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. 1983 Jul;3(7):1310–1316. doi: 10.1128/mcb.3.7.1310

Immunological selection of variant mouse lymphoid cells with altered glucocorticoid responsiveness.

M Danielsen, D O Peterson, M R Stallcup
PMCID: PMC370121  PMID: 6310372

Abstract

We have devised an immunological procedure to separate cells on the basis of expression of mouse mammary tumor virus (MMTV) gene products. Plastic petri dishes coated with specific antibodies against MMTV proteins bind cells with an efficiency that correlates with the level of MMTV gene expression. Glucocorticoid-sensitive mouse thymoma cell line W7 was infected with MMTV. Clones from the infected population retain the relatively slow cytolytic glucocorticoid response and, in addition, exhibit a rapid induction of MMTV-specific RNA and proteins. By combining our immunological selection with the selection for resistance to hormone-mediated cytolysis, we have isolated variant cells which are resistant to the cytotoxic effect of glucocorticoids but which retain the induction of viral gene products and must therefore have a functional glucocorticoid receptor protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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