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. 1984 Nov;4(11):2540–2542. doi: 10.1128/mcb.4.11.2540

Transcriptional regulation of a tumor promoter and mitogen-inducible gene in human lymphocytes.

S K Arya, F Wong-Staal, R C Gallo
PMCID: PMC369087  PMID: 6513930

Abstract

Tumor-promoting phorbol esters affect a variety of cellular functions which may underlie tumor promotion. We isolated from human lymphocytes a cDNA clone whose gene is inducible by the phorbol ester 12-O-tetradecanoyl-phorbol-13-acetate as well as by the T-cell mitogen phytohemagglutinin. Nuclear transcription experiments suggested that this induction is primarily caused by the increased transcription of the gene. It is interesting that this gene is expressed constitutively in human T-cell leukemia virus-infected mature T cells. The results support the notion that 12-O-tetradecanoyl-phorbol-13-acetate can affect cellular functions by causing transcriptional activation of specific genes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arya S. K. Phorbol ester-mediated stimulation of the synthesis of mouse mammary tumour virus. Nature. 1980 Mar 6;284(5751):71–72. doi: 10.1038/284071a0. [DOI] [PubMed] [Google Scholar]
  2. Arya S. K. Sequence complexity of polyadenylated RNA in human breast carcinoma cells. Int J Biochem. 1982;14(1):19–24. doi: 10.1016/0020-711x(82)90171-9. [DOI] [PubMed] [Google Scholar]
  3. Arya S. K., Wong-Staal F., Gallo R. C. T-cell growth factor gene: lack of expression in human T-cell leukemia-lymphoma virus-infected cells. Science. 1984 Mar 9;223(4640):1086–1087. doi: 10.1126/science.6320374. [DOI] [PubMed] [Google Scholar]
  4. Blumberg P. M. In vitro studies on the mode of action of the phorbol esters, potent tumor promoters: part 1. Crit Rev Toxicol. 1980 Dec;8(2):153–197. doi: 10.3109/10408448009037493. [DOI] [PubMed] [Google Scholar]
  5. Boutwell R. K., Verma A. K., Ashendel C. L., Astrup E. Mouse skin: a useful model system for studying the mechanism of chemical carcinogenesis. Carcinog Compr Surv. 1982;7:1–12. [PubMed] [Google Scholar]
  6. Chao M. V., Mellon P., Charnay P., Maniatis T., Axel R. The regulated expression of beta-globin genes introduced into mouse erythroleukemia cells. Cell. 1983 Feb;32(2):483–493. doi: 10.1016/0092-8674(83)90468-3. [DOI] [PubMed] [Google Scholar]
  7. Clark S. C., Arya S. K., Wong-Staal F., Matsumoto-Kobayashi M., Kay R. M., Kaufman R. J., Brown E. L., Shoemaker C., Copeland T., Oroszlan S. Human T-cell growth factor: partial amino acid sequence, cDNA cloning, and organization and expression in normal and leukemic cells. Proc Natl Acad Sci U S A. 1984 Apr;81(8):2543–2547. doi: 10.1073/pnas.81.8.2543. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cochran B. H., Reffel A. C., Stiles C. D. Molecular cloning of gene sequences regulated by platelet-derived growth factor. Cell. 1983 Jul;33(3):939–947. doi: 10.1016/0092-8674(83)90037-5. [DOI] [PubMed] [Google Scholar]
  9. Fibach E., Gambari R., Shaw P. A., Maniatis G., Reuben R. C., Sassa S., Rifkind R. A., Marks P. A. Tumor promoter-mediated inhibition of cell differentiation: suppression of the expression of erythroid functions in murine erythroleukemia cells. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1906–1910. doi: 10.1073/pnas.76.4.1906. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gottesman M. M., Sobel M. E. Tumor promoters and Kirsten sarcoma virus increase synthesis of a secreted glycoprotein by regulating levels of translatable mRNA. Cell. 1980 Feb;19(2):449–455. doi: 10.1016/0092-8674(80)90519-x. [DOI] [PubMed] [Google Scholar]
  11. Groudine M., Peretz M., Weintraub H. Transcriptional regulation of hemoglobin switching in chicken embryos. Mol Cell Biol. 1981 Mar;1(3):281–288. doi: 10.1128/mcb.1.3.281. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hiwasa T., Fujimura S., Sakiyama S. Tumor promoters increase the synthesis of a 32,000-dalton protein in BALB/c 3T3 cells. Proc Natl Acad Sci U S A. 1982 Mar;79(6):1800–1804. doi: 10.1073/pnas.79.6.1800. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kelly K., Cochran B. H., Stiles C. D., Leder P. Cell-specific regulation of the c-myc gene by lymphocyte mitogens and platelet-derived growth factor. Cell. 1983 Dec;35(3 Pt 2):603–610. doi: 10.1016/0092-8674(83)90092-2. [DOI] [PubMed] [Google Scholar]
  14. Linzer D. I., Nathans D. Growth-related changes in specific mRNAs of cultured mouse cells. Proc Natl Acad Sci U S A. 1983 Jul;80(14):4271–4275. doi: 10.1073/pnas.80.14.4271. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. McKnight G. S., Palmiter R. D. Transcriptional regulation of the ovalbumin and conalbumin genes by steroid hormones in chick oviduct. J Biol Chem. 1979 Sep 25;254(18):9050–9058. [PubMed] [Google Scholar]
  16. Miao R. M., Filedsteel A. H., Fodge D. W. Opposing effects of tumor promoters on erythroid differentiation. Nature. 1978 Jul 20;274(5668):271–272. doi: 10.1038/274271a0. [DOI] [PubMed] [Google Scholar]
  17. Mulvihill E. R., Palmiter R. D. Relationship of nuclear estrogen receptor levels to induction of ovalbumin and conalbumin mRNA in chick oviduct. J Biol Chem. 1977 Mar 25;252(6):2060–2068. [PubMed] [Google Scholar]
  18. Rovera G., O'Brien T. G., Diamond L. Tumor promoters inhibit spontaneous differentiation of Friend erythroleukemia cells in culture. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2894–2898. doi: 10.1073/pnas.74.7.2894. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rovera G., Santoli D., Damsky C. Human promyelocytic leukemia cells in culture differentiate into macrophage-like cells when treated with a phorbol diester. Proc Natl Acad Sci U S A. 1979 Jun;76(6):2779–2783. doi: 10.1073/pnas.76.6.2779. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Scribner J. D., Süss R. Tumor initiation and promotion. Int Rev Exp Pathol. 1978;18:137–198. [PubMed] [Google Scholar]
  21. Sobel M. E., Dion L. D., Vuust J., Colburn N. H. Tumor-promoting phorbol esters inhibit procollagen synthesis at a pretranslational level in JB-6 mouse epidermal cells. Mol Cell Biol. 1983 Aug;3(8):1527–1532. doi: 10.1128/mcb.3.8.1527. [DOI] [PMC free article] [PubMed] [Google Scholar]

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