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. 1983 Sep;3(9):1552–1561. doi: 10.1128/mcb.3.9.1552

Changes in liver-specific compared to common gene transcription during primary culture of mouse hepatocytes.

D F Clayton, J E Darnell Jr
PMCID: PMC370008  PMID: 6633533

Abstract

Liver-specific mRNA sequences were examined in primary cultures of mouse hepatocytes. After cell disaggregation by collagenase treatment and for at least 24 h in culture, little change in liver-specific mRNA concentrations was noted. Gradually over a period of 140 h, liver-specific mRNAs declined. In contrast, transcriptional assays in which liver cell nuclei were used to produce 32P-labeled nuclear RNA showed that liver-specific gene transcription was greatly diminished within 24 h, while polymerase II transcription of "common" genes and transcription of tRNA and rRNA did not decline. Thus, a prompt differential transcriptional effect seems to underlie the gradual loss of tissue specificity of the primary cultures.

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Selected References

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