Abstract
In previous studies of two patients with polycythemia vera (PV) who were heterozygous at the X-linked locus for glucose-6-phosphate dehydrogenase (G6PD), only A type enzyme was found in nonlymphoid blood cells. However, some erythroid and granulocytic colonies grown in vitro were type B and therefore arose from presumably normal progenitors. One patient had enough type B colonies (8%) that studies of the physical characteristics of normal and PV clonal colony-forming cells could be undertaken. When marrow cells were separated by velocity sedimentation at unit gravity, most PV clonal granulocyte-macrophage progenitors (CFU-C) (type A G6PD) sedimented between 6.4 and 7.2 mm/h, whereas most residual normal, type B CFU-C sedimented less than or equal to 5.9 mm/h (P = 0.04)., When blood cells were separated over a discontinuous buoyant density gradient, PV clonal CFU-C equilibrated at densities < 1.065 g/ml, whereas residual normal CFU-C were found greater than or equal to 1.065 g/ml (P < 0.01). PV clonal and residual normal erythroid burst-forming progenitors were not separable by either method. Thus PV clonal CFU-C are larger and less dense cells than are residual normal CFU-C.
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Selected References
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- Adamson J. W., Fialkow P. J., Murphy S., Prchal J. F., Steinmann L. Polycythemia vera: stem-cell and probable clonal origin of the disease. N Engl J Med. 1976 Oct 21;295(17):913–916. doi: 10.1056/NEJM197610212951702. [DOI] [PubMed] [Google Scholar]
- Fialkow P. J. Primordial cell pool size and lineage relationships of five human cell types. Ann Hum Genet. 1973 Jul;37(1):39–48. doi: 10.1111/j.1469-1809.1973.tb01813.x. [DOI] [PubMed] [Google Scholar]
- Miller R. G., Phillips R. A. Separation of cells by velocity sedimentation. J Cell Physiol. 1969 Jun;73(3):191–201. doi: 10.1002/jcp.1040730305. [DOI] [PubMed] [Google Scholar]
- Moore M. A., Williams N., Metcalf D. In vitro colony formation by normal and leukemic human hematopoietic cells: characterization of the colony-forming cells. J Natl Cancer Inst. 1973 Mar;50(3):603–623. doi: 10.1093/jnci/50.3.603. [DOI] [PubMed] [Google Scholar]
- Prchal J. F., Adamson J. W., Murphy S., Steinmann L., Fialkow P. J. Polycythemia vera. The in vitro response of normal and abnormal stem cell lines to erythropoietin. J Clin Invest. 1978 Apr;61(4):1044–1047. doi: 10.1172/JCI109003. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Singer J. W., Adamson J. W. Steroids and hematopoiesis. II. The effect of steroids on in vitro erythroid colony growth: evidence for different target cells for different classes of steroids. J Cell Physiol. 1976 Jun;88(2):135–143. doi: 10.1002/jcp.1040880203. [DOI] [PubMed] [Google Scholar]
- Singer J. W., Fialkow P. J., Adamson J. W., Steinmann L., Ernst C., Murphy S., Kopecky K. J. Polycythemia vera. Increased expression of normal committed granulocytic stem cells in vitro after exposure of marrow to tritiated thymidine. J Clin Invest. 1979 Nov;64(5):1320–1324. doi: 10.1172/JCI109588. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Singer J. W., Fialkow P. J., Dow L. W., Ernst C., Steinmann L. Unicellular or multicellular origin of human granulocyte-macrophage colonies in vitro. Blood. 1979 Dec;54(6):1395–1399. [PubMed] [Google Scholar]