Skip to main content
PMC home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1982 Jun;69(6):1393–1397. doi: 10.1172/JCI110580

Inhibition of neutrophil lysosome-phagosome fusion associated with influenza virus infection in vitro. Role in depressed bactericidal activity.

J S Abramson, J C Lewis, D S Lyles, K A Heller, E L Mills, D A Bass
PMCID: PMC370213  PMID: 7085879

Abstract

The present study examined the effect of various unopsonized strains of influenza A virus on release of myeloperoxidase (MPO) and acid phosphatase in polymorphonuclear leukocytes (PMNL). These results were correlated with the effect that these same viruses had on bactericidal activity in PMNL. Several strains of virus inhibited the fusion of azurophil granules with phagosomes containing Staphylococcus aureus. These same strains inhibited the extracellular release of MPO from PMNL (39-59%) and caused depressed killing (42-77%). In contrast, one of the influenza viruses (X-47a) did not inhibit PMNL MPO release or killing. The data indicate a close relationship between the ability of influenza virus to ablate normal intracellular lysosome-phagosome fusion with subsequent depression of bactericidal functions of PMNL.

Full text

PDF
1395

Images in this article

1394Image
on p.1394

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abramson J. S., Giebink G. S., Mills E. L., Quie P. G. Polymorphonuclear leukocyte dysfunction during influenza virus infection in chinchillas. J Infect Dis. 1981 Jun;143(6):836–845. doi: 10.1093/infdis/143.6.836. [DOI] [PubMed] [Google Scholar]
  2. Abramson J. S., Mills E. L., Giebink G. S., Quie P. G. Depression of monocyte and polymorphonuclear leukocyte oxidative metabolism and bactericidal capacity by influenza A virus. Infect Immun. 1982 Jan;35(1):350–355. doi: 10.1128/iai.35.1.350-355.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bass D. A., Lewis J. C., Szejda P., Cowley L., McCall C. E. Activation of lysosomal acid phosphatase of eosinophil leukocytes. Lab Invest. 1981 May;44(5):403–409. [PubMed] [Google Scholar]
  4. Breton-Gorius J., Coquin Y., Guichard J. Cytochemical distinction between azurophils and catalase-containing granules in leukocytes. I. Studies in developing neutrophils and monocytes from patients with myeloperoxidase deficiency: comparison with peroxidase-deficient chicken heterophils. Lab Invest. 1978 Jan;38(1):21–31. [PubMed] [Google Scholar]
  5. Caliguiri L. A., Klenk H. D., Choppin P. W. The proteins of the parainfluenza virus SV5. 1. Separation of virion polypeptides by polyacrylamide gel electrophoresis. Virology. 1969 Nov;39(3):460–466. doi: 10.1016/0042-6822(69)90094-4. [DOI] [PubMed] [Google Scholar]
  6. Couch R. B. The effects of influenza on host defenses. J Infect Dis. 1981 Sep;144(3):284–291. doi: 10.1093/infdis/144.3.284. [DOI] [PubMed] [Google Scholar]
  7. Graham R. C., Jr, Karnovsky M. J. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem. 1966 Apr;14(4):291–302. doi: 10.1177/14.4.291. [DOI] [PubMed] [Google Scholar]
  8. Jakab G. J., Warr G. A., Knight M. E. Pulmonary and systemic defenses against challenge with Staphylococcus aureus in mice with pneumonia due to influenza A virus. J Infect Dis. 1979 Jul;140(1):105–108. doi: 10.1093/infdis/140.1.105. [DOI] [PubMed] [Google Scholar]
  9. Jakab G. J., Warr G. A., Sannes P. L. Alveolar macrophage ingestion and phagosome-lysosome fusion defect associated with virus pneumonia. Infect Immun. 1980 Mar;27(3):960–968. doi: 10.1128/iai.27.3.960-968.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Matlin K. S., Reggio H., Helenius A., Simons K. Infectious entry pathway of influenza virus in a canine kidney cell line. J Cell Biol. 1981 Dec;91(3 Pt 1):601–613. doi: 10.1083/jcb.91.3.601. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mills E. L., Debets-Ossenkopp Y., Verbrugh H. A., Verhoef J. Initiation of the respiratory burst of human neutrophils by influenza virus. Infect Immun. 1981 Jun;32(3):1200–1205. doi: 10.1128/iai.32.3.1200-1205.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Oxford J. S., McGeoch D. J., Schild G. C., Beare A. S. Analysis of virion RNA segments and polypeptides of influenza A virus recombinants of defined virulence. Nature. 1978 Jun 29;273(5665):778–779. doi: 10.1038/273778a0. [DOI] [PubMed] [Google Scholar]
  14. Quie P. G., White J. G., Holmes B., Good R. A. In vitro bactericidal capacity of human polymorphonuclear leukocytes: diminished activity in chronic granulomatous disease of childhood. J Clin Invest. 1967 Apr;46(4):668–679. doi: 10.1172/JCI105568. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Root R. K., Cohen M. S. The microbicidal mechanisms of human neutrophils and eosinophils. Rev Infect Dis. 1981 May-Jun;3(3):565–598. doi: 10.1093/clinids/3.3.565. [DOI] [PubMed] [Google Scholar]
  16. Scheid A., Choppin P. W. Identification of biological activities of paramyxovirus glycoproteins. Activation of cell fusion, hemolysis, and infectivity of proteolytic cleavage of an inactive precursor protein of Sendai virus. Virology. 1974 Feb;57(2):475–490. doi: 10.1016/0042-6822(74)90187-1. [DOI] [PubMed] [Google Scholar]
  17. Silverberg B. A., Jakab G. J., Thomson R. G., Warr G. A., Boo K. S. Ultrastructural alterations in phagocytic functions of alveolar macrophages after parainfluenza virus infection. J Reticuloendothel Soc. 1979 Apr;25(4):405–416. [PubMed] [Google Scholar]
  18. Stevens P., Winston D. J., Van Dyke K. In vitro evaluation of opsonic and cellular granulocyte function by luminol-dependent chemiluminescence: utility in patients with severe neutropenia and cellular deficiency states. Infect Immun. 1978 Oct;22(1):41–51. doi: 10.1128/iai.22.1.41-51.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Warshauer D., Goldstein E., Akers T., Lippert W., Kim M. Effect of influenza viral infection on the ingestion and killing of bacteria by alveolar macrophages. Am Rev Respir Dis. 1977 Feb;115(2):269–277. doi: 10.1164/arrd.1977.115.2.269. [DOI] [PubMed] [Google Scholar]
  20. Williams A. J., Cole P. J. The onset of polymorphonuclear leucocyte membrane-stimulated metabolic activity. Immunology. 1981 Aug;43(4):733–739. [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES