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. 1981 Jul;1(7):629–634. doi: 10.1128/mcb.1.7.629

Transcriptional regulation of the Kluyveromyces lactis beta-galactosidase gene.

L R Lacy 1, R C Dickson 1
PMCID: PMC369710  PMID: 9279376

Abstract

We examined the molecular basis for beta-D-galactosidase (EC 3.2.1.23) induction in the yeast Kluyveromyces lactis. The protein synthesis inhibitor anisomycin effectively blocked both protein synthesis and enzyme induction by lactose. Further, hybridization analysis with the cloned beta-galactosidase gene indicated coordinate increases in the concentration of beta-galactosidase messenger ribonucleic acid and enzyme activity. The half-life of beta-galactosidase messenger ribonucleic acid was the same (4.8 +/- 0.4 min) when measured both before and at succeeding times during enzyme induction. These results strongly support the hypothesis that expression of the yeast beta-galactosidase gene is subject to transcriptional regulation.

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Selected References

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