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. 1984 Jan;4(1):86–91. doi: 10.1128/mcb.4.1.86

Isolation and characterization of the centromere from chromosome V (CEN5) of Saccharomyces cerevisiae.

G T Maine, R T Surosky, B K Tye
PMCID: PMC368661  PMID: 6366514

Abstract

We have cloned a functional centromeric DNA sequence from Saccharomyces cerevisiae. Using the 2 mu chromosome-loss mapping technique and meiotic tetrad analysis, we have identified this DNA sequence as the centromere of chromosome V (CEN5). The CEN5 sequence has been localized on an 1,100-base-pair BamHI-BglII restriction fragment. Plasmids containing CEN5 and an autonomously replicating sequence are mitotically stable in S. cerevisiae and segregate in a Mendelian fashion during meiosis.

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Selected References

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