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. 1978 Oct;62(4):879–883. doi: 10.1172/JCI109200

Failure to detect an effect of prolactin on pulmonary surfactant and adrenal steroids in fetal sheep and rabbits.

P L Ballard, P D Gluckman, A Brehier, J A Kitterman, S L Kaplan, A M Rudolph, M M Grumbach
PMCID: PMC371840  PMID: 581289

Abstract

Recent reports have indicated an association between low cord prolactin (PRL) and the occurrence of respiratory distress syndrome in premature infants, and it is reported that PRL administration increases the lecithin content of fetal rabbit lung. We administered 1 mg ovine PRL to 32 rabbit fetuses on day 24 of gestation and evaluated lung phospholipid synthesis and content on day 26. Compared with diluent-injected littermates, PRL had no effect on the rate of choline incorporation into lecithin, tissue content of phospholipid and disaturated lecithin, or plasma corticoids. However, both choline incorporation and corticoids were increased in all animals undergoing surgery compared with unoperated controls. We also infused PRL (1 mg/day, i.v.) into three fetal sheep continuously over five periods of 5-8 days. Although supraphysiologic concentrations of PRL were achieved in plasma and amniotic fluid, there was no effect of this treatment on the flux of tracheal fluid surfactant or on plasma concentrations of corticoids of dehydroepiandrosterone sulfate. Thus, in this study, we failed to detect either a stimulation of the surfactant system or an adreno-corticotropic effect by PRL as previously postulated. This suggests that the relationship between PRL and respiratory distress sundrome is an indirect association.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aubert M. J., Grumbach M. M., Kaplan S. L. The ontogenesis of human fetal hormones. III. Prolactin. J Clin Invest. 1975 Jul;56(1):155–164. doi: 10.1172/JCI108064. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Avery M. E. Pharmacological approaches to the acceleration of fetal lung matturation. Br Med Bull. 1975 Jan;31(1):13–17. [PubMed] [Google Scholar]
  3. BARTLETT G. R. Phosphorus assay in column chromatography. J Biol Chem. 1959 Mar;234(3):466–468. [PubMed] [Google Scholar]
  4. Ballard R. A., Ballard P. L. Use of prenatal glucocorticoid therapy to prevent respiratory distress syndrome. A supporting view. Am J Dis Child. 1976 Sep;130(9):982–987. doi: 10.1001/archpedi.1976.02120100072011. [DOI] [PubMed] [Google Scholar]
  5. Brehier A., Benson B. J., Williams M. C., Mason R. J., Ballard P. L. Corticosteroid induction of phosphatidic acid phosphatase in fetal rabbit lung. Biochem Biophys Res Commun. 1977 Aug 8;77(3):883–890. doi: 10.1016/s0006-291x(77)80060-0. [DOI] [PubMed] [Google Scholar]
  6. Conly P. W., LeMaire W. J., Monkus E. F., Cleveland W. W. Plasma estriol concentration in infants with the respiratory distress syndrome. J Pediatr. 1973 Nov;83(5):851–853. doi: 10.1016/s0022-3476(73)80388-9. [DOI] [PubMed] [Google Scholar]
  7. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  8. Hamosh M., Hamosh P. The effect of prolactin on the lecithin content of fetal rabbit lung. J Clin Invest. 1977 May;59(5):1002–1005. doi: 10.1172/JCI108693. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hauth J. C., Parker C. R., Jr, MacDonald P. C., Porter J. C., Johnston J. M. A role of fetal prolactin in lung maturation. Obstet Gynecol. 1978 Jan;51(1):81–88. [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Lowe K. C., Jansen C. A., Thomas A. L., Nathanielsz P. W. Effect of prolactin infusion into the chronically catheterized ovine foetus [proceedings]. J Endocrinol. 1977 Dec;75(3):55P–56P. [PubMed] [Google Scholar]
  12. Mason R. J., Nellenbogen J., Clements J. A. Isolation of disaturated phosphatidylcholine with osmium tetroxide. J Lipid Res. 1976 May;17(3):281–284. [PubMed] [Google Scholar]
  13. Mescher E. J., Platzker A. C., Ballard P. L., Kitterman J. A., Clements J. A., Tooley W. H. Ontogeny of tracheal fluid, pulmonary surfactant, and plasma corticoids in the fetal lamb. J Appl Physiol. 1975 Dec;39(6):1017–1021. doi: 10.1152/jappl.1975.39.6.1017. [DOI] [PubMed] [Google Scholar]
  14. Murphy B. E. Some studies of the protein-binding of steroids and their application to the routine micro and ultramicro measurement of various steroids in body fluids by competitive protein-binding radioassay. J Clin Endocrinol Metab. 1967 Jul;27(7):973–990. doi: 10.1210/jcem-27-7-973. [DOI] [PubMed] [Google Scholar]
  15. Platzker A. C., Kitterman J. A., Mescher E. J., Clements J. A., Tooley W. H. Surfactant in the lung and tracheal fluid of the fetal lamb and acceleration of its appearance by dexamethasone. Pediatrics. 1975 Oct;56(4):554–561. [PubMed] [Google Scholar]
  16. Shiu R. P., Kelly P. A., Friesen H. G. Radioreceptor assay for prolactin and other lactogenic hormones. Science. 1973 Jun 1;180(4089):968–971. doi: 10.1126/science.180.4089.968. [DOI] [PubMed] [Google Scholar]
  17. Winters A. J., Colston C., MacDonald P. C., Porter J. C. Fetal plasma prolactin levels. J Clin Endocrinol Metab. 1975 Sep;41(3):626–629. doi: 10.1210/jcem-41-3-626. [DOI] [PubMed] [Google Scholar]
  18. Wu B., Kikkawa Y., Orzalesi M. M., Motoyama E. K., Kaibara M., Zigas C. J., Cook C. D. The effect of thyroxine on the maturation of fetal rabbit lungs. Biol Neonate. 1973;22(3):161–168. doi: 10.1159/000240550. [DOI] [PubMed] [Google Scholar]

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