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. 1980 Feb;65(2):506–513. doi: 10.1172/JCI109694

Neuromorphology and Neuropharmacology of the Human Penis

AN IN VITRO STUDY

George S Benson 1,2, Joann McConnell 1,2, Larry I Lipshultz 1,2, Joseph N Corriere Jr 1,2, Joe Wood 1,2
PMCID: PMC371389  PMID: 7356692

Abstract

The neuromorphology and neuropharmacology of the human penis are only briefly described in literature. The present study was undertaken to define the adrenergic and cholinergic neuromorphology of the human corpus cavernosum (CC) and corpus spongiosum and to evaluate the in vitro response of the CC to pharmacologic stimulation. Human penile tissue was obtained from six transsexual patients undergoing penectomy. For morphologic study, the tissue was processed for (a) hematoxylin and eosin staining; (b) smooth muscle staining; (c) acetylcholinesterase localization; (d) glyoxylic acid histofluorescence; (e) electron microscopy; and (f) electron microscopy after glutaraldehyde dichromate fixation. In addition, strips of CC were placed in in vitro muscle chambers and tension changes recorded isometrically after stimulation with norepinephrine (NE) and acetylcholine. The CC contains abundant smooth muscle, numerous glyoxylic acidfluorescent (catecholaminergic) fibers and varicosities, and a scant distribution of acetylcholinesterase-positive fibers. Fewer of all these elements were present in the corpus spongiosum. No “polsters” were observed in the CC. Although glutaraldehyde-fixed controls exhibited no typical adrenergic vesicles (small, dense core, measuring 400-600 Å in diameter), some small, strongly electron-dense vesicles were found in glutaraldehyde dichromate-fixed tissue and were thought to contain NE. A variety of other vesicles were also encountered. The addition of NE to the in vitro muscle chambers caused a dose-related contraction, which was blocked by pretreatment with phentolamine in all CC strips tested. Acetylcholine in high concentration produced minimal contraction in 2 of 24 strips. Our morphologic and pharmacologic data suggest that the sympathetic nervous system may affect erection by acting not only on the penile vasculature but also by direct action on the smooth muscle of the CC itself.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alm P., Alumets J., Brodin E., Håkanson R., Nilsson G., Sjöberg N. O., Sundler F. Peptidergic (substance P) nerves in the genito-urinary tract. Neuroscience. 1978;3(4-5):419–425. doi: 10.1016/0306-4522(78)90044-1. [DOI] [PubMed] [Google Scholar]
  2. BALIS J. U., HAUST M. D., MORE R. H. ELECTRON-MICROSCOPIC STUDIES IN HUMAN ATHEROSCLEROSIS; CELLULAR ELEMENTS IN AORTIC FATTY STREAKS. Exp Mol Pathol. 1964 Oct;90:511–525. doi: 10.1016/0014-4800(64)90031-0. [DOI] [PubMed] [Google Scholar]
  3. Baumgarten H. G., Holstein A. F., Owman C. Auerbach's plexus of mammals and man: electron microscopic identification of three different types of neuronal processes in myenteric ganglia of the large intestine from rhesus monkeys, guinea-pigs and man. Z Zellforsch Mikrosk Anat. 1970;106(3):376–397. doi: 10.1007/BF00335780. [DOI] [PubMed] [Google Scholar]
  4. Burden H. W., Lawrence I. E., Jr Experimental studies on the acetylcholinesterase-positive nerves in the ovary of the rat. Anat Rec. 1978 Feb;190(2):233–241. doi: 10.1002/ar.1091900207. [DOI] [PubMed] [Google Scholar]
  5. Burnstock G., Dumsday B., Smythe A. Atropine resistant excitation of the urinary bladder: the possibility of transmission via nerves releasing a purine nucleotide. Br J Pharmacol. 1972 Mar;44(3):451–461. doi: 10.1111/j.1476-5381.1972.tb07283.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Burnstock G. Purinergic nerves. Pharmacol Rev. 1972 Sep;24(3):509–581. [PubMed] [Google Scholar]
  7. CONTI G. L'érection du pénis humain et ses bases morphologico-vasculaires. Acta Anat (Basel) 1952;14(3):217–262. [PubMed] [Google Scholar]
  8. CONTI G. L'érection du pénis humain et ses bases morphologico-vasculaires. Acta Anat (Basel) 1952;14(3):217–262. [PubMed] [Google Scholar]
  9. FLOREY H. W., GREER S. J., POOLE J. C., WERTHESSEN N. T. The pseudointima lining fabric grafts of the aorta. Br J Exp Pathol. 1961 Jun;42:236–246. [PMC free article] [PubMed] [Google Scholar]
  10. HAUST M. D., MORE R. H., MOVAT H. Z. The role of smooth muscle cells in the fibrogenesis of arteriosclerosis. Am J Pathol. 1960 Oct;37:377–389. [PMC free article] [PubMed] [Google Scholar]
  11. Hodgson B. J., Dumas S., Bolling D. R., Heesch C. M. Effect of estrogen on sensitivity of rabbit bladder and urethra to phenylephrine. Invest Urol. 1978 Jul;16(1):67–69. [PubMed] [Google Scholar]
  12. Irey N. S., McAllister H. A., Henry J. M. Oral contraceptives and stroke in young women: a clinicopathologic correlation. Neurology. 1978 Dec;28(12):1216–1219. doi: 10.1212/wnl.28.12.1216. [DOI] [PubMed] [Google Scholar]
  13. KARNOVSKY M. J., ROOTS L. A "DIRECT-COLORING" THIOCHOLINE METHOD FOR CHOLINESTERASES. J Histochem Cytochem. 1964 Mar;12:219–221. doi: 10.1177/12.3.219. [DOI] [PubMed] [Google Scholar]
  14. Klinge E., Sjöstrand N. O. Comparative study of some isolated mammalian smooth muscle effectors of penile erection. Acta Physiol Scand. 1977 Jul;100(3):354–367. doi: 10.1111/j.1748-1716.1977.tb05961.x. [DOI] [PubMed] [Google Scholar]
  15. Leeson T. S., Leeson C. R. The fine structure of cavernous tissue in the adult rat penis. Invest Urol. 1965 Sep;3(2):144–154. [PubMed] [Google Scholar]
  16. McConnell J., Benson G. S., Wood J. Autonomic innervation of the mammalian penis: a histochemical and physiological study. J Neural Transm. 1979;45(3):227–238. doi: 10.1007/BF01244411. [DOI] [PubMed] [Google Scholar]
  17. Mroz E. A., Leeman S. E. Substance P. Vitam Horm. 1977;35:209–281. doi: 10.1016/s0083-6729(08)60524-5. [DOI] [PubMed] [Google Scholar]
  18. Pickel V. M., Reis D. J., Leeman S. E. Ultrastructural localization of substance P in neurons of rat spinal cord. Brain Res. 1977 Feb 25;122(3):534–540. doi: 10.1016/0006-8993(77)90463-2. [DOI] [PubMed] [Google Scholar]
  19. Puchtler H., Sweat F., Terry M. S., Conner H. M. Investigation of staining, polarization and fluorescence-microscopic properties of myoendothelial cells. J Microsc. 1969;89(1):95–104. doi: 10.1111/j.1365-2818.1969.tb00653.x. [DOI] [PubMed] [Google Scholar]
  20. Raz S., Zeigler M., Caine M. The effect of progesterone on the adrenergic receptors of the urethra. Br J Urol. 1973 Apr;45(2):131–135. doi: 10.1111/j.1464-410x.1973.tb12129.x. [DOI] [PubMed] [Google Scholar]
  21. Reale E., Ruska H. Die Feinstrucktur der Gefässwände. Angiologica. 1965;2(4):314–366. [PubMed] [Google Scholar]
  22. Ruzbarský V., Michal V. Morphologic changes in the arterial bed of the penis with aging. Relationship to the pathogenesis of impotence. Invest Urol. 1977 Nov;15(3):194–199. [PubMed] [Google Scholar]
  23. Schreiter F., Fuchs P., Stockamp K. Estrogenic sensitivity of alpha-receptors in the urethra musculature. Urol Int. 1976;31(1-2):13–19. doi: 10.1159/000280026. [DOI] [PubMed] [Google Scholar]
  24. Shira M., Sasaki K., Rikimaru A. Histochemical investigation on the distribution of adrenergic and cholinergic nerves in human penis. Tohoku J Exp Med. 1972 Aug;107(4):403–404. doi: 10.1620/tjem.107.403. [DOI] [PubMed] [Google Scholar]
  25. Torre J. C., Surgeon J. W. A methodological approach to rapid and sensitive monoamine histofluorescence using a modified glyoxylic acid technique: the SPG method. Histochemistry. 1976 Oct 22;49(2):81–93. doi: 10.1007/BF00495672. [DOI] [PubMed] [Google Scholar]
  26. WHITELAW G. P., SMITHWICK R. H. Some secondary effects of sympathectomy; with particular reference to disturbance of sexual function. N Engl J Med. 1951 Jul 26;245(4):121–130. doi: 10.1056/NEJM195107262450401. [DOI] [PubMed] [Google Scholar]
  27. Weiss H. D. The physiology of human penile erection. Ann Intern Med. 1972 May;76(5):793–799. doi: 10.7326/0003-4819-76-5-793. [DOI] [PubMed] [Google Scholar]
  28. Wood J. G. Electron localization of amines in central nervous tissue. Nature. 1966 Mar 12;209(5028):1131–1133. doi: 10.1038/2091131b0. [DOI] [PubMed] [Google Scholar]

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