Skip to main content
PMC home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1982 Oct;70(4):798–805. doi: 10.1172/JCI110676

Mesenchyme-epithelial interactions in human endometrium. Prostaglandin synthesis in separated cell types.

D Gal, M L Casey, J M Johnston, P C MacDonald
PMCID: PMC370288  PMID: 6811614

Abstract

Glandular epithelium and stromal cells of human endometrium were separated and maintained in monolayer culture. At the time the cells became confluent, cell suspensions were prepared and incubated with [14C]arachidonic acid. Radiolabeled prostaglandin E2 and, to a lesser extent, prostaglandin F2 alpha and metabolites of these prostaglandins, were formed principally in stromal cells. There was considerably less prostaglandin formation in endometrial glands either after maintenance in monolayer culture or in freshly separated glands. In stromal cells of endometrium prostaglandin formation was linear with time of incubation for 2.5 min and with [14C]arachidonic acid concentrations up to 8 microM. When stromal cells and epithelial cells were combined, all prostaglandin formation could be accounted for by that produced in stromal cells. Little or no prostaglandin formation was detected in stromal cells from human adipose tissue or in fibroblasts from human genital or abdominal skin or human fallopian tube.

Full text

PDF
800

Images in this article

800Image
on p.800

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abel M. H., Baird D. T. The effect of 17 beta-estradiol and progesterone on prostaglandin production by human endometrium maintained in organ culture. Endocrinology. 1980 May;106(5):1599–1606. doi: 10.1210/endo-106-5-1599. [DOI] [PubMed] [Google Scholar]
  2. Ackerman G. E., Smith M. E., Mendelson C. R., MacDonald P. C., Simpson E. R. Aromatization of androstenedione by human adipose tissue stromal cells in monolayer culture. J Clin Endocrinol Metab. 1981 Aug;53(2):412–417. doi: 10.1210/jcem-53-2-412. [DOI] [PubMed] [Google Scholar]
  3. Casey M. L., Hemsell D. L., MacDonald P. C., Johnston J. M. NAD+-dependent 15-hydroxyprostaglandin dehydrogenase activity in human endometrium. Prostaglandins. 1980 Jan;19(1):115–122. doi: 10.1016/0090-6980(80)90159-8. [DOI] [PubMed] [Google Scholar]
  4. Coene M. C., Van Hove C., Claeys M., Herman A. G. Arachidonic acid metabolism by cultured mesothelial cells. Different transformations of exogenously added and endogenously. Biochim Biophys Acta. 1982 Mar 12;710(3):437–445. doi: 10.1016/0005-2760(82)90127-8. [DOI] [PubMed] [Google Scholar]
  5. Cunha G. R., Chung L. W., Shannon J. M., Reese B. A. Stromal-epithelial interactions in sex differentiation. Biol Reprod. 1980 Feb;22(1):19–42. doi: 10.1095/biolreprod22.1.19. [DOI] [PubMed] [Google Scholar]
  6. DeCosse J. J., Gossens C. L., Kuzma J. F., Unsworth B. R. Breast cancer: induction of differentiation by embryonic tissue. Science. 1973 Sep 14;181(4104):1057–1058. doi: 10.1126/science.181.4104.1057. [DOI] [PubMed] [Google Scholar]
  7. Douarin N. M. An experimental analysis of liver development. Med Biol. 1975 Dec;53(6):427–455. [PubMed] [Google Scholar]
  8. Erman A., Azuri R., Raz A. Different pools of esterified arachidonic acid in rabbit kidney medulla: relationship to Ca2+-stimulated prostaglandin biosynthesis. Lipids. 1982 Mar;17(3):119–123. doi: 10.1007/BF02535090. [DOI] [PubMed] [Google Scholar]
  9. Ham E. A., Cirillo V. J., Zanetti M. E., Kuehl F. A., Jr Estrogen-directed synthesis of specific prostaglandins in uterus. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1420–1424. doi: 10.1073/pnas.72.4.1420. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Liggins G. C., Fairclough R. J., Grieves S. A., Kendall J. Z., Knox B. S. The mechanism of initiation of parturition in the ewe. Recent Prog Horm Res. 1973;29:111–159. doi: 10.1016/b978-0-12-571129-6.50007-5. [DOI] [PubMed] [Google Scholar]
  12. Maathuis J. B., Kelly R. W. Concentrations of prostaglandins F2alpha and E2 in the endometrium throughout the human menstrual cycle, after the administration of clomiphene or an oestrogen-progestogen pill and in early pregnancy. J Endocrinol. 1978 Jun;77(3):361–371. doi: 10.1677/joe.0.0770361. [DOI] [PubMed] [Google Scholar]
  13. MacDonald P. C., Porter J. C., Schwarz B. E., Johnston J. M. Initiation of parturition in the human female. Semin Perinatol. 1978 Jul;2(3):273–286. [PubMed] [Google Scholar]
  14. Milewich L., Gomez-Sanchez C., Madden J. D., MacDonald P. C. Isolation and characterization of 5alpha-pregnane-3,20-dione and progesterone in pepipheral blood of pregnant women. measurement throughout pregnancy. Gynecol Invest. 1975;6(5):291–306. [PubMed] [Google Scholar]
  15. Satyaswaroop P. G., Bressler R. S., de la Pena M. M., Gurpide E. Isolation and culture of human endometrial glands. J Clin Endocrinol Metab. 1979 Apr;48(4):639–641. doi: 10.1210/jcem-48-4-639. [DOI] [PubMed] [Google Scholar]
  16. Singh E. J., Baccarini I., Zuspan F. P. Levels of prostaglandins F-2alpha and E-2 in human endometrium during the menstrual cycle. Am J Obstet Gynecol. 1975 Apr 1;121(7):1003–1006. doi: 10.1016/0002-9378(75)90927-8. [DOI] [PubMed] [Google Scholar]
  17. Spooner B. S., Wessells N. K. Mammalian lung development: interactions in primordium formation and bronchial morphogenesis. J Exp Zool. 1970 Dec;175(4):445–454. doi: 10.1002/jez.1401750404. [DOI] [PubMed] [Google Scholar]
  18. Wilson J. D. Dihydrotestosterone formation in cultured human fibroblasts. Comparison of cells from normal subjects and patients with familial incomplete male pseudohermaphroditism, Type 2. J Biol Chem. 1975 May 10;250(9):3498–3504. [PubMed] [Google Scholar]
  19. Wright K., Collins D. C., Preedy J. R. Comparative specificity of antisera raised against estrone, estradiol-17 and estriol using 6-0-carboxy-methyloxime bovine serum albumin derivatives. Steroids. 1973 May;21(5):755–769. doi: 10.1016/0039-128x(73)90140-2. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES