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. 1980 Jun;65(6):1483–1490. doi: 10.1172/JCI109813

Turnover of Lipoprotein (a) in Man

Franz Krempler 1,2, Gerhard M Kostner 1,2, Klaus Bolzano 1,2, Friedrich Sandhofer 1,2
PMCID: PMC371487  PMID: 7410552

Abstract

An elevated concentration of lipoprotein (a) [Lp(a)] in the serum has been considered a risk factor for coronary heart disease by various investigators. In the present study, the turnover of Lp(a) was investigated in nine individuals with serum Lp(a) levels ranging from 1 to 68 mg/100 ml. After intravenous injection of radioiodinated Lp(a), the radioactivity time-curve of the serum and the specific activitity time-curves of the isolated Lp(a) and Lp(a) apolipoproteins were measured for 14 d. More than 97% of the label was found in the protein moiety of Lp(a). During the entire study period, the serum radioactivity remained with Lp(a), only insignificant amounts of radioactivity were detectable in other lipoprotein fractions. The serum radioactivity time-curves and the specific activity time-curves of the isolated Lp(a) and Lp(a) apolipoproteins were identical.

The kinetic parameters of Lp(a) turnover were calculated in terms of a two-compartment model. 76.5±5.1% (mean±1 SD) of total Lp(a) was contained in the intravascular space. The biological half-life of Lp(a) was 3.32±0.52 d, the fractional catabolic rate (FCR) was 0.306±0.054/d, and the rate of synthesis was 5.00±3.37 mg/kg/d. A positive correlation was found between serum concentration and synthetic rate of Lp(a) apoprotein. No relationship could be demonstrated between serum level and FCR of Lp(a).

The results of this study indicate that Lp(a) is not converted to other serum lipoproteins. From the correlations between serum concentration and kinetic parameters of Lp(a) it is concluded that an elevated Lp(a) level is the consequence of an increased Lp(a) apoprotein synthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albers J. J., Adolphson J. L., Hazzard W. R. Radioimmunoassay of human plasma Lp(a) lipoprotein. J Lipid Res. 1977 May;18(3):331–338. [PubMed] [Google Scholar]
  2. Albers J. J., Cabana V. G., Warnick G. R., Hazzard W. R. Lp(a) lipoprotein: relationship to sinking pre-beta lipoprotein hyperlipoproteinemia, and apolipoprotein B. Metabolism. 1975 Sep;24(9):1047–1054. doi: 10.1016/0026-0495(75)90098-0. [DOI] [PubMed] [Google Scholar]
  3. Albers J. J., Chen C. H., Aladjem F. Human serum lipoproteins. Evidence for three classes of lipoproteins in S fO-2. Biochemistry. 1972 Jan 4;11(1):57–63. doi: 10.1021/bi00751a011. [DOI] [PubMed] [Google Scholar]
  4. Albers J. J., Hazzard W. R. Immunochemical quantification of human plasma Lp(a) lipoprotein. Lipids. 1974 Jan;9(1):15–26. doi: 10.1007/BF02533209. [DOI] [PubMed] [Google Scholar]
  5. BERG K. A NEW SERUM TYPE SYSTEM IN MAN--THE LP SYSTEM. Acta Pathol Microbiol Scand. 1963;59:369–382. doi: 10.1111/j.1699-0463.1963.tb01808.x. [DOI] [PubMed] [Google Scholar]
  6. Berg K., Dahlén G., Frick M. H. Lp(a) lipoprotein and pre-beta1-lipoprotein in patients with coronary heart disease. Clin Genet. 1974;6(3):230–235. doi: 10.1111/j.1399-0004.1974.tb00657.x. [DOI] [PubMed] [Google Scholar]
  7. Bilheimer D. W., Eisenberg S., Levy R. I. The metabolism of very low density lipoprotein proteins. I. Preliminary in vitro and in vivo observations. Biochim Biophys Acta. 1972 Feb 21;260(2):212–221. doi: 10.1016/0005-2760(72)90034-3. [DOI] [PubMed] [Google Scholar]
  8. Dahlén G., Berg K., Ramberg U. B., Tamm A. Lp(a) lipoprotein and pre-beta1-lipoprotein in young adults. Acta Med Scand. 1974 Oct;196(4):327–331. doi: 10.1111/j.0954-6820.1974.tb01018.x. [DOI] [PubMed] [Google Scholar]
  9. Dahlén G., Ericson C., Furberg C., Lundkvist L., Svärdsudd K. Studies on an extra pre-beta lipoprotein fraction. Acta Med Scand Suppl. 1972;531:1–29. [PubMed] [Google Scholar]
  10. EDELMAN I. S., LEIBMAN J. Anatomy of body water and electrolytes. Am J Med. 1959 Aug;27:256–277. doi: 10.1016/0002-9343(59)90346-8. [DOI] [PubMed] [Google Scholar]
  11. Eggstein M., Kreutz F. H. Eine neue Bestimmung der Neutralfette im Blutserum und Gewebe. I. Prinzip, Durchführung und Besprechung der Methode. Klin Wochenschr. 1966 Mar 1;44(5):262–267. doi: 10.1007/BF01747716. [DOI] [PubMed] [Google Scholar]
  12. Ehnholm C., Garoff H., Renkonen O., Simons K. Protein and carbohydrate composition of Lp(a)lipoprotein from human plasma. Biochemistry. 1972 Aug 15;11(17):3229–3232. doi: 10.1021/bi00767a015. [DOI] [PubMed] [Google Scholar]
  13. Ehnholm C., Garoff H., Simons K., Aro H. Purification and quantitation of the human plasma lipoprotein carrying the Lp(a) antigen. Biochim Biophys Acta. 1971 May 25;236(2):431–439. doi: 10.1016/0005-2795(71)90223-6. [DOI] [PubMed] [Google Scholar]
  14. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  15. Kostner G. M., Patsch J. R., Sailer S., Braunsteiner H., Holasek A. Polypeptide distribution of the main lipoprotein density classes separated from human plasma by rate zonal ultracentrifugation. Eur J Biochem. 1974 Jun 15;45(2):611–621. doi: 10.1111/j.1432-1033.1974.tb03587.x. [DOI] [PubMed] [Google Scholar]
  16. Krempler F., Kostner G., Bolzano K., Sandhofer F. Lipoprotein (a) is not a metabolic product of other lipoproteins containing apolipoprotein B. Biochim Biophys Acta. 1979 Oct 26;575(1):63–70. doi: 10.1016/0005-2760(79)90131-0. [DOI] [PubMed] [Google Scholar]
  17. Krempler F., Kostner G., Bolzano K., Sandhofer F. Studies on the metabolism of the lipoprotein Lp (a) in man. Atherosclerosis. 1978 May;30(1):57–65. doi: 10.1016/0021-9150(78)90152-1. [DOI] [PubMed] [Google Scholar]
  18. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  19. Langer T., Strober W., Levy R. I. The metabolism of low density lipoprotein in familial type II hyperlipoproteinemia. J Clin Invest. 1972 Jun;51(6):1528–1536. doi: 10.1172/JCI106949. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Laurell C. B. Electroimmuno assay. Scand J Clin Lab Invest Suppl. 1972;124:21–37. doi: 10.3109/00365517209102748. [DOI] [PubMed] [Google Scholar]
  21. MATTHEWS C. M. The theory of tracer experiments with 131I-labelled plasma proteins. Phys Med Biol. 1957 Jul;2(1):36–53. doi: 10.1088/0031-9155/2/1/305. [DOI] [PubMed] [Google Scholar]
  22. Packard C. J., Third J. L., Shepherd J., Lorimer A. R., Morgan H. G., Lawrie T. D. Low density lipoprotein metabolism in a family of familial hypercholesterolemic patients. Metabolism. 1976 Sep;25(9):995–1006. doi: 10.1016/0026-0495(76)90129-3. [DOI] [PubMed] [Google Scholar]
  23. STEINFELD J. L., PATON R. R., FLICK A. L., MILCH R. A., BEACH F. E., TABERN D. L. Distribution and degradation of human serum albumin labeled with I 131 by different techniques. Ann N Y Acad Sci. 1957 Aug 30;70(1):109–121. doi: 10.1111/j.1749-6632.1957.tb35382.x. [DOI] [PubMed] [Google Scholar]
  24. Schultz J. S., Schreffler D. C., Sing C. F., Harvie N. R. The genetics of the Lp antigen. I. Its quantitation and distribution in a sample population. Ann Hum Genet. 1974 Jul;38(1):39–46. doi: 10.1111/j.1469-1809.1974.tb01991.x. [DOI] [PubMed] [Google Scholar]
  25. Sigurdsson G., Nicoll A., Lewis B. The metabolism of low density lipoprotein in endogenous hypertriglyceridaemia. Eur J Clin Invest. 1976 Mar 31;6(2):151–158. doi: 10.1111/j.1365-2362.1976.tb00506.x. [DOI] [PubMed] [Google Scholar]
  26. Simons K., Ehnholm C., Renkonen O., Bloth B. Characterization of the Lp(a) lipoprotein in human plasma. Acta Pathol Microbiol Scand B Microbiol Immunol. 1970;78(4):459–466. doi: 10.1111/j.1699-0463.1970.tb04328.x. [DOI] [PubMed] [Google Scholar]
  27. Simons L. A., Reichl D., Myant N. B., Mancini M. The metabolism of the apoprotein of plasma low density lipoprotein in familial hyperbetalipoproteinaemia in the homozygous form. Atherosclerosis. 1975 Mar-Apr;21(2):283–298. doi: 10.1016/0021-9150(75)90087-8. [DOI] [PubMed] [Google Scholar]
  28. Utermann G., Lipp K., Wiegandt H. Studies on the Lp(a)-lipoprotein of human serum. IV. The disaggregation of the Lp(a)-lipoprotein. Humangenetik. 1972;14(2):142–150. doi: 10.1007/BF00273299. [DOI] [PubMed] [Google Scholar]

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