtained from immature spinach leaves than from mature leaves. Stearyl-acyl carrier protein desaturase also occurs in soluble extracts of avocado (Persea americana Mill.) mesocarp and of developing safflower (Carthamus tinctorius) seeds.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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