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. 1981 May;67(5):1580–1583. doi: 10.1172/JCI110190

Epidermal growth factor receptor number decreases during rat liver regeneration.

H S Earp, E J O'Keefe
PMCID: PMC370728  PMID: 6262381

Abstract

The potential role of epidermal growth factor (EGF) in the regulation of rat liver regeneration was examined by assessing the binding of 125I-EGF to hepatic membranes isolated at various times after partial hepatectomy. The results demonstrated a fall in 125I-EGF binding detectable as early as 8 h after partial hepatectomy. The nadir in EGF binding, less than 40% of that observed in sham-operated control rats, was seen 36 and 48 h after partial hepatectomy. Scatchard analysis showed that the decrease in binding capacity was due to a fall in receptor number. The specificity of the observed loss of EGF receptors was substantiated in parallel studies of 125I-insulin and 125I-wheat germ lectin binding; the binding of these ligands did not decrease appreciably during liver regeneration. The data are consistent with the hypothesis that EGF or a similar substance is one component of the complex humoral signal that regulates liver regeneration.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baker J. B., Barsh G. S., Carney D. H., Cunningham D. D. Dexamethasone modulates binding and action of epidermal growth factor in serum-free cell culture. Proc Natl Acad Sci U S A. 1978 Apr;75(4):1882–1886. doi: 10.1073/pnas.75.4.1882. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blomberg J., Reynolds F. H., Jr, Van de Ven W. J., Stephenson J. R. Abelson murine leukaemia virus transformation involves loss of epidermal growth factor-binding sites. Nature. 1980 Jul 31;286(5772):504–507. doi: 10.1038/286504a0. [DOI] [PubMed] [Google Scholar]
  3. Bucher N. L., Patel U., Cohen S. Hormonal factors and liver growth. Adv Enzyme Regul. 1977 Oct 3;16:205–213. doi: 10.1016/0065-2571(78)90074-2. [DOI] [PubMed] [Google Scholar]
  4. Bucher N. L., Swaffield M. N. Synergistic action of glucagon and insulin in regulation of hepatic regeneration. Adv Enzyme Regul. 1975;13:281–293. [PubMed] [Google Scholar]
  5. Carpenter G., Cohen S. Epidermal growth factor. Annu Rev Biochem. 1979;48:193–216. doi: 10.1146/annurev.bi.48.070179.001205. [DOI] [PubMed] [Google Scholar]
  6. Das M., Fox C. F. Molecular mechanism of mitogen action: processing of receptor induced by epidermal growth factor. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2644–2648. doi: 10.1073/pnas.75.6.2644. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
  8. Heimberg M., Weinstein I., LeQuire V. S., Cohen S. The induction of fatty liver in neonatal animals by a purified protein (EGF) from mouse submaxillary gland. Life Sci. 1965 Sep;4(17):1625–1633. doi: 10.1016/0024-3205(65)90206-7. [DOI] [PubMed] [Google Scholar]
  9. Herschman H. R., Lusis A. J., Groopman J. E. Growth factors. Ann Intern Med. 1980 May;92(5):650–662. doi: 10.7326/0003-4819-92-5-650. [DOI] [PubMed] [Google Scholar]
  10. Hollenberg M. D. Epidermal growth factor-urogastrone, a polypeptide acquiring hormonal status. Vitam Horm. 1979;37:69–110. doi: 10.1016/s0083-6729(08)61068-7. [DOI] [PubMed] [Google Scholar]
  11. Koide Y., Earp H. S., Ong S. H., Steiner A. L. Alterations in the intracellular distribution of cGMP and guanylate cyclase activity during rat liver regeneration. J Biol Chem. 1978 Jun 25;253(12):4439–4445. [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. O'Keefe E., Hollenberg M. D., Cuatrecasas P. Epidermal growth factor. Characteristics of specific binding in membranes from liver, placenta, and other target tissues. Arch Biochem Biophys. 1974 Oct;164(2):518–526. doi: 10.1016/0003-9861(74)90062-9. [DOI] [PubMed] [Google Scholar]
  14. Richman R. A., Claus T. H., Pilkis S. J., Friedman D. L. Hormonal stimulation of DNA synthesis in primary cultures of adult rat hepatocytes. Proc Natl Acad Sci U S A. 1976 Oct;73(10):3589–3593. doi: 10.1073/pnas.73.10.3589. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Savage C. R., Jr, Cohen S. Proliferation of corneal epithelium induced by epidermal growth factor. Exp Eye Res. 1973 Mar;15(3):361–366. doi: 10.1016/0014-4835(73)90151-6. [DOI] [PubMed] [Google Scholar]
  16. Todaro G. J., De Larco J. E., Cohen S. Transformation by murine and feline sarcoma viruses specifically blocks binding of epidermal growth factor to cells. Nature. 1976 Nov 4;264(5581):26–31. doi: 10.1038/264026a0. [DOI] [PubMed] [Google Scholar]
  17. Touster O., Aronson N. N., Jr, Dulaney J. T., Hendrickson H. Isolation of rat liver plasma membranes. Use of nucleotide pyrophosphatase and phosphodiesterase I as marker enzymes. J Cell Biol. 1970 Dec;47(3):604–618. doi: 10.1083/jcb.47.3.604. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wrann M., Fox C. F., Ross R. Modulation of epidermal growth factor receptors on 3T3 cells by platelet-derived growth factor. Science. 1980 Dec 19;210(4476):1363–1365. doi: 10.1126/science.6254158. [DOI] [PubMed] [Google Scholar]

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